现代检验医学杂志
現代檢驗醫學雜誌
현대검험의학잡지
JOURNAL OF MODERN LABORATORY MEDICINE
2014年
6期
78-80
,共3页
孙雪静%万岁桂%刘聪燕%贺景娟%刘艳%张维%赵弘%徐娟
孫雪靜%萬歲桂%劉聰燕%賀景娟%劉豔%張維%趙弘%徐娟
손설정%만세계%류총연%하경연%류염%장유%조홍%서연
流式细胞术%脑脊液%免疫分型%中枢神经系统白血病
流式細胞術%腦脊液%免疫分型%中樞神經繫統白血病
류식세포술%뇌척액%면역분형%중추신경계통백혈병
flow cytometry%cerebrospinal fluid%immunophenotyping%central nervous system leukemia
目的:探讨流式细胞术(flow cytometry,FCM)对患者脑脊液(cerebrospinal fluid,CSF)细胞免疫表型的检测,在诊断中枢神经系统白血病(central nervous system leukemia,CNS-L)中的价值。方法采用多色流式细胞术 CD45/SSC双参数散点图设门方法,对195例患者CSF细胞免疫表型进行分析,同时行细胞形态学检测,比较两种方法的差异。结果195例患者中有71例为非霍奇金淋巴瘤(Non-Hodgkin’s lymphoma,NHL)患者,FCM检测有24例发现异常表型细胞,阳性率为33.80%。经形态学分析,15例发现异常细胞,其阳性率为19.72%,差异具有统计学意义(P=0.022)。58例急性淋巴细胞白血病(acute lymphoma leukemia,ALL)患者,FCM检测有13例发现异常表型细胞,阳性率为22.41%。经形态学分析,12例发现异常细胞,其阳性率为18.97%,差异无统计学意义(P=1.000)。66例急性非淋巴细胞白血病(acute nonlymphocytic leukemia,ANLL)患者,经 FCM和形态学检测均有4例患者检测出异常细胞,其阳性率均为5.97%,差异无统计学意义(P=1.000)。结论脑脊液 FCM检测敏感性高,淋巴瘤患者行 FCM分析更有助于中枢神经白血病的诊断,是细胞形态学检测方法的重要补充。
目的:探討流式細胞術(flow cytometry,FCM)對患者腦脊液(cerebrospinal fluid,CSF)細胞免疫錶型的檢測,在診斷中樞神經繫統白血病(central nervous system leukemia,CNS-L)中的價值。方法採用多色流式細胞術 CD45/SSC雙參數散點圖設門方法,對195例患者CSF細胞免疫錶型進行分析,同時行細胞形態學檢測,比較兩種方法的差異。結果195例患者中有71例為非霍奇金淋巴瘤(Non-Hodgkin’s lymphoma,NHL)患者,FCM檢測有24例髮現異常錶型細胞,暘性率為33.80%。經形態學分析,15例髮現異常細胞,其暘性率為19.72%,差異具有統計學意義(P=0.022)。58例急性淋巴細胞白血病(acute lymphoma leukemia,ALL)患者,FCM檢測有13例髮現異常錶型細胞,暘性率為22.41%。經形態學分析,12例髮現異常細胞,其暘性率為18.97%,差異無統計學意義(P=1.000)。66例急性非淋巴細胞白血病(acute nonlymphocytic leukemia,ANLL)患者,經 FCM和形態學檢測均有4例患者檢測齣異常細胞,其暘性率均為5.97%,差異無統計學意義(P=1.000)。結論腦脊液 FCM檢測敏感性高,淋巴瘤患者行 FCM分析更有助于中樞神經白血病的診斷,是細胞形態學檢測方法的重要補充。
목적:탐토류식세포술(flow cytometry,FCM)대환자뇌척액(cerebrospinal fluid,CSF)세포면역표형적검측,재진단중추신경계통백혈병(central nervous system leukemia,CNS-L)중적개치。방법채용다색류식세포술 CD45/SSC쌍삼수산점도설문방법,대195례환자CSF세포면역표형진행분석,동시행세포형태학검측,비교량충방법적차이。결과195례환자중유71례위비곽기금림파류(Non-Hodgkin’s lymphoma,NHL)환자,FCM검측유24례발현이상표형세포,양성솔위33.80%。경형태학분석,15례발현이상세포,기양성솔위19.72%,차이구유통계학의의(P=0.022)。58례급성림파세포백혈병(acute lymphoma leukemia,ALL)환자,FCM검측유13례발현이상표형세포,양성솔위22.41%。경형태학분석,12례발현이상세포,기양성솔위18.97%,차이무통계학의의(P=1.000)。66례급성비림파세포백혈병(acute nonlymphocytic leukemia,ANLL)환자,경 FCM화형태학검측균유4례환자검측출이상세포,기양성솔균위5.97%,차이무통계학의의(P=1.000)。결론뇌척액 FCM검측민감성고,림파류환자행 FCM분석경유조우중추신경백혈병적진단,시세포형태학검측방법적중요보충。
Objective To explore the clinical diagnostic significance of cerebrospinal fluid (CSF)detection with flow cytome-try (FCM)in central nervous system leukemia (CNS-L).Methods The 1 9 5 cerebrospinal fluid (CSF)samples were ana-lyzed using four-color flow cytometry by CD45/SSC secondary gating strategy and detected by conventional cell morpholo-gy.Results The results showed that 24 (33.80%)patients had abnormal cells by flow cytometry and 15 (19.72%)pa-tients had abnormal cells by conventional cell morphology in 71 Non-Hodgkin’s lymphoma (NHL)patients,respectively. The difference was statistically significant (P=0.022).The results showed that 13 (22.41%)patients had abnormal cells by flow cytometry and 1 2 (1 8.9 7%)patients had abnormal cells by conventional cell morphology in acute lymphoma leuke-mia (ALL)patients,respectively.The difference was no statistically significant (P=1.000).The results showed that 4 (5.9 7%)patients had abnormal cells by flow cytometry and 4 (5.9 7%)patients had abnormal cells by conventional cell morphology in acute nonlymphocytic leukemia (ANLL)patients,respectively.The difference was no statistically significant (P=1.000).Conclusion Cerebrospinal fluid (CSF)detection with flow cytometry (FCM)may be helpful in the diagnosis of central nervous system leukemia in NHL.It has higher sensitivity and is an important supplement to CSF conventional cell morphology.