CAJ | 학술논문

目的：探讨Ku70和人端粒酶逆转录酶( hTERT)蛋白表达与胃癌发生发展的关系及两者之间的联系。方法应用免疫组织化学方法检测Ku70和hTERT蛋白在10例正常胃黏膜、15例胃癌前病变黏膜和20例胃癌组织中的表达。结果 Ku70蛋白阳性表达定位于正常胃黏膜和胃癌组织的细胞核，胃癌前病变黏膜的细胞浆，其阳性表达率分别为90‰、100‰和80‰；hTERT蛋白在正常胃黏膜几乎不表达，在胃癌前病变黏膜和胃癌组织中均定位于细胞浆，其阳性表达率分别为30‰、100‰和100‰。胃癌组织中Ku70和hTERT蛋白的表达强度明显高于正常胃黏膜和胃癌前病变黏膜。 Ku70和hTERT蛋白的表达显著相关。结论 Ku70和hTERT蛋白可能与胃癌的发生发展过程相关。
목적：탐토Ku70화인단립매역전록매( hTERT)단백표체여위암발생발전적관계급량자지간적련계。방법응용면역조직화학방법검측Ku70화hTERT단백재10례정상위점막、15례위암전병변점막화20례위암조직중적표체。결과 Ku70단백양성표체정위우정상위점막화위암조직적세포핵，위암전병변점막적세포장，기양성표체솔분별위90‰、100‰화80‰；hTERT단백재정상위점막궤호불표체，재위암전병변점막화위암조직중균정위우세포장，기양성표체솔분별위30‰、100‰화100‰。위암조직중Ku70화hTERT단백적표체강도명현고우정상위점막화위암전병변점막。 Ku70화hTERT단백적표체현저상관。결론 Ku70화hTERT단백가능여위암적발생발전과정상관。
Objective To explore the relationship of Ku70 and hTERT protein expressions in the progression of hu-man gastric carcinogenesis and their correlations. Methods Expressions of Ku70 and hTERT protein in 10 normal gas-tric mucosa,15 precancerous lesions and 20 gastric cancers were examined by immunohistochemistry. Results Ku70 was found in normal gastric mucosa with mild nuclear expression,in precancerous lesion with mild cytoplasmic expression and in GC with marked nuclear expression and its positive expression rates were 90‰,80‰ and 100‰ respectively. hTERT was found in cytoplasm and its positive expression rates in normal gastric mucosa,precancerous lesion and gastric cancer were respectively 20‰,100‰ and 100‰. The staining densities of Ku70 and hTERT protein in gastric cancer tissues were sig-nificantly higher than that in normal gastric mucosa and precancerous lesions. There was a significant correlation between the expressions of Ku70 and hTERT. Conclusion Ku70 and hTERT protein protein might play an important role in the pro-gression of human gastric carcinogenesis.