CAJ | 학술논문

目的：利用计算机断层扫描显微成像（Micro-Computed Tomography，Micro-CT）技术拍摄豚鼠听骨链，获得完整清晰的3D图像，精确测量听骨链各组成部分尺寸，探讨豚鼠听骨链解剖结构。方法断头处死豚鼠，沿颅中线剪开头部，取出听泡剥除后内侧壁，苦味酸甲醛溶液固定后，利用Micro-CT技术获取三维图像。应用Avizo建模重构获得三维模型，分割染色听骨链并测量各听小骨组成结构。结果得到空间视觉良好、可任意轴向旋转的豚鼠听骨链3D模型，局部结构清晰，可任意分割显示并测量内外部结构，砧锤复合体、砧锤复合体长脚、砧锤复合体短脚、镫骨底板、镫骨长度、锤骨柄的长度分别为：3960±222μm、1180±36μm、909±11μm；2111±58μm、2496±104μm、3417±58μm；砧锤复合体、镫骨底板的宽度和锤骨柄高度为：1089±71μm、902±13μm、1760±74μm；砧骨镫骨夹角、砧骨锤骨夹角分别为：108.58°±1.26°、122.46°±4.04°。结论采用Micro-CT采集了豚鼠听泡的三维图像，并通过Avizo软件重建，精确分割出豚鼠听骨链的解剖结构并进行了精确测量，为豚鼠听骨链可视化研究提供了清晰直观的观察方式，测量并统计分析了豚鼠听骨链各小骨生理数据，为以豚鼠为对象的进一步听力学研究提供了新的研究方法。
목적：이용계산궤단층소묘현미성상（Micro-Computed Tomography，Micro-CT）기술박섭돈서은골련，획득완정청석적3D도상，정학측량은골련각조성부분척촌，탐토돈서은골련해부결구。방법단두처사돈서，연로중선전개두부，취출은포박제후내측벽，고미산갑철용액고정후，이용Micro-CT기술획취삼유도상。응용Avizo건모중구획득삼유모형，분할염색은골련병측량각은소골조성결구。결과득도공간시각량호、가임의축향선전적돈서은골련3D모형，국부결구청석，가임의분할현시병측량내외부결구，침추복합체、침추복합체장각、침추복합체단각、등골저판、등골장도、추골병적장도분별위：3960±222μm、1180±36μm、909±11μm；2111±58μm、2496±104μm、3417±58μm；침추복합체、등골저판적관도화추골병고도위：1089±71μm、902±13μm、1760±74μm；침골등골협각、침골추골협각분별위：108.58°±1.26°、122.46°±4.04°。결론채용Micro-CT채집료돈서은포적삼유도상，병통과Avizo연건중건，정학분할출돈서은골련적해부결구병진행료정학측량，위돈서은골련가시화연구제공료청석직관적관찰방식，측량병통계분석료돈서은골련각소골생리수거，위이돈서위대상적진일보은역학연구제공료신적연구방법。
Objective The aim of this study was to observe the structure of the ossicular chain of a guinea pig, to obtain a clear 3D image and detect component sizes of the ossicular chain using Micro-CT technology. Methods Behead a guinea pig, cut the head along the median line. Took out the auditory capsule, and removed its postero and inner wall. After fixed it using picroformal solution, we shot it with Micro-CT to gain image. Employed Avizo software to obtain a three-dimensional model, separate the ossicular chain structure, and detect component sizes of the ossicular chain. Results Three-dimensional model was clearly visible and able to rotate around any axis. The structure was partially clear. Inner and outside structure can be observed separately at will. Length of incus, long limb of incus, short limb of incus, stapes footplate, stapes,manubrium mallei were separately 3960±222μm, 1180±36μm, 909±11μm;2111±58μm, 2496±104μm, 3417±58μm, width of incus, sta?pes footplate and height of stapes, manubrium mallei were separately 1089±71μm, 902±13μm, 1760±74μm. Angle between incus and stapes, angle between incus and manubrium mallei:108.58° ± 1.26° , 122.46° ± 4.04° . Conclusion The thesis ac?quired a three-dimensional image of auditory capsule of a guinea pig using Micro-CT, and by employing Avizo software, we precisely separated out the ossicular structure and accurately detected component sizes of the ossicular chain. That offered a direct observation method for the visualized research on the ossicular chain of a guinea pig and a new way for audiology study on guinea pigs.