郑州大学学报（医学版）
鄭州大學學報（醫學版）
정주대학학보（의학판）
JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES)
2015年
1期
29-32
,共4页
王虹%李娜%付琳琳%吴余%田明振%李航%冯思佳%杨璐%赵二趁%千新来
王虹%李娜%付琳琳%吳餘%田明振%李航%馮思佳%楊璐%趙二趁%韆新來
왕홍%리나%부림림%오여%전명진%리항%풍사가%양로%조이진%천신래
人乳头瘤病毒%宫颈癌%E7%肾细胞癌下调基因1
人乳頭瘤病毒%宮頸癌%E7%腎細胞癌下調基因1
인유두류병독%궁경암%E7%신세포암하조기인1
human papillomavirus%cervical cancer%E7%down-regulated in renal cell carcinoma 1
目的：研究人乳头瘤病毒16（HPV16）相关宫颈癌细胞系和组织中E7和肾细胞癌下调基因1（DRR1）表达的关系。方法：以HPV16 E7阳性的宫颈癌CaSki和SiHa细胞、HPV16 E7阴性的宫颈癌C－33A细胞及184例宫颈鳞状细胞癌组织为研究对象，采用Western blot方法和免疫组化染色方法检测E7和DRR1蛋白的表达，分析E7与DRR1蛋白表达的关系。结果：Western blot结果显示，C－33A细胞中HPV16 E7无表达，DRR1蛋白的表达显著高于CaSki细胞和SiHa细胞（F＝454．982，P＜0．001）；CaSki细胞和SiHa细胞表达HPV16 E7，二者DRR1蛋白的表达差异无统计学意义（ P＞0．05）。免疫组化染色结果显示，HPV16 E7阳性宫颈鳞状细胞癌组织141例，其中DRR1蛋白阳性34例；HPV16 E7阴性宫颈鳞状细胞癌组织43例，其中DRR1蛋白阳性32例；HPV16 E7阳性组织中DRR1蛋白的表达显著低于HPV16 E7阴性组织（χ2＝36．250，P＜0．001）。结论：DRR1可能是HPV16 E7作用的潜在靶基因。
目的：研究人乳頭瘤病毒16（HPV16）相關宮頸癌細胞繫和組織中E7和腎細胞癌下調基因1（DRR1）錶達的關繫。方法：以HPV16 E7暘性的宮頸癌CaSki和SiHa細胞、HPV16 E7陰性的宮頸癌C－33A細胞及184例宮頸鱗狀細胞癌組織為研究對象，採用Western blot方法和免疫組化染色方法檢測E7和DRR1蛋白的錶達，分析E7與DRR1蛋白錶達的關繫。結果：Western blot結果顯示，C－33A細胞中HPV16 E7無錶達，DRR1蛋白的錶達顯著高于CaSki細胞和SiHa細胞（F＝454．982，P＜0．001）；CaSki細胞和SiHa細胞錶達HPV16 E7，二者DRR1蛋白的錶達差異無統計學意義（ P＞0．05）。免疫組化染色結果顯示，HPV16 E7暘性宮頸鱗狀細胞癌組織141例，其中DRR1蛋白暘性34例；HPV16 E7陰性宮頸鱗狀細胞癌組織43例，其中DRR1蛋白暘性32例；HPV16 E7暘性組織中DRR1蛋白的錶達顯著低于HPV16 E7陰性組織（χ2＝36．250，P＜0．001）。結論：DRR1可能是HPV16 E7作用的潛在靶基因。
목적：연구인유두류병독16（HPV16）상관궁경암세포계화조직중E7화신세포암하조기인1（DRR1）표체적관계。방법：이HPV16 E7양성적궁경암CaSki화SiHa세포、HPV16 E7음성적궁경암C－33A세포급184례궁경린상세포암조직위연구대상，채용Western blot방법화면역조화염색방법검측E7화DRR1단백적표체，분석E7여DRR1단백표체적관계。결과：Western blot결과현시，C－33A세포중HPV16 E7무표체，DRR1단백적표체현저고우CaSki세포화SiHa세포（F＝454．982，P＜0．001）；CaSki세포화SiHa세포표체HPV16 E7，이자DRR1단백적표체차이무통계학의의（ P＞0．05）。면역조화염색결과현시，HPV16 E7양성궁경린상세포암조직141례，기중DRR1단백양성34례；HPV16 E7음성궁경린상세포암조직43례，기중DRR1단백양성32례；HPV16 E7양성조직중DRR1단백적표체현저저우HPV16 E7음성조직（χ2＝36．250，P＜0．001）。결론：DRR1가능시HPV16 E7작용적잠재파기인。
Aim:To study the relationship between human papilloma virus type 16 ( HPV16 ) E7 and down-regulated in renal cell carcinoma 1 ( DRR1 ) in HPV16 related cervical cancer cell line and tissue .Methods: CaSki cells , SiHa cells, C-33A cells and 184 cases of cervical squamous cell carcinoma tissue were objected to detect the expressions of E 7 and DRR1 by Western blot and immunohistochemistry .Results:The results of Western blot showed that HPV 16 E7 did not expressed in C-33A cells, but expressed in SiHa and CaSki cells .The expression of DRR1 in C-33A cells was significantly higher than those in SiHa and CaSki cells (F=454.982,P<0.001).The expression of DRR1 in SiHa cells and CaSki cells had no significant difference (P>0.05).The results of immunohistochemistry showed that there were 141 cases with HPV16 E7 positive expression, out of which 34 cases were DRR1 positive;there were 32 cases with DRR1 positive expres-sion, out of which 43 cases with HPV16 E7 negative expression .The expression of DRR1 in HPV16 E7 positive cervical squamous cell carcinoma tissue were significantly lower than that in HPV 16 E7 negative cervical squamous cell carcinoma tissue(χ2 =36.250,P<0.001).Conclusion:DRR1 may be the targeted gene of HPV16 E7.
