郑州大学学报（医学版）
鄭州大學學報（醫學版）
정주대학학보（의학판）
JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES)
2015年
1期
41-44
,共4页
吴余%崔静%王虹%付琳琳%李航%田明振%冯思佳%杨璐%赵二趁%千新来%原志庆
吳餘%崔靜%王虹%付琳琳%李航%田明振%馮思佳%楊璐%趙二趁%韆新來%原誌慶
오여%최정%왕홍%부림림%리항%전명진%풍사가%양로%조이진%천신래%원지경
人乳头瘤病毒16%宫颈鳞状细胞癌%E6基因%粘蛋白抗原16
人乳頭瘤病毒16%宮頸鱗狀細胞癌%E6基因%粘蛋白抗原16
인유두류병독16%궁경린상세포암%E6기인%점단백항원16
human papillomavirus 16%cervical squamous cell carcinoma%E6 gene%mucin antigen 16
目的：探讨人乳头瘤病毒（HPV）16相关宫颈癌组织中粘蛋白抗原16（MUC16）与 E6表达的关系。方法：以HPV16 E6阳性和阴性的宫颈癌细胞系和宫颈鳞状细胞癌（简称鳞癌）组织（184例）为研究对象，采用West－ern blot和免疫组化方法检测HPV16 E6和MUC16的表达。结果：Western blot 结果显示：C－33A细胞中HPV16 E6无表达，CaSki细胞和SiHa细胞中可见HPV16 E6表达；C－33A细胞中MUC16的表达低于CaSki细胞和SiHa细胞（P＜0．05），CaSki细胞和SiHa细胞中MUC16的表达差异无统计学意义（P＞0．05）。免疫组化结果显示：HPV16 E6阳性的宫颈鳞癌组织中MUC16的表达高于HPV16 E6阴性的宫颈鳞癌组织（χ2＝43．670，P＜0．001）。结论：宫颈癌细胞系和鳞癌组织中MUC16与E6表达可能有关。
目的：探討人乳頭瘤病毒（HPV）16相關宮頸癌組織中粘蛋白抗原16（MUC16）與 E6錶達的關繫。方法：以HPV16 E6暘性和陰性的宮頸癌細胞繫和宮頸鱗狀細胞癌（簡稱鱗癌）組織（184例）為研究對象，採用West－ern blot和免疫組化方法檢測HPV16 E6和MUC16的錶達。結果：Western blot 結果顯示：C－33A細胞中HPV16 E6無錶達，CaSki細胞和SiHa細胞中可見HPV16 E6錶達；C－33A細胞中MUC16的錶達低于CaSki細胞和SiHa細胞（P＜0．05），CaSki細胞和SiHa細胞中MUC16的錶達差異無統計學意義（P＞0．05）。免疫組化結果顯示：HPV16 E6暘性的宮頸鱗癌組織中MUC16的錶達高于HPV16 E6陰性的宮頸鱗癌組織（χ2＝43．670，P＜0．001）。結論：宮頸癌細胞繫和鱗癌組織中MUC16與E6錶達可能有關。
목적：탐토인유두류병독（HPV）16상관궁경암조직중점단백항원16（MUC16）여 E6표체적관계。방법：이HPV16 E6양성화음성적궁경암세포계화궁경린상세포암（간칭린암）조직（184례）위연구대상，채용West－ern blot화면역조화방법검측HPV16 E6화MUC16적표체。결과：Western blot 결과현시：C－33A세포중HPV16 E6무표체，CaSki세포화SiHa세포중가견HPV16 E6표체；C－33A세포중MUC16적표체저우CaSki세포화SiHa세포（P＜0．05），CaSki세포화SiHa세포중MUC16적표체차이무통계학의의（P＞0．05）。면역조화결과현시：HPV16 E6양성적궁경린암조직중MUC16적표체고우HPV16 E6음성적궁경린암조직（χ2＝43．670，P＜0．001）。결론：궁경암세포계화린암조직중MUC16여E6표체가능유관。
Aim:To investigate the relationship of mucin antigen 16 ( MUC16 ) and E6 in human papillomavirus 16 (HPV16)-related cervical carcinoma tissue.Methods:HPV16 E6 positive and negative cervical carcinoma cell lines and 184 cases of cervical squamous cell carcinoma tissue were collected .The expressions of HPV16 E6 and MUC16 were detec-ted by Western blot and immunohistochemistry .Results:HPV16 E6 was not expressed in C-33A cells, but expressed in CaSki cells and SiHa cells .The expression of MUC16 in C-33A cells was significantly lower than those in CaSki cells and SiHa cells(P<0.05), whereas there was no significant difference in expression of MUC 16 between CaSki cells and SiHa cells(P>0.05).The expression of MUC16 in HPV16 E6 positive cervical squamous cell carcinoma tissue was significantly higher than that in HPV16 E6 negative cervical squamous cell carcinoma tissue (χ2 =43.670,P<0.001).Conclusion:The expression of MUC16 is correlated with E6 expression in cervical carcinoma cell lines and cervical squamous cell carci -noma tissue .
