南通大学学报（医学版）
南通大學學報（醫學版）
남통대학학보（의학판）
JOURNAL OF NANTONG UNIVERSITY(MEDICAL SCIENCES)
2015年
1期
13-16
,共4页
高赛楠%张玉泉%苏敏%黄华
高賽楠%張玉泉%囌敏%黃華
고새남%장옥천%소민%황화
卵巢癌%β绒毛膜促性腺激素的第5号亚基%体内研究
卵巢癌%β絨毛膜促性腺激素的第5號亞基%體內研究
란소암%β융모막촉성선격소적제5호아기%체내연구
ovarian cancer%chorionic gonadotropin beta polpeptide 5%in vivo
目的：通过体内实验研究β绒毛膜促性腺激素的第5号亚基(chorionic gonadotropin beta polpeptide 5， CGB5)对卵巢癌细胞OVCAR-3成瘤率及成瘤速度的影响。方法：采用慢病毒载体转染的方法，在卵巢癌细胞OVCAR-3中分别转入CGB5小干扰、过表达及无关序列。另外，未处理的OVCAR-3细胞作为空白对照，将高表达CGB5的绒癌细胞BeWo作为阳性对照。用RT-PCR和酶联免疫吸附试验法测定转染后细胞的CGB5浓度。裸鼠随机分为5组(正常OVCAR-3细胞组，CGB5小干扰组，CGB5过表达组，CGB5无关序列组，BeWo细胞组)，每组6只，将这5种不同的细胞株分别打入裸鼠右侧腋窝皮下建立皮下移植瘤模型。观察5组模型的肿瘤成瘤率及成瘤速度，并用免疫组化测定肿瘤细胞增殖指标Ki67的表达情况。结果：(1)CGB5过表达组和 BeWo 细胞组裸鼠的皮下移植瘤成瘤率是100%(6/6)， OVCAR-3正常细胞组和无关序列对照组细胞的裸鼠的皮下移植瘤成瘤率是83.3%(5/6)，而CGB5小干扰组细胞的裸鼠皮下移植瘤成瘤率是33.3%(2/6)。(2)CGB5过表达组和CGB5表达高的BeWo细胞组的肿瘤生长较快，而CGB5小干扰组的肿瘤生长最慢。结论：CGB5能增加卵巢癌OVCAR-3细胞皮下移植瘤的成瘤率及肿瘤的生长速度，这将有望为卵巢癌的治疗提供新的思路。
目的：通過體內實驗研究β絨毛膜促性腺激素的第5號亞基(chorionic gonadotropin beta polpeptide 5， CGB5)對卵巢癌細胞OVCAR-3成瘤率及成瘤速度的影響。方法：採用慢病毒載體轉染的方法，在卵巢癌細胞OVCAR-3中分彆轉入CGB5小榦擾、過錶達及無關序列。另外，未處理的OVCAR-3細胞作為空白對照，將高錶達CGB5的絨癌細胞BeWo作為暘性對照。用RT-PCR和酶聯免疫吸附試驗法測定轉染後細胞的CGB5濃度。裸鼠隨機分為5組(正常OVCAR-3細胞組，CGB5小榦擾組，CGB5過錶達組，CGB5無關序列組，BeWo細胞組)，每組6隻，將這5種不同的細胞株分彆打入裸鼠右側腋窩皮下建立皮下移植瘤模型。觀察5組模型的腫瘤成瘤率及成瘤速度，併用免疫組化測定腫瘤細胞增殖指標Ki67的錶達情況。結果：(1)CGB5過錶達組和 BeWo 細胞組裸鼠的皮下移植瘤成瘤率是100%(6/6)， OVCAR-3正常細胞組和無關序列對照組細胞的裸鼠的皮下移植瘤成瘤率是83.3%(5/6)，而CGB5小榦擾組細胞的裸鼠皮下移植瘤成瘤率是33.3%(2/6)。(2)CGB5過錶達組和CGB5錶達高的BeWo細胞組的腫瘤生長較快，而CGB5小榦擾組的腫瘤生長最慢。結論：CGB5能增加卵巢癌OVCAR-3細胞皮下移植瘤的成瘤率及腫瘤的生長速度，這將有望為卵巢癌的治療提供新的思路。
목적：통과체내실험연구β융모막촉성선격소적제5호아기(chorionic gonadotropin beta polpeptide 5， CGB5)대란소암세포OVCAR-3성류솔급성류속도적영향。방법：채용만병독재체전염적방법，재란소암세포OVCAR-3중분별전입CGB5소간우、과표체급무관서렬。령외，미처리적OVCAR-3세포작위공백대조，장고표체CGB5적융암세포BeWo작위양성대조。용RT-PCR화매련면역흡부시험법측정전염후세포적CGB5농도。라서수궤분위5조(정상OVCAR-3세포조，CGB5소간우조，CGB5과표체조，CGB5무관서렬조，BeWo세포조)，매조6지，장저5충불동적세포주분별타입라서우측액와피하건립피하이식류모형。관찰5조모형적종류성류솔급성류속도，병용면역조화측정종류세포증식지표Ki67적표체정황。결과：(1)CGB5과표체조화 BeWo 세포조라서적피하이식류성류솔시100%(6/6)， OVCAR-3정상세포조화무관서렬대조조세포적라서적피하이식류성류솔시83.3%(5/6)，이CGB5소간우조세포적라서피하이식류성류솔시33.3%(2/6)。(2)CGB5과표체조화CGB5표체고적BeWo세포조적종류생장교쾌，이CGB5소간우조적종류생장최만。결론：CGB5능증가란소암OVCAR-3세포피하이식류적성류솔급종류적생장속도，저장유망위란소암적치료제공신적사로。
Objective: To explore the effect of chorionic gonadotropin beta polpeptide 5(CGB5) on human epithelial ovarian cancer cell line OVCAR-3 in the tumor formation rates and growth speed in vivo. Methods: The CGB5 overexpressed vector, CGB5 targeted siRNA vector were constructed and transfected into OVCAR-3 ( empty vector as control ) . The human choriocarcinoma BeWo cell line with CGB5 high expressed was used as a positive control. After sorting by flow cytometry, and selected by Blasticidin, the stable vector expression cell lines were obtained. These cells (five groups: untreated OVCAR-3 cells, CGB5 targeted siRNA transfected OVCAR-3 cells, target-off siRNA transfected OVCAR-3 cells, CGB5 overexpressed OVCAR-3 cells and BeWo cells) were injected subcutaneously into the nude mice (6 mice/group). Then the tumor formation rates and growth speed were examined. The expression of Ki67 was detected by the immunohistochemistry. Results: The tumor formation rates of nude mouse xenografts were 100%(6/6) for CGB5 overexpressed OVCAR-3 and BeWo cells;83.3%(5/6) for untreated or target-off siRNA transfected OVCAR-3 cells group;and 33.3%(2/6) for CGB5 knockdown OVCAR-3 cells. And the speeds of tumor growth were also different, BeWo and CGB5 overexpressed OVCAR-3 cells were the growth fastest, but the CGB5 knockdown OVCAR-3 cells were the slowest. Conclusions: CGB5 may increase the tumor formation rates and growth speed, which may support a new way for ovarian cancer therapy.
