CAJ | 학술논문

从患典型鳃腺炎病中华鳖肝脏和腹水处进行细菌接种，分离获得一株细菌。综合梅里埃细菌生化鉴定系统和16S rRNA序列进化分析，判断所分离菌株为奇异变形杆菌（SX37）。动物回归试验显示，该分离菌株能够引起幼鳖发病死亡，并且能够从试验幼鳖体内再次分离到相同的病原菌。但临床症状与病理解剖结果与自然发病症状不一致，显示该菌株不是中华鳖鳃腺炎病病原，而是继发病原。分离株对小鼠的半数致死量为107．83，显示该菌株具有较强的致病性。
종환전형새선염병중화별간장화복수처진행세균접충，분리획득일주세균。종합매리애세균생화감정계통화16S rRNA서렬진화분석，판단소분리균주위기이변형간균（SX37）。동물회귀시험현시，해분리균주능구인기유별발병사망，병차능구종시험유별체내재차분리도상동적병원균。단림상증상여병리해부결과여자연발병증상불일치，현시해균주불시중화별새선염병병원，이시계발병원。분리주대소서적반수치사량위107．83，현시해균주구유교강적치병성。
One bacterium was isolated from liver and ascites of diseased Chinese soft‐shelled turtle (Pelo‐discus sinensis ) ,outbreaked in a large‐scale breeding farm in Shangyu ,and identified as Proteus mirabilis , named SX37 through API ID32E by 16S rRNA sequencing , and biochemical reactions . Healthy soft‐shelled turtle was experimentally infected with the bacterial suspension ,and then the same bacterium was recovered with different clinic sydromes .Isolate SX43 demonstrated virulence potential in mouse models , with the median lethal dose (LD50 ) of 1 × 107 .83 cfu/mL in mice .