CAJ | 학술논문

目的：观察骨髓间充质干细胞（MSCs）在子宫内膜损伤小鼠子宫内膜中的定位，初步探讨MSCs移植用于小鼠子宫内膜损伤后修复的可行性。方法：采用热损伤的方法建立小鼠子宫内膜损伤模型，随机分为MSCs组和对照组，每组15只。将体外分离培养的雄性小鼠MSCs经尾静脉联合宫腔内局部注射植入MSCs组小鼠体内，对照组注射等体积的PBS。通过对移植后子宫内膜组织的SRY DNA原位杂交来定位源自雄性供体的细胞。结果：MSCs组小鼠损伤侧子宫内膜组织中有SRY阳性细胞的定植，对照组小鼠则未见SRY阳性细胞。结论：MSCs可定植于子宫内膜损伤小鼠的子宫内膜组织。
목적：관찰골수간충질간세포（MSCs）재자궁내막손상소서자궁내막중적정위，초보탐토MSCs이식용우소서자궁내막손상후수복적가행성。방법：채용열손상적방법건립소서자궁내막손상모형，수궤분위MSCs조화대조조，매조15지。장체외분리배양적웅성소서MSCs경미정맥연합궁강내국부주사식입MSCs조소서체내，대조조주사등체적적PBS。통과대이식후자궁내막조직적SRY DNA원위잡교래정위원자웅성공체적세포。결과：MSCs조소서손상측자궁내막조직중유SRY양성세포적정식，대조조소서칙미견SRY양성세포。결론：MSCs가정식우자궁내막손상소서적자궁내막조직。
Aim:To study the feasibility of bone marrow mesenchymal stem cells (MSCs) repairing murine uterine en-dometrial damage. Methods:Mouse uterine endometrial damage models were made by heat injury and randomly allocated into MSCs group and control group , 15 in each group. Bone marrow MSCs were transplanted into MSCs group by intrauter- ine and intravenous injection. The control group was injected with the same amount of PBS. In situ hybridization was used to observe the expression of SRY-positive cells in the uterine endometrial tissue. Results: It was shown that the MSCs group appeared colonization of the SRY-positive cells in the uterine endometrium tissue , while the control group did not. Conclusion:The bone marrow MSCs could locate in the mouse damaged uterine endometrial tissue.