CAJ | 학술논문

目的：比较手性修饰的氧化石墨（GO ）衍生物对小鼠成神经瘤细胞 N2a活性的影响。方法倒置荧光显微镜观察N2a细胞形态，M T T法检测N2a细胞的活性。结果在0．1～0．4μmol／L浓度GO衍生物对N2a细胞活性影响呈浓度相关，其中0．4μmol／L的GO衍生物对N2a细胞生长状况最好；L‐型GO （L‐GO ）和D‐型GO （D‐GO）促N2a细胞增殖率分别为94％和52％，二者差异有统计学意义（P＜0．01）。结论手性修饰的GO衍生物在0．1～0．4μmol／L能促进N2a细胞生长，L‐GO促进N2a细胞生长活性比D‐GO强。
목적：비교수성수식적양화석묵（GO ）연생물대소서성신경류세포 N2a활성적영향。방법도치형광현미경관찰N2a세포형태，M T T법검측N2a세포적활성。결과재0．1～0．4μmol／L농도GO연생물대N2a세포활성영향정농도상관，기중0．4μmol／L적GO연생물대N2a세포생장상황최호；L‐형GO （L‐GO ）화D‐형GO （D‐GO）촉N2a세포증식솔분별위94％화52％，이자차이유통계학의의（P＜0．01）。결론수성수식적GO연생물재0．1～0．4μmol／L능촉진N2a세포생장，L‐GO촉진N2a세포생장활성비D‐GO강。
Objective To compare the influence of chirally modified graphite oxide (GO) derivatives on the ac‐tivity of mouse neuroblastoma N2a cells .Methods The morphological change of N2a cells was observed by the inver‐sion fluorescence microscope and the activity of N2a cells was measured by the MTT method .Results The influence of GO derivatives in the concentration range of 0 .1-0 .4 μmol/L on the activity of N2a cells showed the concentra‐tion correlation ,in which the GO derivatives with the concentration of 0 .4μmol/L was best for the growth status of the N2a cells ;the promoting N2a cells proliferation rates of the L‐type GO and D‐type GO were 94% and 52% re‐spectively ,the difference between them revealed the statistical significance(P<0 .01) .Conclusion The chirally modi‐fied GO derivatives in the concentration range of 0 .1-0 .4μmol/L could promote N2a cell growth ,and the activity of L‐GO promoting N2a cell growth is stronger than that of D‐GO .