CAJ | 학술논문

目的：研究抑癌基因CYLD (cylindromatosis)、原癌基因c-jun及细胞核增殖相关抗原Ki-67在中耳胆脂瘤上皮细胞中的表达情况，分析它们之间的相互关系，探讨CYLD、c-jun、Ki-67在中耳胆脂瘤发病机制中的可能作用。方法采用免疫组化技术检测CYLD、c-jun及Ki-67蛋白产物在30例中耳胆脂瘤上皮组织（实验组）及20例正常耳后皮肤上皮组织（对照组）中的表达情况。结果 CYLD蛋白产物阳性表达定位于细胞浆，其在中耳胆脂瘤上皮中主要见基底层弱阳性或阳性表达，而在对照组正常耳后皮肤上皮全层均见阳性表达或强阳性表达, CYLD蛋白在中耳胆脂瘤上皮中的表达明显低于正常耳后皮肤（X2=16.333, p=0.000, p<0.05）。c-jun蛋白产物阳性表达定位于细胞浆和细胞核，其在中耳胆脂瘤上皮中上皮全层均见阳性表达或强阳性表达，而在对照组正常耳后皮肤上皮中均仅见基底层弱阳性或阳性表达，c-jun蛋白在中耳胆脂瘤上皮中的表达明显高于正常耳后皮肤（X2=14.901, p=0.000, p<0.05）。Ki-67蛋白产物定位于细胞核，细胞质内未见Ki-67蛋白表达。其在中耳胆脂瘤上皮基底层、棘层，甚至颗粒层中均可见阳性或强阳性表达，而在对照组正常耳后皮肤上皮中均仅见基底层弱阳性或阳性表达，Ki-67蛋白在中耳胆脂瘤上皮中的表达明显高于正常耳后皮肤（X2=13.675,P=0.000,P<0.05）。Spearman相关性分析发现中耳胆脂瘤上皮细胞CYLD、c-jun蛋白表达存在负相关（rs=-0.381, P=0.038,P<0.05）。结论与正常耳后皮肤的上皮细胞相比，中耳胆脂瘤上皮细胞具有高度增殖的能力。CYLD、c-jun异常表达可能与中耳胆脂瘤发生、发展密切相关。在中耳胆脂瘤上皮中可能也存在CYLD对c-jun表达的负调节。
목적：연구억암기인CYLD (cylindromatosis)、원암기인c-jun급세포핵증식상관항원Ki-67재중이담지류상피세포중적표체정황，분석타문지간적상호관계，탐토CYLD、c-jun、Ki-67재중이담지류발병궤제중적가능작용。방법채용면역조화기술검측CYLD、c-jun급Ki-67단백산물재30례중이담지류상피조직（실험조）급20례정상이후피부상피조직（대조조）중적표체정황。결과 CYLD단백산물양성표체정위우세포장，기재중이담지류상피중주요견기저층약양성혹양성표체，이재대조조정상이후피부상피전층균견양성표체혹강양성표체, CYLD단백재중이담지류상피중적표체명현저우정상이후피부（X2=16.333, p=0.000, p<0.05）。c-jun단백산물양성표체정위우세포장화세포핵，기재중이담지류상피중상피전층균견양성표체혹강양성표체，이재대조조정상이후피부상피중균부견기저층약양성혹양성표체，c-jun단백재중이담지류상피중적표체명현고우정상이후피부（X2=14.901, p=0.000, p<0.05）。Ki-67단백산물정위우세포핵，세포질내미견Ki-67단백표체。기재중이담지류상피기저층、극층，심지과립층중균가견양성혹강양성표체，이재대조조정상이후피부상피중균부견기저층약양성혹양성표체，Ki-67단백재중이담지류상피중적표체명현고우정상이후피부（X2=13.675,P=0.000,P<0.05）。Spearman상관성분석발현중이담지류상피세포CYLD、c-jun단백표체존재부상관（rs=-0.381, P=0.038,P<0.05）。결론여정상이후피부적상피세포상비，중이담지류상피세포구유고도증식적능력。CYLD、c-jun이상표체가능여중이담지류발생、발전밀절상관。재중이담지류상피중가능야존재CYLD대c-jun표체적부조절。
Objective To study expression of the tumor suppressor gene CYLD (cylindromatosis), proto-oncogene c-jun and proliferating cell nuclear antigen Ki-67 in middle ear cholesteatoma epithelium, and their correlation, as well as their possible roles in the pathogenesis of middle ear cholesteatoma. Method Immunohistochemical techniques were used to detect expression of CYLD, c-jun and Ki-67 in middle ear cholesteatoma tissues from 30 patients (the experimental group) and in normal retroauricular skin from 20 of the patients (the control group). Results CYLD immunoreactivity was detected in the cytoplasm of epithelial cells. The reaction was mildly positive and mainly in the basal layer in middle ear cholesteatoma, while strong in all epithelial layers in the control group (normal retroauricular skin) (X2=14.901, p<0.05). The c-jun immuno?reactivity was detected in the cytoplasm and nuclei of epithelial cells, and positive or strong positive in all epithelial layers in middle ear cholesteatoma, but showing relative weak expression only in the basal layers in the control group (X2=16.333, p<0.05). Ki-67 immunoreactivity was detected in the nuclei of epithelial cells but not in cytoplasm. It showed strong positive ex? pression mainly in the basal and spinous layers (and sporadically in the granularlayer layer) in cholesteatoma epithelium, but relatively weak expression limited to the basal layer in the control groups (X2=13.675, p<0.05). In middle ear cholesteatoma epithelium, CYLD expression showed negative correlation to the expression of c-jun (rs=-0.401, p<0.05). Conclusion Middle ear cholesteatoma epithelium has a high proliferative capacity compared to epithelial cells of the normal skin. The abnormal ex?pression of CYLD and c-jun in middle ear cholesteatoma epithelium suggests their important roles in the formation and devel?opment of cholesteatoma. The negative correlation between CYLD and c-jun expression may exist in middle ear cholesteatoma.