CAJ | 학술논문

建立了一种测定蓝藻细胞培养液中蔗糖和甘油葡糖苷的高效阴离子交换色谱积分脉冲安培检测法。在最佳的分离条件下，蔗糖和甘油葡糖苷的质量浓度在0.1～50.0 mg／L范围内与色谱峰面积线性良好，线性相关系数r2>0.9999。蔗糖和甘油葡糖苷的最低检测限分别是0.15 mg／L和0.03 mg／L，测定结果的相对标准偏差小于2%（n=8）。该方法样品处理简单，无基体干扰，测定的准确度，灵敏度高，可应用于蓝藻培养液中蔗糖和甘油葡糖苷的测定。
건립료일충측정람조세포배양액중자당화감유포당감적고효음리자교환색보적분맥충안배검측법。재최가적분리조건하，자당화감유포당감적질량농도재0.1～50.0 mg／L범위내여색보봉면적선성량호，선성상관계수r2>0.9999。자당화감유포당감적최저검측한분별시0.15 mg／L화0.03 mg／L，측정결과적상대표준편차소우2%（n=8）。해방법양품처리간단，무기체간우，측정적준학도，령민도고，가응용우람조배양액중자당화감유포당감적측정。
A high performance anion-exchange chromatography with pulsed amperometric detection was developed for the determination of sucrose and glucosylglycerol in cyanobacterial cells. Under the optimized conditions,the concentration of sucrose and glucosylglycerol showed good linearity (r2>0.999 9) with the chromatographic peak area in the range of 0.1–50.0 mg/L. The limit of detection(LOD) was 0.15 mg/L for sucrose and 0.03 mg/L for glucosylglycerol. RSDs of the detection results were less than 2% (n=8). The result showed that the method was convenient and accurate for the determination of sucrose and glucosylglycerol in cyanobacterial cells.