CAJ | 학술논문

目的：探讨心力衰竭（心衰）小鼠心肌组织中4．1 R蛋白的表达及其与心功能的关系。方法：42只雄性健康小鼠随机分为心衰组（n＝22）和对照组（n＝20）。处死小鼠后迅速取出心肌组织，行HE染色，计算左室质量指数和心肌病理积分；并采用免疫组化染色及免疫荧光标记法检测心衰小鼠心肌组织4．1R蛋白的表达。结果：与对照组比较，心衰组左室质量指数和心肌病理积分升高（ t＝190．668， Z＝31．915， P均＜0．05）；心衰组心肌组织4．1R蛋白阳性细胞百分比高于对照组（t＝18．607，P＝0．001）。 Pearson直线相关分析显示，心衰组心肌组织4．1R蛋白阳性细胞百分比与左室质量指数呈正相关（r＝0．766，P＜0．001）。结论：心衰小鼠心肌组织中4．1R蛋白的表达增加，干预4．1R蛋白的表达可能对心衰的治疗及预后有一定的意义。
목적：탐토심력쇠갈（심쇠）소서심기조직중4．1 R단백적표체급기여심공능적관계。방법：42지웅성건강소서수궤분위심쇠조（n＝22）화대조조（n＝20）。처사소서후신속취출심기조직，행HE염색，계산좌실질량지수화심기병리적분；병채용면역조화염색급면역형광표기법검측심쇠소서심기조직4．1R단백적표체。결과：여대조조비교，심쇠조좌실질량지수화심기병리적분승고（ t＝190．668， Z＝31．915， P균＜0．05）；심쇠조심기조직4．1R단백양성세포백분비고우대조조（t＝18．607，P＝0．001）。 Pearson직선상관분석현시，심쇠조심기조직4．1R단백양성세포백분비여좌실질량지수정정상관（r＝0．766，P＜0．001）。결론：심쇠소서심기조직중4．1R단백적표체증가，간예4．1R단백적표체가능대심쇠적치료급예후유일정적의의。
Aim:To detect the expression of 4.1R in myocardial tissue from mice with heart failure (HF) and its rela-tionship with cardiac function .Methods: A total of 42 male mice were randomly allocated into HF group ( n=22 ) and control group (n=20).All the mice were killed and the myocardial tissue was prepared for HE staining .The left ventricu-lar mass index and the myocardial tissue pathological score were assessed .The expression of 4.1R in myocardial tissue was detected by immunohistochemistry and immunofluorescence .Results: Compared with control group , the left ventricular mass index and the myocardial tissue pathological score in the HF group increased (t=190.668,Z=31.915,P<0.05). The positive rate of 4.1R in the HF group was significantly higher than that in control group (t=18.607, P=0.001).In the HF group, the positive correlation was found between the left ventricular mass index and the positive rate of 4.1R in myocardial tissue(r=0.766,P<0.001).Conclusion:The expression of 4.1R in myocardial tissue from mice with HR is increased and intervention of the expression of 4.1R may be helpful in the treatment and prognosis of HR.