CAJ | 학술논문

目的：观察人参总皂苷预处理对电离辐射下人造血干细胞（ HPCs ）的保护作用，并探讨其作用机制。方法将HPCs随机分为对照组和观察组，对照组不处理，观察组予以人参皂苷5．0μmol／L培养24 h，分别用剂量为0、1、2、5 Gy的X线对两组细胞进行照射。 MTT法测算细胞存活率，流式细胞仪测定辐射后24 h细胞内的活性氧（ ROS），Western blot 法测定细胞内的Caspase-3和Nrf-2。结果随着辐射剂量的增加，两组细胞存活率均逐渐下降（P均＜0．05），细胞内ROS活性增强、Caspase-3表达增加、Nrf-2表达减少（P均＜0．05）；与相同照射剂量对照组比较，观察组细胞存活率增加（P均＜0．05），细胞内ROS活性减弱、Caspase-3表达减少、Nrf-2表达增加（P均＜0．05）。结论人参皂苷对电离辐射下的HPCs具有保护作用，该作用与其抗凋亡和抑制氧化应激有关。
목적：관찰인삼총조감예처리대전리복사하인조혈간세포（ HPCs ）적보호작용，병탐토기작용궤제。방법장HPCs수궤분위대조조화관찰조，대조조불처리，관찰조여이인삼조감5．0μmol／L배양24 h，분별용제량위0、1、2、5 Gy적X선대량조세포진행조사。 MTT법측산세포존활솔，류식세포의측정복사후24 h세포내적활성양（ ROS），Western blot 법측정세포내적Caspase-3화Nrf-2。결과수착복사제량적증가，량조세포존활솔균축점하강（P균＜0．05），세포내ROS활성증강、Caspase-3표체증가、Nrf-2표체감소（P균＜0．05）；여상동조사제량대조조비교，관찰조세포존활솔증가（P균＜0．05），세포내ROS활성감약、Caspase-3표체감소、Nrf-2표체증가（P균＜0．05）。결론인삼조감대전리복사하적HPCs구유보호작용，해작용여기항조망화억제양화응격유관。
Objective To investigate the protective effect of ginsenoside on hematopoietic progenitor cells ( HPCs ) under different intensity of ionizing radiations .Methods The HPCs were divided into the control group and the observe group, then to add ginsenoside into the observe group and cultivated for 24 hours.Respectively exposed under X-ray irradi-ations on 1, 2, 5 Gy.Cell viability was studied by MTT method .The generation of reactive oxygen species ( ROS) after 24 hours, the expression of caspase-3 mRNA and Nrf-2 mRNA were measured by means of flow cytometry and Western blot , respectively .Results With the increase of radiation dose , cell viability reduced , the generation of ROS increased , the ex-pression of caspase-3 mRNA enhanced and the Nrf-2 mRNA decreased, gradually, all P<0.05.Compared with control group with the same radiation dose , cell viability in the observe group increased , the generation of ROS and the expression of caspase-3 mRNA decreased, the Nrf-2 mRNA increased, all P<0.05.Conclusion Ginsenoside could protect HPCs from radiation effects , which was correlated with anti-apoptosis and inhibition of oxidation stress .