现代检验医学杂志
現代檢驗醫學雜誌
현대검험의학잡지
JOURNAL OF MODERN LABORATORY MEDICINE
2014年
6期
21-24
,共4页
白细胞介素-21%T淋巴细胞 17%调节性T淋巴细胞%原发性胆汁性肝硬化
白細胞介素-21%T淋巴細胞 17%調節性T淋巴細胞%原髮性膽汁性肝硬化
백세포개소-21%T림파세포 17%조절성T림파세포%원발성담즙성간경화
interleukin-2 1%T lymphocytes 1 7%regulatory T lymphocytes%primary biliary cirrhosis
目的：探讨白细胞介素-21（IL-21）在原发性胆汁性肝硬化（PBC）发病机制中的作用。方法2011年1月～2014年1月选取深圳市宝安区福永人民医院和深圳市第三人民医院首诊的38例PBC患者为研究对象，选取35例健康体检者为对照组，酶联免疫吸附法（ELISA）测定两组血清 IL-21，IL-17水平，流式细胞仪测定两组外周血辅助性 T淋巴细胞17（Th17）、调节性T淋巴细胞（Treg）的比率。另通过建立体外试验分析丙酮酸脱氨酶复合体 E2亚基（PDC-E2）多肽与Th17及Treg 细胞水平的关系。结果 PBC 组 IL-17，IL-21，Th17，Th17／Treg 和 Treg 分别为（18.23±4.12）ng／L，（512.25±18.25）ng／L，（3.98±0.98）％，（39.25±3.25）％和（3.11±0.85）％，对照组分别为（7.89±3.26）ng／L，（212.98±21.52）ng／L，（0.92±0.21）％，（12.98±3.12）％和（8.36±1.12）％，差异有统计学意义（t＝8.886，7.021，4.448，10.112，5.125；P值均＜0.05）。体外试验表明，加入 IL-21的培养体系中，Th17（2.41±0.82）％，Th17／Treg 细胞比例（32.98±3.12）％显著高于空白对照组（0.98±0.21）％，（12.25±3.25）％，而 Treg（4.75±0.68）％则低于空白对照组（11.85±0.96）％，差异有统计学意义（t＝8.521，6.489，8.236；P＜0.05）。结论 IL-21水平升高与 PBC发病具有密切的关系，其可通过促进PDC-E2反应而引起Th17／Treg免疫失衡，从而介导PBC的发生。
目的：探討白細胞介素-21（IL-21）在原髮性膽汁性肝硬化（PBC）髮病機製中的作用。方法2011年1月～2014年1月選取深圳市寶安區福永人民醫院和深圳市第三人民醫院首診的38例PBC患者為研究對象，選取35例健康體檢者為對照組，酶聯免疫吸附法（ELISA）測定兩組血清 IL-21，IL-17水平，流式細胞儀測定兩組外週血輔助性 T淋巴細胞17（Th17）、調節性T淋巴細胞（Treg）的比率。另通過建立體外試驗分析丙酮痠脫氨酶複閤體 E2亞基（PDC-E2）多肽與Th17及Treg 細胞水平的關繫。結果 PBC 組 IL-17，IL-21，Th17，Th17／Treg 和 Treg 分彆為（18.23±4.12）ng／L，（512.25±18.25）ng／L，（3.98±0.98）％，（39.25±3.25）％和（3.11±0.85）％，對照組分彆為（7.89±3.26）ng／L，（212.98±21.52）ng／L，（0.92±0.21）％，（12.98±3.12）％和（8.36±1.12）％，差異有統計學意義（t＝8.886，7.021，4.448，10.112，5.125；P值均＜0.05）。體外試驗錶明，加入 IL-21的培養體繫中，Th17（2.41±0.82）％，Th17／Treg 細胞比例（32.98±3.12）％顯著高于空白對照組（0.98±0.21）％，（12.25±3.25）％，而 Treg（4.75±0.68）％則低于空白對照組（11.85±0.96）％，差異有統計學意義（t＝8.521，6.489，8.236；P＜0.05）。結論 IL-21水平升高與 PBC髮病具有密切的關繫，其可通過促進PDC-E2反應而引起Th17／Treg免疫失衡，從而介導PBC的髮生。
목적：탐토백세포개소-21（IL-21）재원발성담즙성간경화（PBC）발병궤제중적작용。방법2011년1월～2014년1월선취심수시보안구복영인민의원화심수시제삼인민의원수진적38례PBC환자위연구대상，선취35례건강체검자위대조조，매련면역흡부법（ELISA）측정량조혈청 IL-21，IL-17수평，류식세포의측정량조외주혈보조성 T림파세포17（Th17）、조절성T림파세포（Treg）적비솔。령통과건입체외시험분석병동산탈안매복합체 E2아기（PDC-E2）다태여Th17급Treg 세포수평적관계。