CAJ | 학술논문

目的：研究基质金属蛋白酶‐9（MMP‐9）与血管内皮细胞生长因子（VEGF）在肺腺癌表达的相关性及意义。方法应用免疫组织化学（SP）法检测MMP‐9、VEGF在30例肺腺癌组织和12例癌旁正常组织中的表达。Western blot检测MMP‐9单克隆抗体阻断MMP‐9后肺腺癌A549细胞VEGF蛋白表达水平的变化，以及VEGF克隆抗体阻断VEGF后肺腺癌 A549细胞 MMP‐9蛋白表达水平的变化。结果 MMP‐9在腺癌组织表达率为63．3％，正常组织表达率为16．7％，差异有统计学意义（P＜0．05）；VEGF在腺癌组织表达率为70％，正常组织表达率为25％，差异有统计学意义（P＜0．05）；肺腺癌组织MMP‐9水平与VEGF表达率存在正相关关系（r＝0．7094， P＜0．01），并与肺腺癌的临床分期及淋巴转移状况相关（P＜0．05）。VEGF高表达率与肺腺癌的临床分期及淋巴转移状况相关（P＜0．05）。用MMP‐9单克隆抗体阻断MMP‐9可降低VEGF蛋白表达水平，用VEGF单克隆抗体阻断VEGF可降低MMP‐9蛋白表达水平。结论 VEGF、MMP‐9在肺腺癌高表达，在促进腺癌侵袭转移中具有协同作用。
목적：연구기질금속단백매‐9（MMP‐9）여혈관내피세포생장인자（VEGF）재폐선암표체적상관성급의의。방법응용면역조직화학（SP）법검측MMP‐9、VEGF재30례폐선암조직화12례암방정상조직중적표체。Western blot검측MMP‐9단극륭항체조단MMP‐9후폐선암A549세포VEGF단백표체수평적변화，이급VEGF극륭항체조단VEGF후폐선암 A549세포 MMP‐9단백표체수평적변화。결과 MMP‐9재선암조직표체솔위63．3％，정상조직표체솔위16．7％，차이유통계학의의（P＜0．05）；VEGF재선암조직표체솔위70％，정상조직표체솔위25％，차이유통계학의의（P＜0．05）；폐선암조직MMP‐9수평여VEGF표체솔존재정상관관계（r＝0．7094， P＜0．01），병여폐선암적림상분기급림파전이상황상관（P＜0．05）。VEGF고표체솔여폐선암적림상분기급림파전이상황상관（P＜0．05）。용MMP‐9단극륭항체조단MMP‐9가강저VEGF단백표체수평，용VEGF단극륭항체조단VEGF가강저MMP‐9단백표체수평。결론 VEGF、MMP‐9재폐선암고표체，재촉진선암침습전이중구유협동작용。
Objective To investigate the correlation between matrix metalloprotoinase‐9(MMP‐9) and vascu‐lar endothelial growth factor(VEGF) expressions in lung adenocarcinoma and its significance .Methods The immu‐nohistochemistry (SP) was used to test the expressions of MMP‐9 and VEGF in 30 specimens of lung adenocarcino‐ma and 12 specimens of normal paracancerous lung tissues .Western blot was used to detect the change of VEGF pro‐tein levels in lung adenocarcinoma A549 cells after blocking MMP‐9 by MMP‐9 mAb ,and the change of MMP‐9 pro‐tein levels in lung adenocarcinoma A549 cells after blocking VEGF by VEGF mAb .Results The high expression rate of MMP‐9 protein in lung adenocarcinoma tissues was 63 .3% ,which in the normal tissues was 16 .7% ,the difference had statistical significance(P<0 .05);the high expression rate of VEGF protein in lung adenocarcinoma tissues was 70% ,which in the normal tissues was 25% ,the difference had statistical significance(P<0 .05);the high expression rate of MMP‐9 in the lung adenocarcinoma was positively correlated with the high expression rate of VEGF(r=0 .709 4 ,P<0 .01) ,and which was correlated with the lymph node metastasis status and the clinical TNM stage(P<0 .05) .The high expression rate of VEGF was correlated with the lymph node metastasis status and the clinical TNM stage(P<0 .05) .Blocking MMP‐9 by MMP‐9 mAb could reduce the VEGF protein expression level .Blocking VEGF by VEGF mAb could reduce the MMP‐9 protein expression level .Conclusion VEGF and MMP‐9 are highly ex‐pressed in lung adenocarcinoma and have the synergistic action in promoting invasion and metastasis of lung adeno‐carcinoma .