CAJ | 학술논문

目的：体外建立顺铂（ DDP）诱导的人肝癌耐药细胞株（ HepG2／DDP），研究其生物学特性。方法以采用药物大剂量冲击结合低剂量持续诱导方法诱导HepG2细胞株，建立人肝癌顺铂耐药细胞株HepG2／DDP；MTT法检测顺铂对HepG2和HepG2／DDP的半数抑制浓度（IC50）和耐药系数（RI），绘制细胞生长曲线及计算细胞倍增时间，并用流式细胞仪（ FCM ）检测细胞周期。结果历时5个月成功建成HepG2／DDP细胞株，MTT法检测DDP对HepG2的IC50为1．35μg／ml，HepG2／DDP的IC50为23．35μg／ml，RI达17生长曲线表明其增殖速度明显低于亲本HepG2人肝癌细胞，且倍增时间增加；细胞周期分析发现相对于亲本HepG2细胞，G0／G1期细胞减少、S期与G2／M期细胞增多。结论成功建立稳定耐药细胞株HepG2／DDP。
목적：체외건립순박（ DDP）유도적인간암내약세포주（ HepG2／DDP），연구기생물학특성。방법이채용약물대제량충격결합저제량지속유도방법유도HepG2세포주，건립인간암순박내약세포주HepG2／DDP；MTT법검측순박대HepG2화HepG2／DDP적반수억제농도（IC50）화내약계수（RI），회제세포생장곡선급계산세포배증시간，병용류식세포의（ FCM ）검측세포주기。결과력시5개월성공건성HepG2／DDP세포주，MTT법검측DDP대HepG2적IC50위1．35μg／ml，HepG2／DDP적IC50위23．35μg／ml，RI체17생장곡선표명기증식속도명현저우친본HepG2인간암세포，차배증시간증가；세포주기분석발현상대우친본HepG2세포，G0／G1기세포감소、S기여G2／M기세포증다。결론성공건립은정내약세포주HepG2／DDP。
Objective To establish a drug-resistant hepatocellular carcinoma cell line and observe its biological characters .Methods The drug-resistant cell line HepG2 was induced using a concentration gradient of cisplatin (DDP) with exposed to intermittently.Drug sensitivity of HepG2 and HepG2/DDP were detected by MTT assay.The resistance index of the resistant cell line to DDP was determined by the methyl thiazolyl tetrazoliumassay ( MTT ) .Cell growth curve was painted by cell counting assay and the doubling time was accounted.Cycle distribution were measured by flow cytometry .Results The DDP-resistant cell line HepG2/DDP was established after 5 months and indicated drug-resistance to DDP, the drug resistance index being 17 respectively.The growing rate of resistant cells was obviously slower than that of the parental cells .The distributing proportion of HepG 2/DDP cells in G0/G1 phase was less than that of the parental cells , whereas the percentages of cells in G 2/M and S phase were significantly increased in HepG 2/DDP in comparison with those in HepG2.Conclusions A reliable drug-resistant human hepatoma cell line HepG 2/DDP is successfully established.