CAJ | 학술논문

目的：通过检测自分泌运动因子受体（ AMFR）在急性髓细胞白血病（ AML）患儿骨髓单个核细胞中的表达情况，探讨其与AML化疗缓解率的关系。方法：采用RT－PCR及Western blot方法检测试验组（63例AML患儿）与对照组（50例非AML患儿）骨髓单个核细胞中AMFR mRNA及蛋白的表达水平，其中试验组包括AML初治患儿35例、完全缓解患儿18例、复发患儿10例，分析AMFR mRNA表达与缓解率的关系。结果：①AMFR mRNA及蛋白表达水平在对照组、缓解组、初治组及复发组中逐渐升高（F＝13．105、10．010，P均＜0．001），组间两两比较差异均有统计学意义（P均＜0．05）。②AMFR mRNA高表达患儿的缓解率为15％，低于AMFR mRNA低表达患儿的缓解率84％（χ2＝21．254，P＜0．001）。结论：AMFR在儿童AML中存在高表达，AMFR可作为化疗敏感性及预后判断的指标。
목적：통과검측자분비운동인자수체（ AMFR）재급성수세포백혈병（ AML）환인골수단개핵세포중적표체정황，탐토기여AML화료완해솔적관계。방법：채용RT－PCR급Western blot방법검측시험조（63례AML환인）여대조조（50례비AML환인）골수단개핵세포중AMFR mRNA급단백적표체수평，기중시험조포괄AML초치환인35례、완전완해환인18례、복발환인10례，분석AMFR mRNA표체여완해솔적관계。결과：①AMFR mRNA급단백표체수평재대조조、완해조、초치조급복발조중축점승고（F＝13．105、10．010，P균＜0．001），조간량량비교차이균유통계학의의（P균＜0．05）。②AMFR mRNA고표체환인적완해솔위15％，저우AMFR mRNA저표체환인적완해솔84％（χ2＝21．254，P＜0．001）。결론：AMFR재인동AML중존재고표체，AMFR가작위화료민감성급예후판단적지표。
Aim:To investigate the autocrine motility factor receptor ( AMFR) expression in childhood acute myeloge-nous leukemia ( AML) and the relationship between the expression and clinical remission rate .Methods: The levels of AMFR protein and mRNA in 63 cases of AML children ( including 35 cases diagnosed newly ,18 cases with complete remis-sion,and 10 cases suffered from the recurrence of AML ) and 50 controls were detected by RT-PCR and Western blot , and the relationship between the expression of AMFR mRNA and the remission rate of AML was analyzed .Results: The ex-pression level of AMFR mRNA and protein in the control group ,the untreated group , the complete remission group , and the relapse group increased significantly in turn (F=13.105, 10.010,P<0.001).There were significant differences between each 2 groups among the 4 groups(P<0.05).The remission rate of children with lower AMFR mRNA expression (84%) was significantly higher than those with higher AMFR mRNA expression (15%)(χ2 =21.254,P<0.001).Conclusion:There is an overexpression of AMFR in childhood AML .AMFR could be used as an important indicator for judging the prognosis and chemotherapy sensitivity of AML .