中南医学科学杂志
中南醫學科學雜誌
중남의학과학잡지
JOURNAL OF UNIVERSITY OF SOUTH CHINA(MEDICAL EDITION)
2015年
1期
39-41
,共3页
黄芩总黄酮苷元%保肝%四氯化碳%药理
黃芩總黃酮苷元%保肝%四氯化碳%藥理
황금총황동감원%보간%사록화탄%약리
Scutellaria Baicalensis Total Flavonoid%hepatoprotection%carbon tetrachloride%pharmacology
目的：探讨黄芩总黄酮苷元( SBTF)对肝脏的保护作用。方法将70只小鼠随机均分为7组(空白组、模型组、阴性对照组、联苯双酯阳性对照组及高、中、低剂量黄芩总苷元给药组)，每组10只，灌胃给药15天。高、中、低剂量给药组分别给黄芩总苷元400、200、100 mg/kg；阳性组给予联苯双酯200 mg/kg；阴性组给予2.0‰泊洛沙姆水溶液；模型组和空白组均给予生理盐水。第15天给药6 h后除空白组外均腹腔注射0.1‰四氯化碳油溶液。12 h后称重，取血，分离肝脏，拍照、制匀浆测定丙二醛( MDA)指标；全血离心后取血清测定肝功能指标丙氨酸氨基转移酶( ALT)和天门冬氨酸氨基转移酶( AST)。结果阳性组与高剂量给药组中小鼠肝脏外表光滑红润，无血块和斑点，但光泽差于空白组；中、低剂量给药组红润程度依次下降，并可见瘀斑渐次明显；阴性组和模型组外观苍白，可见成块淤血、瘀斑。模型组、阴性组AST、ALT、MDA指标与空白组相比明显升高；给药组AST、ALT、MDA指标按低、中、高剂量下降，与模型组相比差异有显著性。结论黄芩总黄酮苷元对肝脏有保护作用。
目的：探討黃芩總黃酮苷元( SBTF)對肝髒的保護作用。方法將70隻小鼠隨機均分為7組(空白組、模型組、陰性對照組、聯苯雙酯暘性對照組及高、中、低劑量黃芩總苷元給藥組)，每組10隻，灌胃給藥15天。高、中、低劑量給藥組分彆給黃芩總苷元400、200、100 mg/kg；暘性組給予聯苯雙酯200 mg/kg；陰性組給予2.0‰泊洛沙姆水溶液；模型組和空白組均給予生理鹽水。第15天給藥6 h後除空白組外均腹腔註射0.1‰四氯化碳油溶液。12 h後稱重，取血，分離肝髒，拍照、製勻漿測定丙二醛( MDA)指標；全血離心後取血清測定肝功能指標丙氨痠氨基轉移酶( ALT)和天門鼕氨痠氨基轉移酶( AST)。結果暘性組與高劑量給藥組中小鼠肝髒外錶光滑紅潤，無血塊和斑點，但光澤差于空白組；中、低劑量給藥組紅潤程度依次下降，併可見瘀斑漸次明顯；陰性組和模型組外觀蒼白，可見成塊淤血、瘀斑。模型組、陰性組AST、ALT、MDA指標與空白組相比明顯升高；給藥組AST、ALT、MDA指標按低、中、高劑量下降，與模型組相比差異有顯著性。結論黃芩總黃酮苷元對肝髒有保護作用。
목적：탐토황금총황동감원( SBTF)대간장적보호작용。방법장70지소서수궤균분위7조(공백조、모형조、음성대조조、련분쌍지양성대조조급고、중、저제량황금총감원급약조)，매조10지，관위급약15천。고、중、저제량급약조분별급황금총감원400、200、100 mg/kg；양성조급여련분쌍지200 mg/kg；음성조급여2.0‰박락사모수용액；모형조화공백조균급여생리염수。제15천급약6 h후제공백조외균복강주사0.1‰사록화탄유용액。12 h후칭중，취혈，분리간장，박조、제균장측정병이철( MDA)지표；전혈리심후취혈청측정간공능지표병안산안기전이매( ALT)화천문동안산안기전이매( AST)。결과양성조여고제량급약조중소서간장외표광활홍윤，무혈괴화반점，단광택차우공백조；중、저제량급약조홍윤정도의차하강，병가견어반점차명현；음성조화모형조외관창백，가견성괴어혈、어반。모형조、음성조AST、ALT、MDA지표여공백조상비명현승고；급약조AST、ALT、MDA지표안저、중、고제량하강，여모형조상비차이유현저성。결론황금총황동감원대간장유보호작용。
Objective To study the hepatic protection effect of Scutellaria Baicalensis Total Flavonoid (SBTF). Meth-ods Seventy male Kunming mice were randomly divided into seven groups:blank control group,model group,negative group, bifendate positive group and high-dose,middle-dose,low-dose group. Stomach perfusion was given for 15 days;High-dose,middle-dose,low-dose group were given SBTF 400mg/kg,200mg/kg,100mg/kg each once a day;positive group,negative group,model group and blank control group were given bifendate(200mg/kg),poloxamer solution(2. 0‰),normal saline separately. At the 15th day,6 hours after perfusion,all mice were intraperitoneally injected carbon tetrachloride oil resolution (0. 1‰) except the blank group,and 12h later,mice were weighed and taken for blood;their livers were separated,taking pictures,making homogenate to test MDA. The whole blood was centrifugalled and blood serum was to test ALT and AST and calculate liver index. Result For positive and high-dose group,the surface of mice’s liver was smooth and ruddy with no blood clot or spots,but a little dull com-pared with blank control group;middle-dose and low-dose group had less ruddy sequence with more obvious blood spots;negative and model group were pale,with block of clots and spots. AST,ALT,MDA of negative and model group were apparently higher than blank control group;decreases in the sequent low-dose,middle-dose,high-dose group,but not so evident as positive group.
