现代检验医学杂志
現代檢驗醫學雜誌
현대검험의학잡지
JOURNAL OF MODERN LABORATORY MEDICINE
2014年
6期
55-58
,共4页
任晓娟%王敬春%储海丹%丛辉
任曉娟%王敬春%儲海丹%叢輝
임효연%왕경춘%저해단%총휘
增殖诱导配体%RNA干扰%人基质金属蛋白酶抑制因-3%多配体蛋白聚糖-1%基质金属蛋白酶-9
增殖誘導配體%RNA榦擾%人基質金屬蛋白酶抑製因-3%多配體蛋白聚糖-1%基質金屬蛋白酶-9
증식유도배체%RNA간우%인기질금속단백매억제인-3%다배체단백취당-1%기질금속단백매-9
APRIL%RNA interference%TIMP-3%syndecan-1%MMP-9
目的：探讨增殖诱导配体（APRIL）基因在 SW480裸鼠移植瘤中的作用。方法建立人结直肠癌（CRC）裸鼠移植瘤模型并随机分为3组，瘤块内分别注射 APRIL siRNA、空载体和 PBS液。RT-PCR和免疫组织化学法（IHC）检测 A-PRIL水平和蛋白表达，人基质金属蛋白酶抑制因子-3（IHC检测TIMP-3），多配体蛋白聚糖-1（Syndecan-1，又名CD138）和基质金属蛋白酶-9（MMP-9）蛋白表达。结果①注射PBS组裸鼠移植瘤瘤块质量（2.15±0.30 g）明显高于注射 APRIL siRNA组（0.95±0.15 g，P＜0.05），与注射空载体组（2.20±0.25 g）比较差异无统计学意义（P＞0.05）。②注射 PBS组裸鼠 APRIL mRNA／18S rRNA比值（2.48±0.25）明显高于注射 APRIL siRNA组（0.39±0.15，P＜0.05），与注射空载体组（2.51±0.30）比较差异无统计学意义（P＞0.05）。③注射 APRIL siRNA 明显抑制 SW480细胞侵袭转移，3组 TIMP-3 Allred得分为7.70±0.35，1.10±0.16和1.15±0.12；Syndecan-1蛋白分别为7.80±0.30，1.05±0.20和1.10±0.22；MMP-9蛋白分别为1.20±0.10，8.00±0.25和8.20±0.20。结论 APRIL与人结直肠癌细胞 SW480的生长和转移有密切关系。
目的：探討增殖誘導配體（APRIL）基因在 SW480裸鼠移植瘤中的作用。方法建立人結直腸癌（CRC）裸鼠移植瘤模型併隨機分為3組，瘤塊內分彆註射 APRIL siRNA、空載體和 PBS液。RT-PCR和免疫組織化學法（IHC）檢測 A-PRIL水平和蛋白錶達，人基質金屬蛋白酶抑製因子-3（IHC檢測TIMP-3），多配體蛋白聚糖-1（Syndecan-1，又名CD138）和基質金屬蛋白酶-9（MMP-9）蛋白錶達。結果①註射PBS組裸鼠移植瘤瘤塊質量（2.15±0.30 g）明顯高于註射 APRIL siRNA組（0.95±0.15 g，P＜0.05），與註射空載體組（2.20±0.25 g）比較差異無統計學意義（P＞0.05）。②註射 PBS組裸鼠 APRIL mRNA／18S rRNA比值（2.48±0.25）明顯高于註射 APRIL siRNA組（0.39±0.15，P＜0.05），與註射空載體組（2.51±0.30）比較差異無統計學意義（P＞0.05）。③註射 APRIL siRNA 明顯抑製 SW480細胞侵襲轉移，3組 TIMP-3 Allred得分為7.70±0.35，1.10±0.16和1.15±0.12；Syndecan-1蛋白分彆為7.80±0.30，1.05±0.20和1.10±0.22；MMP-9蛋白分彆為1.20±0.10，8.00±0.25和8.20±0.20。結論 APRIL與人結直腸癌細胞 SW480的生長和轉移有密切關繫。
목적：탐토증식유도배체（APRIL）기인재 SW480라서이식류중적작용。방법건립인결직장암（CRC）라서이식류모형병수궤분위3조，류괴내분별주사 APRIL siRNA、공재체화 PBS액。RT-PCR화면역조직화학법（IHC）검측 A-PRIL수평화단백표체，인기질금속단백매억제인자-3（IHC검측TIMP-3），다배체단백취당-1（Syndecan-1，우명CD138）화기질금속단백매-9（MMP-9）단백표체。결과①주사PBS조라서이식류류괴질량（2.15±0.30 g）명현고우주사 APRIL siRNA조（0.95±0.15 g，P＜0.05），여주사공재체조（2.20±0.25 g）비교차이무통계학의의（P＞0.05）。②주사 PBS조라서 APRIL mRNA／18S rRNA비치（2.48±0.25）명현고우주사 APRIL siRNA조（0.39±0.15，P＜0.05），여주사공재체조（2.51±0.30）비교차이무통계학의의（P＞0.05）。③주사 APRIL siRNA 명현억제 SW480세포침습전이，3조 TIMP-3 Allred득분위7.70±0.35，1.10±0.16화1.15±0.12；Syndecan-1단백분별위7.80±0.30，1.05±0.20화1.10±0.22；MMP-9단백분별위1.20±0.10，8.00±0.25화8.20±0.20。결론 APRIL여인결직장암세포 SW480적생장화전이유밀절관계。
Objective To investigate the role of APRIL in SW480 cell line.Methods The CRC model was established in the nude mice,all the mice were divided into 3 groups,the mice were separately treated with APRIL siRNA,pGC-vector and PBS solution.The APRIL mRNA was detected by RT-PCR and the APRIL protein was surveyed by the way of immunohis-tochemistry (IHC).The proteins of TIMP-3,Syndecan-1and MMP-9 also were assessed by IHC.Results ①Tumor mass in the group of nude mice injected with PBS (2.15±0.30 g)was significantly higher than the injection APRIL siRNA group (0.95±0.15 g,P<0.05),and was not statistically significant (P>0.05)compared with the injection of empty vector group (2.20±0.25 g).②APRIL mRNA/18S rRNA ratio (2.48±0.25)in the group of mice injected with PBS was signifi-cantly higher than the injection APRIL siRNA group (0.39±0.15,P<0.05),and was not statistically significant (P>0.05)compared with the injection of empty vector group (2.51±0.30).③SW480 cells injected with APRIL siRNA signifi-cantly inhibited invasion and metastasis.TIMP-3 Allred scores in three groups were 7.70±0.35,1.10±0.16 and 1.15± 0.12,Syndecan-1 protein was 7.80±0.30,1.05±0.20 and 1.10±0.22 MMP-9 protein was 1.20 ±0.10,8.00±0.25 and 8.20±0.20,respectively.Conlusion APRIL was closely connected with the growth and metabasis of CRC.
