化学分析计量
化學分析計量
화학분석계량
CHEMICAL ANALYSIS AND METERAGE
2014年
z1期
13-15
,共3页
离子排斥色谱%秸秆发酵液%有机酸%糖%醇
離子排斥色譜%秸稈髮酵液%有機痠%糖%醇
리자배척색보%갈간발효액%유궤산%당%순
ion-exclusion chromatography%straw fermentation%organic acids%sugars%alcohol
建立了离子排斥色谱法同时分析生物质燃料乙醇发酵液中糖、酸和醇的方法。使用Bio-Rad Aminex HPX–87H离子排斥色谱柱，以5 mmol／L H2SO4为流动相，柱温为65℃，流量为0.6 mL／min，用示差折光检测器对玉米秸秆发酵液中的纤维二糖、葡萄糖、木糖、阿拉伯糖、甲酸、乙酸、丁酸、乙醇进行定量分析。方法的加标回收率为97.5%～107.4%，相对标准偏差为0.27%～2.19%(n=6)，线性相关系数均大于0.999。该方法适用于生物质发酵制乙醇过程中发酵糖、有机酸和乙醇的测定。
建立瞭離子排斥色譜法同時分析生物質燃料乙醇髮酵液中糖、痠和醇的方法。使用Bio-Rad Aminex HPX–87H離子排斥色譜柱，以5 mmol／L H2SO4為流動相，柱溫為65℃，流量為0.6 mL／min，用示差摺光檢測器對玉米秸稈髮酵液中的纖維二糖、葡萄糖、木糖、阿拉伯糖、甲痠、乙痠、丁痠、乙醇進行定量分析。方法的加標迴收率為97.5%～107.4%，相對標準偏差為0.27%～2.19%(n=6)，線性相關繫數均大于0.999。該方法適用于生物質髮酵製乙醇過程中髮酵糖、有機痠和乙醇的測定。
건립료리자배척색보법동시분석생물질연료을순발효액중당、산화순적방법。사용Bio-Rad Aminex HPX–87H리자배척색보주，이5 mmol／L H2SO4위류동상，주온위65℃，류량위0.6 mL／min，용시차절광검측기대옥미갈간발효액중적섬유이당、포도당、목당、아랍백당、갑산、을산、정산、을순진행정량분석。방법적가표회수솔위97.5%～107.4%，상대표준편차위0.27%～2.19%(n=6)，선성상관계수균대우0.999。해방법괄용우생물질발효제을순과정중발효당、유궤산화을순적측정。
An ion-exclusion chromatography method for simultaneous determination of organic acids,sugars and alcohol in straw fermentation was investigated. An ion-exclusion column (Bio-Rad Aminex HPX–87H) was used at 65℃,5 mmol/L H2SO4 solution with theflow rate of 0.6 mL/min. 8 compounds of cellobiose,glucose,xylose,arabinose,formic acid,acetic acid,butyric acid and ethanol were detected by refractive index detector. The linear correlation coefficients were above 0.999. Under the optimized conditions,the recoveries were in the range of 97.5%–107.4% with the relative standard deviations of 0.27%–2.19%(n=6). This method is suitable for the analysis of organic acids,sugars and alcohols in straw fermentation.
