中南医学科学杂志
中南醫學科學雜誌
중남의학과학잡지
JOURNAL OF UNIVERSITY OF SOUTH CHINA(MEDICAL EDITION)
2015年
1期
21-25
,共5页
贺正希%唐慧岚%陈景飞%陈文琳%蒋炜峥%谢远杰
賀正希%唐慧嵐%陳景飛%陳文琳%蔣煒崢%謝遠傑
하정희%당혜람%진경비%진문림%장위쟁%사원걸
胃癌%葡萄糖调节蛋白78%分化%淋巴结转移%临床分期
胃癌%葡萄糖調節蛋白78%分化%淋巴結轉移%臨床分期
위암%포도당조절단백78%분화%림파결전이%림상분기
gastric carcinoma%glucose regulatory protein 78 KD%differentiation%lymph node metastasis%clinical stage
目的：检测葡萄糖调节蛋白78(GRP78)在胃癌组织中的表达,分析其临床病理学意义,探讨GRP78在胃癌发生发展中的作用。方法收集48例不同临床分期及不同分化程度胃腺癌及28例癌旁正常胃黏膜组织标本,分别应用RT-PCR、Western Blotting和免疫组化检测GRP78 mRNA和蛋白质的表达。结果与正常胃黏膜组织相比,胃癌组织中GRP78 mRNA及蛋白表达水平显著增加(P<0.05)。免疫组化检测GRP78阳性表达定位于胞浆,在正常胃粘膜组织中GRP78阳性表达率为10.7‰(3/28),高、中分化与低分化胃癌组织阳性表达率分别为59.4‰(19/32)和93.8‰(15/16)；直径≥5 cm的胃癌组织中GRP78阳性表达率(88.9‰,16/18)高于直径<5 cm的胃癌组织(60‰,18/30),Ⅲ、Ⅳ期(TNM分期)病例胃癌组织中GRP78阳性表达率(90.5‰,19/21)高于Ⅰ、Ⅱ期病例(55.6‰,15/27)；5年内病情复发或者死亡病例胃癌组织中GRP78阳性表达率(82.8‰,24/29)高于5年内病情无复发病例(52.6‰,10/19)；淋巴结转移胃癌组织中GRP78阳性表达率(96.2‰,25/26)明显高于非淋巴结转移胃癌组织(40.9‰,9/22),各组间差异均具有统计学意义(P<0.05)。结论 GRP78在胃癌组织中高表达,其表达水平与胃癌肿瘤大小、分化程度、淋巴结转移、临床分期及预后密切相关。
目的：檢測葡萄糖調節蛋白78(GRP78)在胃癌組織中的錶達,分析其臨床病理學意義,探討GRP78在胃癌髮生髮展中的作用。方法收集48例不同臨床分期及不同分化程度胃腺癌及28例癌徬正常胃黏膜組織標本,分彆應用RT-PCR、Western Blotting和免疫組化檢測GRP78 mRNA和蛋白質的錶達。結果與正常胃黏膜組織相比,胃癌組織中GRP78 mRNA及蛋白錶達水平顯著增加(P<0.05)。免疫組化檢測GRP78暘性錶達定位于胞漿,在正常胃粘膜組織中GRP78暘性錶達率為10.7‰(3/28),高、中分化與低分化胃癌組織暘性錶達率分彆為59.4‰(19/32)和93.8‰(15/16)；直徑≥5 cm的胃癌組織中GRP78暘性錶達率(88.9‰,16/18)高于直徑<5 cm的胃癌組織(60‰,18/30),Ⅲ、Ⅳ期(TNM分期)病例胃癌組織中GRP78暘性錶達率(90.5‰,19/21)高于Ⅰ、Ⅱ期病例(55.6‰,15/27)；5年內病情複髮或者死亡病例胃癌組織中GRP78暘性錶達率(82.8‰,24/29)高于5年內病情無複髮病例(52.6‰,10/19)；淋巴結轉移胃癌組織中GRP78暘性錶達率(96.2‰,25/26)明顯高于非淋巴結轉移胃癌組織(40.9‰,9/22),各組間差異均具有統計學意義(P<0.05)。結論 GRP78在胃癌組織中高錶達,其錶達水平與胃癌腫瘤大小、分化程度、淋巴結轉移、臨床分期及預後密切相關。
목적：검측포도당조절단백78(GRP78)재위암조직중적표체,분석기림상병이학의의,탐토GRP78재위암발생발전중적작용。방법수집48례불동림상분기급불동분화정도위선암급28례암방정상위점막조직표본,분별응용RT-PCR、Western Blotting화면역조화검측GRP78 mRNA화단백질적표체。결과여정상위점막조직상비,위암조직중GRP78 mRNA급단백표체수평현저증가(P<0.05)。면역조화검측GRP78양성표체정위우포장,재정상위점막조직중GRP78양성표체솔위10.7‰(3/28),고、중분화여저분화위암조직양성표체솔분별위59.4‰(19/32)화93.8‰(15/16)；직경≥5 cm적위암조직중GRP78양성표체솔(88.9‰,16/18)고우직경<5 cm적위암조직(60‰,18/30),Ⅲ、Ⅳ기(TNM분기)병례위암조직중GRP78양성표체솔(90.5‰,19/21)고우Ⅰ、Ⅱ기병례(55.6‰,15/27)；5년내병정복발혹자사망병례위암조직중GRP78양성표체솔(82.8‰,24/29)고우5년내병정무복발병례(52.6‰,10/19)；림파결전이위암조직중GRP78양성표체솔(96.2‰,25/26)명현고우비림파결전이위암조직(40.9‰,9/22),각조간차이균구유통계학의의(P<0.05)。결론 GRP78재위암조직중고표체,기표체수평여위암종류대소、분화정도、림파결전이、림상분기급예후밀절상관。
Objective To explore the role of GRP78 in the development of gastric carcinoma by analysis of the ex-pression of GRP78 in gastric carcinoma tissue. Methods 48 cases of human gastric carcinoma tissue with different clinical period and differentiation degree and 28 cases of normal gastric tissue were selected. The mRNA and protein of GRP78 were detected by RT-PCR,Western blotting and immunohistochemistry,respectively. Results Compared with normal gastric epi-thelial tissue,the expression of GRP78 at mRNA and protein level in gastric carcinoma tissue increased significantly,which were showed by RT-PCR and Western Blotting(P<0. 05). GRP78 protein was mainly distributed in cytoplasm showed by im-munohistochemistry. The positive rate of GRP78 in normal gastric tissue,well or moderately-differentiated and poorly-differen-tiated gastric cancer tissue were 10. 7‰ (3/28),59. 4‰ (19/32) and 93. 8‰ (15/16),respectively. The positive rate of GRP78 in gastric carcinoma tissue with diameter ≥5 cm was 88. 9‰(16/18),atⅢ,Ⅳperiod in TNM staging was 90. 5‰(19/21),and with recurrence or death less than 5 years was 82. 8‰ (24/29),which were higher than that with diameter <5 cm(60‰,18/30),atⅠ,Ⅱperiod (55. 6‰,15/27),and with relapse-free in five years(52. 6‰,10/19),respectively. The positive rate of GRP78 in gastric carcinoma tissue with lymphatic metastasis was 96. 2‰ (25/26),which was much higher than that of without lymphatic metastasis (40. 9‰,9/22). Conclusion The expression of GRP78 in gastric cancer tissue increased,and GRP78 was involved in the development and progression of gastric cancer,which was highly related to tumor size,differentiation,lymph node metastasis,clinical stage and prognosis of gastric cancer.
