CAJ | 학술논문

目的：探讨蚕蛹水解氨基酸对HepG2．2．15（人肝癌细胞株）在体外的抑制作用。方法运用MTT比色法测定蚕蛹水解氨基酸对人QSG-7701（正常肝脏细胞株）的毒性后，计算蚕蛹水解氨基酸的安全浓度。将蚕蛹水解氨基酸溶液分别配制成0．001 mg／ml、0．01 mg／ml、0．1 mg／ml、1 mg／ml和10 mg／ml的浓度，与HepG2．2．15细胞共同培养，实验同时设置空白对照和细胞对照组。每组设置4个复孔，每孔加入100μl的5×104个／ml的细胞悬液，于培养的24、48、72小时后，用MTT比色法测定OD值，评定蚕蛹水解氨基酸对HepG2．2．15细胞株的抑制作用。结果蚕蛹水解氨基酸的最大无毒浓度（TC0）为12 mg／ml。梯度浓度的蚕蛹水解氨基酸与HepG2．2．15细胞共同培养24、48和72 h后，与细胞对照组相比，OD值的差异有显著性（P＜0．05），并有时间和浓度的线性关系。结论蚕蛹水解氨基酸对人肝癌细胞HepG2．2．15具有显著的抑制作用，且毒性较低。
목적：탐토잠용수해안기산대HepG2．2．15（인간암세포주）재체외적억제작용。방법운용MTT비색법측정잠용수해안기산대인QSG-7701（정상간장세포주）적독성후，계산잠용수해안기산적안전농도。장잠용수해안기산용액분별배제성0．001 mg／ml、0．01 mg／ml、0．1 mg／ml、1 mg／ml화10 mg／ml적농도，여HepG2．2．15세포공동배양，실험동시설치공백대조화세포대조조。매조설치4개복공，매공가입100μl적5×104개／ml적세포현액，우배양적24、48、72소시후，용MTT비색법측정OD치，평정잠용수해안기산대HepG2．2．15세포주적억제작용。결과잠용수해안기산적최대무독농도（TC0）위12 mg／ml。제도농도적잠용수해안기산여HepG2．2．15세포공동배양24、48화72 h후，여세포대조조상비，OD치적차이유현저성（P＜0．05），병유시간화농도적선성관계。결론잠용수해안기산대인간암세포HepG2．2．15구유현저적억제작용，차독성교저。
Objective To discusses the inhibition effect of hydrolytic amino acid of silkworm chrysalis (HAASC) to the HepG2.2.15.Methods The safe concentration of HAASC were obtained by MTT way to detect the cell toxicity of the HAASC to QSG-7701.And then, different concentration (0.001 mg/ml, 0.01 mg/ml, 0.1 mg/ml, 1 mg/ml and 10 mg/ml) of HAASC were added to every hole respectively , and blank control group ,cell control group were sent in the meantime .Each group was set up four holes .Each group was joined 100μl cell suspension with 5 ×104 /ml.which to raise 24, 48 and 72h.At last, estimating the restraining effect of HAASC to HepG2.2.15 by MTT ways to detecting OD value .Results The TC0 of HAASC was 12mg/ml.The OD value of different concentration of HAASC were different after 24,48 and 72h, later, comparing to cell control, and has significance (P <0.05), which had cable sex with time and concentration .Conclusions Hydrolytic amino acid of silkworm chrysalis has a significant inhibitory effect on the human hepatic cancer cells HepG2.2.15 and has low toxicity .