CAJ | 학술논문

目的：探讨葛根芩连汤对牙周炎合并动脉粥样硬化大鼠模型TNF－α水平的影响。方法：将40只健康雄性Wistar大鼠分为对照组（ N组）和模型组（ D组）， D组接受造模处理。造模成功后将D组分为盐水治疗组（ D1组）、甲硝唑治疗组（ D2组）、阿托伐他汀治疗组（ D3组）、甲硝唑联合阿托伐他汀治疗组（ D4组）、葛根芩连汤治疗组（ D5组），灌胃4周后，HE染色光镜下观察牙周组织和腹主动脉病理变化，采用免疫组化法检测TNF－α在牙周组织和腹主动脉中的表达，采用ELISA法检测血清TNF－α水平。结果：D2、D3、D4、D5组牙周组织和腹主动脉中TNF－α相对表达量均较D1组降低（P均＜0．05）。D2、D3、D4、D5组血清中TNF－α水平均较D1组降低（P＜0．05）。结论：葛根芩连汤能降低牙周炎合并动脉粥样硬化大鼠TNF－α水平，对牙周炎伴动脉粥样硬化疾病有良好的治疗效果。
목적：탐토갈근금련탕대아주염합병동맥죽양경화대서모형TNF－α수평적영향。방법：장40지건강웅성Wistar대서분위대조조（ N조）화모형조（ D조）， D조접수조모처리。조모성공후장D조분위염수치료조（ D1조）、갑초서치료조（ D2조）、아탁벌타정치료조（ D3조）、갑초서연합아탁벌타정치료조（ D4조）、갈근금련탕치료조（ D5조），관위4주후，HE염색광경하관찰아주조직화복주동맥병리변화，채용면역조화법검측TNF－α재아주조직화복주동맥중적표체，채용ELISA법검측혈청TNF－α수평。결과：D2、D3、D4、D5조아주조직화복주동맥중TNF－α상대표체량균교D1조강저（P균＜0．05）。D2、D3、D4、D5조혈청중TNF－α수평균교D1조강저（P＜0．05）。결론：갈근금련탕능강저아주염합병동맥죽양경화대서TNF－α수평，대아주염반동맥죽양경화질병유량호적치료효과。
Aim:To observe the effects of Gegenqinlian decoction on TNF-αlevel in rats with periodontitis and athero-sclerosis.Methods:A total of 40 healthy male Wistar rats were divided into normal control group ( N group) and model group( D group ). After successful modeling , D group was divided into saline-treated group ( D1 group ) , metronidazole treatment group(D2 group), atorvastatin treatment group(D3 group), metronidazole plus atorvastatin treatment group (D4 group) , and Gegenqinlian decoction treatment group ( D5 group). Immunohistochemical staining was used to detect TNF-αin periodontal tissue and abdominal aorta. ELISA was used to detect serum TNF-αlevel.Results: TNF-αlevel in D2, D3, D4, D5 group was lower compared with D1 group, and the difference was statistically significant (P<0.05).Conclu-sion:Gegenqinlian decoction could reduce TNF-αlevel in rats with periodontitis and atherosclerosis.