CAJ | 학술논문

目的：研究熊果酸（ursolic acid ，UA）对顺铂（cisplatin ，CDDP）致毒小鼠耳蜗瞬时感受器电位香草酸受体1（transient receptor potential vanilloid 1，TRPV1）表达的影响。方法60只健康BALB／c小鼠随机分成对照组（腹腔注射等量生理盐水）、UA组（腹腔注射熊果酸80 mg／kg）、CDDP组（腹腔注射CDDP 4．5 mg／kg）和CDDP＋UA组（一侧腹腔注CDDP 4．5 mg／kg ，同时对侧腹腔注射熊果酸80 mg／kg），每组15只。各组动物均连续腹腔注射给药5天，每天1次，给药前及停药后24 h行ABR检测，然后应用免疫组织化学染色、显微图像分析及蛋白质印迹技术观察小鼠耳蜗中TRPV1的表达。结果 CDDP组小鼠耳蜗TRPV1表达和ABR阈移均较对照组明显增加（P＜0．05），UA＋CDDP组小鼠ABR阈移及TRPV1表达较CDDP组明显降低（P＜0．05），且TRPV1表达变化与ABR阈移改变高度相关（｜r｜＞0．7，P＜0．05）。结论 UA可抑制CDDP所致TRPV1的高表达，有效降低CD_DP的耳毒性，改善听功能。
목적：연구웅과산（ursolic acid ，UA）대순박（cisplatin ，CDDP）치독소서이와순시감수기전위향초산수체1（transient receptor potential vanilloid 1，TRPV1）표체적영향。방법60지건강BALB／c소서수궤분성대조조（복강주사등량생리염수）、UA조（복강주사웅과산80 mg／kg）、CDDP조（복강주사CDDP 4．5 mg／kg）화CDDP＋UA조（일측복강주CDDP 4．5 mg／kg ，동시대측복강주사웅과산80 mg／kg），매조15지。각조동물균련속복강주사급약5천，매천1차，급약전급정약후24 h행ABR검측，연후응용면역조직화학염색、현미도상분석급단백질인적기술관찰소서이와중TRPV1적표체。결과 CDDP조소서이와TRPV1표체화ABR역이균교대조조명현증가（P＜0．05），UA＋CDDP조소서ABR역이급TRPV1표체교CDDP조명현강저（P＜0．05），차TRPV1표체변화여ABR역이개변고도상관（｜r｜＞0．7，P＜0．05）。결론 UA가억제CDDP소치TRPV1적고표체，유효강저CD_DP적이독성，개선은공능。
Objective To investigate the effects of ursolic acid (UA) on cisplatin (CDDP)-induced expres_sion of transient receptor potential vanilloid 1 (TRPV1) in mouse cochlea .Methods Sixty BALB/c mice were ran_domly divided into 4 groups (15 mice in each group) and received introperitoneal injection once daily for 5 days:Control group (normol saline) ,UA group (80 mg/kg/day) ,CDDP group (4 .5 mg/kg/day) ,and CDPP (4 .5 mg/kg/day) plus UA group (80 mg/kg/day) .The expression of TRPV1 in mouse cochlea was determined by immuno_histochemistry ,microscope image analysis and western blot ,and auditory thresholds were evaluated by auditory brainstem response (ABR) measurement .ResuIts The expression of cochlea TRPV1 and ABR threshold shift was significantly increased in the mice treated with CDDP (P< 0 .05) ,as compared with control mice .These effects were prevented by UA treatment (all P<0 .05) .Furthermore ,a linear relationship analysis revealed that the ex_pression of cochlea TRPV1 was significantly correlated with ABR threshold shift(|r|>0 .7 , P<0 .05) .ConcIusion UA effectively attenuated CDDP -induced ototoxicity and improved auditory function through inhibition of TR_PV1 .