CAJ | 학술논문

以浙江省6个异育银鲫规模化养殖场为监控点，于2013年6—7月与9—10月分别对每个养殖场进行采样与检测，所采样品均为表观健康个体。通过细菌分离、鉴定及毒力基因测定，对其肝、脾、肾携带细菌进行多样性分析。12个批次共检出7种细菌，均为人、兽、鱼共患病原菌。气单胞菌的检出率占据首位，气溶素、细胞毒性肠毒素、不耐热细胞紧张性肠毒素、耐热性细胞紧张性肠毒素为其主要毒力基因型，提示其可能具有致病性。鲁氏不动杆菌、保科爱德华菌、类志贺邻单胞菌、腐败希瓦氏菌、嗜麦芽窄食单胞菌、大肠埃希氏菌的检出率则在各批次间存在较大差异。异育银鲫携带的细菌种类及致病性气单胞菌的高携带率对水产养殖与公共卫生造成极大的隐患。研究结果为建立水产养殖细菌性病原（尤其是气单胞菌）监测体系提供参考依据。
이절강성6개이육은즉규모화양식장위감공점，우2013년6—7월여9—10월분별대매개양식장진행채양여검측，소채양품균위표관건강개체。통과세균분리、감정급독력기인측정，대기간、비、신휴대세균진행다양성분석。12개비차공검출7충세균，균위인、수、어공환병원균。기단포균적검출솔점거수위，기용소、세포독성장독소、불내열세포긴장성장독소、내열성세포긴장성장독소위기주요독력기인형，제시기가능구유치병성。로씨불동간균、보과애덕화균、류지하린단포균、부패희와씨균、기맥아착식단포균、대장애희씨균적검출솔칙재각비차간존재교대차이。이육은즉휴대적세균충류급치병성기단포균적고휴대솔대수산양식여공공위생조성겁대적은환。연구결과위건립수산양식세균성병원（우기시기단포균）감측체계제공삼고의거。
An investigation on the diversity of bacterial organisms in hepatopanueas ,spleen and kidney of healthy hybridized crussian carp (Carassius auratus gibelio) was carried out in six large‐scale breeding farms in Zhejiang province during June —July and September—October in 2013 .From a total of 12 bat‐ches ,seven opportunistic bacterial species capable of affecting humans ,animals and fishes were identified . A eromonas spp .predominated both in June—July and September—October batches and aerA +act+alt‐act‐was the major virulence‐gene pattern ,indicating that the Aeromonas isolates exhibited potential pathogenic inclination .On the contrary ,Acinetobacter lwof fii ,Edwardsiella hoshinae ,Plesiomonas shigelloides , Shewanella putref aciens , Stenotrophomonas maltophilia and Escherichia coli showed varying isolation rates between June—July and September—October batches .Bacterial species composition and high carry‐ing‐rate of pathogenic A eromonas in hybridized prussian carp posed significant risks to aquaculture industry and public health .It is necessary to develop the surveillance system of potential bacterial pathogens ,espe‐cially A eromonas spp .,in hybridized crussian carp ,and to clarify the pathogenic mechanism to establish comprehensive prevention and control schemes .