现代检验医学杂志
現代檢驗醫學雜誌
현대검험의학잡지
JOURNAL OF MODERN LABORATORY MEDICINE
2014年
6期
124-126,130
,共4页
三酰甘油%纯水%反渗透膜%生化
三酰甘油%純水%反滲透膜%生化
삼선감유%순수%반삼투막%생화
triglyceride%purified water%reverse osmosis film%clinical biochemistry
目的：研究实验室纯水系统更换新反渗透膜后，所产纯水的质量是否符合生化检验的要求并评价其影响。方法通过Beckman Coulter生化分析系统配套纯水机天创TCHS-RO／100A自带电阻表和 HM COM-100电导率测量笔连续监测新产纯水中的实时离子含量。用正常纯水在 AU680上重复20次测量 ALT，AST，TBIL，ALP，GGT，TP，ALB，BUN， CR，UA，GLU，TC，TG，HDL，LDL，CK，LDH，P，Ca（对照组），更换为新产纯水后进行相同试验操作（试验组），以评估新产纯水对生化分析的影响。连续5天，每天3次（间隔2 h）监测新产纯水的 TG试剂空白，监测水中甘油水平。结果开机30 min后，HM COM-100测得电导率降至（0.1±0.0）μS／cm，机载电阻表测得新产纯水在开机50 min后稳定在18.25 MΩcm。在用 AU680进行生化分析时，对照组中TG测量值＝1.04 mmol／L，试验组＝21.39 mmol／L，差异有统计学意义（P＜0.01），而除TG其他生化项目偏倚均在5％以内，CV＜1／4CLIA’88，差异无统计学意义（P＞0.05）。TG试剂空白在第5天稳定在A660nm＝0.0156±0.0004。结论新纯水系统在生产约2800 L水后，所产纯水中的甘油可以清除干净。实验室纯水系统在更换反渗透膜后，需要对水质进行连续监测，以评价其对常规生化项目检测的影响。
目的：研究實驗室純水繫統更換新反滲透膜後，所產純水的質量是否符閤生化檢驗的要求併評價其影響。方法通過Beckman Coulter生化分析繫統配套純水機天創TCHS-RO／100A自帶電阻錶和 HM COM-100電導率測量筆連續鑑測新產純水中的實時離子含量。用正常純水在 AU680上重複20次測量 ALT，AST，TBIL，ALP，GGT，TP，ALB，BUN， CR，UA，GLU，TC，TG，HDL，LDL，CK，LDH，P，Ca（對照組），更換為新產純水後進行相同試驗操作（試驗組），以評估新產純水對生化分析的影響。連續5天，每天3次（間隔2 h）鑑測新產純水的 TG試劑空白，鑑測水中甘油水平。結果開機30 min後，HM COM-100測得電導率降至（0.1±0.0）μS／cm，機載電阻錶測得新產純水在開機50 min後穩定在18.25 MΩcm。在用 AU680進行生化分析時，對照組中TG測量值＝1.04 mmol／L，試驗組＝21.39 mmol／L，差異有統計學意義（P＜0.01），而除TG其他生化項目偏倚均在5％以內，CV＜1／4CLIA’88，差異無統計學意義（P＞0.05）。TG試劑空白在第5天穩定在A660nm＝0.0156±0.0004。結論新純水繫統在生產約2800 L水後，所產純水中的甘油可以清除榦淨。實驗室純水繫統在更換反滲透膜後，需要對水質進行連續鑑測，以評價其對常規生化項目檢測的影響。
목적：연구실험실순수계통경환신반삼투막후，소산순수적질량시부부합생화검험적요구병평개기영향。방법통과Beckman Coulter생화분석계통배투순수궤천창TCHS-RO／100A자대전조표화 HM COM-100전도솔측량필련속감측신산순수중적실시리자함량。용정상순수재 AU680상중복20차측량 ALT，AST，TBIL，ALP，GGT，TP，ALB，BUN， CR，UA，GLU，TC，TG，HDL，LDL，CK，LDH，P，Ca（대조조），경환위신산순수후진행상동시험조작（시험조），이평고신산순수대생화분석적영향。련속5천，매천3차（간격2 h）감측신산순수적 TG시제공백，감측수중감유수평。결과개궤30 min후，HM COM-100측득전도솔강지（0.1±0.0）μS／cm，궤재전조표측득신산순수재개궤50 min후은정재18.25 MΩcm。재용 AU680진행생화분석시，대조조중TG측량치＝1.04 mmol／L，시험조＝21.39 mmol／L，차이유통계학의의（P＜0.01），이제TG기타생화항목편의균재5％이내，CV＜1／4CLIA’88，차이무통계학의의（P＞0.05）。TG시제공백재제5천은정재A660nm＝0.0156±0.0004。결론신순수계통재생산약2800 L수후，소산순수중적감유가이청제간정。실험실순수계통재경환반삼투막후，수요대수질진행련속감측，이평개기대상규생화항목검측적영향。
Objective To test if the quality of produced water could come up to the standards of clinical biochemistry,and e-valuate the effect of the produced water after replacement of a new reverse osmosis film of the laboratory water purification system.Methods The ion concentration of the produced water was tested by ohmmeter in Beckman Coulter supporting TianChuang water purification system TCHS-RO/100A and HM COM-100 conductivity meter.The biochemical index ALT,AST,TBIL,ALP,GGT,TP,ALB,BUN,CR,UA,GLU,TC,TG,HDL,LDL,CK,LDH,P,Ca of mixed serum were tested 20 times by AU680 under the condition of normal purified water(control group),and the same operation were repeat-ed after changing to newly produced water(experimental group)to evaluate the effect of newly produced water to biochemi-cal analysis.The triglyceride (TG)reagent blank was tested 3times/d for 5 days to evaluate the glycerin level in the pro-duced water.Results The conductivity of purified water was reduced to (0.1±0.0)μS/cm 30 min after starting up by HM COM-100,and the electric resistance of purified water remained stable at 18.25MΩcm 50min after starting up.During the bi-ochemical analysis by AU680,the value of TG in control group=1.04 mmol/L,in experimental group=21.39 mmol/L,the differences showed statistical significance (P<0.01),but the bias of the others were limited in 5%,CV<1/4CLIA’88,and differences showed no statistical significance (P>0.05).The reagent blank was stable at A660nm=0.015 6±0.000 4 in the fifth day.Conclusion After produced approximately 2 800 L purified water in the system,glycerine could be cleaned up in produced water.It is needed to continuously test the quality of produced water to evaluate the effect of which to clinical bio-chemistry after replacement of a new reverse osmosis film.