결과 PBC 조 IL-17，IL-21，Th17，Th17／Treg 화 Treg 분별위（18.23±4.12）ng／L，（512.25±18.25）ng／L，（3.98±0.98）％，（39.25±3.25）％화（3.11±0.85）％，대조조분별위（7.89±3.26）ng／L，（212.98±21.52）ng／L，（0.92±0.21）％，（12.98±3.12）％화（8.36±1.12）％，차이유통계학의의（t＝8.886，7.021，4.448，10.112，5.125；P치균＜0.05）。체외시험표명，가입 IL-21적배양체계중，Th17（2.41±0.82）％，Th17／Treg 세포비례（32.98±3.12）％현저고우공백대조조（0.98±0.21）％，（12.25±3.25）％，이 Treg（4.75±0.68）％칙저우공백대조조（11.85±0.96）％，차이유통계학의의（t＝8.521，6.489，8.236；P＜0.05）。결론 IL-21수평승고여 PBC발병구유밀절적관계，기가통과촉진PDC-E2반응이인기Th17／Treg면역실형，종이개도PBC적발생。
Objective To investigate the pathogenesis of interleukin-21 (IL-21)role in primary biliary cirrhosis (PBC).Meth-ods 38 cases of PBC were selected for the study.35 caeses of healthy subjects were selected as the control group in January 2012 to January 2014 from Shenzhen Fuyong People’s Hospital of Baoan District and the Third People’s Hospital of Shenz-hen.The levels of IL-21,IL-17 of two groups were measured with Enzyme-linked immunosorbent assay (ELISA).The levels of T helper lymphocytes 17 (Th17),regulatory T cells (Treg)cells ratios of two groups were measured with flow cytome-try.The relationship of IL-2 1 and liver function were analyzed with pearson correlation.Another analysis of pyruvate through the establishment of in vitro deaminase complex E2 subunit (PDC-E2)peptides relationship with Th17 and Treg cell.Results The levles of IL-17 (18.23±4.12)ng/L,IL-21 (512.25±18.25)ng/L,Th17 (3.98±0.98)%,Th17/Treg (39.25±3.25)% of PBC group were significantly higher than the control group (7.89±3.26)ng/L,(212.98±21.52)ng/L,(0.92±0.21)% and (12.98±3.12)%,while the PBC group Treg (3.11±0.85)% were lower than the control group (8.36±1.12)%.IL-21 were positive correlation with IL-17,Th17 (t=8.886,7.021,4.448,10.112,5.125,P<0.05).In vitro experiments showed that the addition of IL-21 in the culture system,the levels of Th17 (2.41±0.82)%,Th17/Treg cells(32.98±3.12)% were significantly higher than the control group(0.98±0.21)%,(12.25±3.25)%,while Treg (4.75 ±0.68)% were lower than the control group(11.85±0.96)%,the difference was statistically significant (8.521,6.489, 8.236,P<0.05).Conclusion IL-21 levels increased with the pathogenesis of PBC has a close relationship,which can pro-mote the reaction of PDC-E2 Th1 7/Treg immune imbalance caused thereby occurs mediated by PBC.
