水产科学
水產科學
수산과학
FISHERIES SCIENCE
2014年
12期
807-813
,共7页
副溶血性弧菌%拮抗菌%拮抗条件%枯草芽孢杆菌
副溶血性弧菌%拮抗菌%拮抗條件%枯草芽孢桿菌
부용혈성호균%길항균%길항조건%고초아포간균
Vibrio parahaemolyticus%antagonistic bacterium%antagonistic condition%Bacillus subtilis
对分别分离自菜园土壤、大蒜植株、大豆植株、蔓花生植株、淡水湖淤泥、仙人掌植株、芹菜植株、细菌型豆豉和日本纳豆的可产抗大肠杆菌活性物质的42株细菌进行筛选，获得1株对副溶血性弧菌具有较强拮抗作用的菌株JNT02，其发酵液对副溶血性弧菌的抑菌圈直径为（20．87±0．83） mm ；经16S rDNA序列分析和系统发育分析，菌株JNT02与枯草芽孢杆菌位于同一簇群，同源性达100％，初步鉴定为枯草芽孢杆菌；枯草芽孢杆菌JNT02在改良兰迪培养基中24 h可完全杀灭共培养的副溶血性弧菌，并且种子液带入的抗菌物质对其拮抗作用有累积效应，在氯化钠碱性蛋白胨水培养基中枯草芽孢杆菌JNT02对副溶血性弧菌却只有一定抑制作用，在人工海水中没有拮抗作用；改良兰迪培养基有利于枯草芽孢杆菌JNT02产抗菌物质。结果表明，枯草芽孢杆菌JNT02对副溶血性弧菌的拮抗作用主要依赖于其代谢产生的抗菌活性物质，取决于环境条件是否有利于其产生抗菌活性物质，而并非是否有利于其生长。
對分彆分離自菜園土壤、大蒜植株、大豆植株、蔓花生植株、淡水湖淤泥、仙人掌植株、芹菜植株、細菌型豆豉和日本納豆的可產抗大腸桿菌活性物質的42株細菌進行篩選，穫得1株對副溶血性弧菌具有較彊拮抗作用的菌株JNT02，其髮酵液對副溶血性弧菌的抑菌圈直徑為（20．87±0．83） mm ；經16S rDNA序列分析和繫統髮育分析，菌株JNT02與枯草芽孢桿菌位于同一簇群，同源性達100％，初步鑒定為枯草芽孢桿菌；枯草芽孢桿菌JNT02在改良蘭迪培養基中24 h可完全殺滅共培養的副溶血性弧菌，併且種子液帶入的抗菌物質對其拮抗作用有纍積效應，在氯化鈉堿性蛋白胨水培養基中枯草芽孢桿菌JNT02對副溶血性弧菌卻隻有一定抑製作用，在人工海水中沒有拮抗作用；改良蘭迪培養基有利于枯草芽孢桿菌JNT02產抗菌物質。結果錶明，枯草芽孢桿菌JNT02對副溶血性弧菌的拮抗作用主要依賴于其代謝產生的抗菌活性物質，取決于環境條件是否有利于其產生抗菌活性物質，而併非是否有利于其生長。
대분별분리자채완토양、대산식주、대두식주、만화생식주、담수호어니、선인장식주、근채식주、세균형두시화일본납두적가산항대장간균활성물질적42주세균진행사선，획득1주대부용혈성호균구유교강길항작용적균주JNT02，기발효액대부용혈성호균적억균권직경위（20．87±0．83） mm ；경16S rDNA서렬분석화계통발육분석，균주JNT02여고초아포간균위우동일족군，동원성체100％，초보감정위고초아포간균；고초아포간균JNT02재개량란적배양기중24 h가완전살멸공배양적부용혈성호균，병차충자액대입적항균물질대기길항작용유루적효응，재록화납감성단백동수배양기중고초아포간균JNT02대부용혈성호균각지유일정억제작용，재인공해수중몰유길항작용；개량란적배양기유리우고초아포간균JNT02산항균물질。결과표명，고초아포간균JNT02대부용혈성호균적길항작용주요의뢰우기대사산생적항균활성물질，취결우배경조건시부유리우기산생항균활성물질，이병비시부유리우기생장。
The strain JNT02 ,which showed significantly inhibitory effects on the growth of Vibrio parah‐aemolyticus with (20 .87 ± 0 .83) mm diameter of antibiotic circle fermented broth for antimicrobial activity assay ,was screened from 42 bacterial strains isolated from vegetable garden soils ,garlic plants ,soybean plants ,vine peanut plants ,freshwater lake silts ,cactus plants ,celery plants ,bacterial soybean lobster sauce and Japanese natto especially producing antibiotic substances against Escherichia coli .The strain JNT02 was classified into Bacillus subtilis base on 16S rDNA sequence and phylogenic analysis .In the im‐proved Landy media ,B .subtilis JNT02 completely killed the co‐cultured V .parahaemolyticus in 24 h , and the enhancement of antagonistic effects of the antimicrobial substances brought from the B .subtilis JNT02 seed broth were also demonstrated .However ,B .subtilis JNT02 showed weak inhibitory effects a‐gainst the growth of V . parahaemolyticus in alkaline‐peptone‐water media and no antagonistic effects in artificial seawater .The improved Landy media were propitious to the antimicrobial substance production of B .subtilis JNT02 .The findings indicated that the antagonistic action of B .subtilis JNT02 against V . parahaemolyticus was via the antimicrobial substance producing ,and depended on the conditions of the an‐timicrobial substance production ,in stead of the growth of B .subtilis JNT02 .
