CAJ | 학술논문

目的：观察宫内感染肺损伤时脂肪型脂肪酸结合蛋白（FABP4）在早产大鼠支气管肺泡灌洗液（BALF）及肺组织中的表达，探讨其与新型支气管肺发育不良（ BPD）发病的关系。方法：定期受孕的SD大鼠分为LPS组和对照组，于孕15 d羊膜腔内注射LPS或生理盐水。2组动物均于出生后第1天（P1）、第4天（P4）和第7天（P7）各取8只，应用ELISA方法检测BALF中FABP4的含量，采用免疫组化法和RT－PCR技术检测肺组织中FABP4蛋白及mRNA的表达水平。结果：FABP4主要在肺泡巨噬细胞、血管内皮细胞及少量支气管上皮细胞表达。 LPS组早产大鼠BALF中FABP4含量、肺组织中FABP4蛋白及mRNA的相对表达量较对照组均升高，且与时间存在交互作用（P均＜0．05）。结论：宫内感染肺损伤时FABP4表达升高，可能是引起肺微血管病理性重塑及肺泡化进程受阻，进而导致新型BPD发生发展的重要因素。
목적：관찰궁내감염폐손상시지방형지방산결합단백（FABP4）재조산대서지기관폐포관세액（BALF）급폐조직중적표체，탐토기여신형지기관폐발육불량（ BPD）발병적관계。방법：정기수잉적SD대서분위LPS조화대조조，우잉15 d양막강내주사LPS혹생리염수。2조동물균우출생후제1천（P1）、제4천（P4）화제7천（P7）각취8지，응용ELISA방법검측BALF중FABP4적함량，채용면역조화법화RT－PCR기술검측폐조직중FABP4단백급mRNA적표체수평。결과：FABP4주요재폐포거서세포、혈관내피세포급소량지기관상피세포표체。 LPS조조산대서BALF중FABP4함량、폐조직중FABP4단백급mRNA적상대표체량교대조조균승고，차여시간존재교호작용（P균＜0．05）。결론：궁내감염폐손상시FABP4표체승고，가능시인기폐미혈관병이성중소급폐포화진정수조，진이도치신형BPD발생발전적중요인소。
Aim:To investigate the expression of fatty acid binding protein 4 ( FABP4) in bronchoalveolar lavage fluid ( BALF) and lung tissue of preterm rats exposed to intra-amniotic endotoxin and to elucidate the relationship between intra-uterine inflammatory exposure and the pathogenesis of bronchopulmonary dysplasia (BPD).Methods:Timed pregnant SD rats were randomly divided into two groups:control group and LPS group .LPS or normal saline were injected into the amni-otic cavity, respectively.The lungs from eight rats of each group aged postnatal day 1(P1), day 4(P4) and day 7(P7) were removed and the level of FABP 4 in BALF was measured by ELISA .The expressions of FABP 4 protein and mRNA in lung tissue were assessed by immunohistochemistry and RT-PCR.Results:FABP4 immunoreactivity was detected in alveo-lar macrophages , endothelial cells and epithelial cells in both groups .Compared with control group , the FABP4 level in BALF, FABP4 protein and mRNA levels in lung tissue of LPS group increased (P<0.05).Conclusion: Intra-amniotic endotoxin could change the expression of FABP 4, which may contribute to BPD .