CAJ | 학술논문

为了解我国3个地区（广东省、广西省和海南省）罗非鱼源无乳链球菌临床株的遗传背景及进化关系，对2010—2011年间采集分离的128株链球菌，采用Bio‐MerieuxVITEK进行生理生化分析和16S rRNA法鉴定菌株类型，并运用多位点序列分型技术，确定其等位基因谱型及菌株序列型。试验结果表明，通过对分离菌株的生理生化及16S rRNA 基因进行分析，确认有104株为无乳链球菌，这104株分属1个已知克隆系ST‐7和2个未知克隆系NST‐1和NST‐2，其中NST‐1克隆系所占比列最多，占88．5％（92／104）。本研究结果揭示了我国南方3个地区无乳链球菌分离株呈多克隆系并存，以新型NST‐1克隆系为主，应加强对新克隆系的病原学监测。应用MLST 分子分型技术，初步揭示了我国南方3个地区罗非鱼源无乳链球菌的遗传进化关系。
위료해아국3개지구（광동성、광서성화해남성）라비어원무유련구균림상주적유전배경급진화관계，대2010—2011년간채집분리적128주련구균，채용Bio‐MerieuxVITEK진행생리생화분석화16S rRNA법감정균주류형，병운용다위점서렬분형기술，학정기등위기인보형급균주서렬형。시험결과표명，통과대분리균주적생리생화급16S rRNA 기인진행분석，학인유104주위무유련구균，저104주분속1개이지극륭계ST‐7화2개미지극륭계NST‐1화NST‐2，기중NST‐1극륭계소점비렬최다，점88．5％（92／104）。본연구결과게시료아국남방3개지구무유련구균분리주정다극륭계병존，이신형NST‐1극륭계위주，응가강대신극륭계적병원학감측。응용MLST 분자분형기술，초보게시료아국남방3개지구라비어원무유련구균적유전진화관계。
Identification was carried out in 128 strains of Streptococcus isolated from tilapia in southern China (Guangdong ,Guangxi and Hainan provinces ) from 2010 to 2011 by Bio‐MerieuxVITEK and 16S rRNA sequence analysis and 7 housekeeping genes were seguenced in 104 strains of S .agalactiae by Mul‐tilocus Sequence Typing (MLST ) technique to evaluate the genetic diversity of S . agalactiae .Results were analyzed and compared with sequence information in PubMLST databases to determine a strain allelic profile and assigned a sequence type (ST ) .It was found that total 104 strains were within 3 clone se‐quences :ST‐7 and 2 types of unidentified NST‐1 and NST‐2 ,NST‐1 having the maximal proportion of 88 .5% (92/104) .It was revealed that there were multiple clonal sequences of S .agalactiae isolate from tilapia in southern China with high pathogenetic and prevalent .It is suggested that S . agalactiae isolate from tilapia be intense surveillance efforts .The findings indicate that MLST technique is an ideal tool to reveal genetic evolution profile of S .agalactiae isolated from tilapia in southern China .