茶叶科学技术
茶葉科學技術
다협과학기술
TEA SCIENCE AND TECHNOLOGY
2014年
4期
32-36
,共5页
杨军%孔祥瑞%王让剑%郑国华
楊軍%孔祥瑞%王讓劍%鄭國華
양군%공상서%왕양검%정국화
茶树%自然杂交%EST-SSR%亲缘关系%遗传多样性
茶樹%自然雜交%EST-SSR%親緣關繫%遺傳多樣性
다수%자연잡교%EST-SSR%친연관계%유전다양성
tea plant%natural hybrids%EST-SSR%genetic relationship%genetic diversity
利用 EST-SSR 对茶树品种铁观音、黄棪、金牡丹及其自然杂交后代,进行初步遗传多样性和亲缘关系分析。14对EST-SSR标记共检测到等位位点62个,平均检测到4.43个,Shannon信息指数1.22,Simpson指数0.70。在相似系数0.73时,金牡丹自然杂交后代可分为4组。结果表明,第I组与铁观音亲缘关系最近,第II组与金牡丹亲缘关系最近,第II组组内遗传多样性较高;4个组间在树幅、叶片长、叶片宽等性状上达到显著性差异,每组在性状上都具有一定的群体特征。
利用 EST-SSR 對茶樹品種鐵觀音、黃棪、金牡丹及其自然雜交後代,進行初步遺傳多樣性和親緣關繫分析。14對EST-SSR標記共檢測到等位位點62箇,平均檢測到4.43箇,Shannon信息指數1.22,Simpson指數0.70。在相似繫數0.73時,金牡丹自然雜交後代可分為4組。結果錶明,第I組與鐵觀音親緣關繫最近,第II組與金牡丹親緣關繫最近,第II組組內遺傳多樣性較高;4箇組間在樹幅、葉片長、葉片寬等性狀上達到顯著性差異,每組在性狀上都具有一定的群體特徵。
이용 EST-SSR 대다수품충철관음、황염、금모단급기자연잡교후대,진행초보유전다양성화친연관계분석。14대EST-SSR표기공검측도등위위점62개,평균검측도4.43개,Shannon신식지수1.22,Simpson지수0.70。재상사계수0.73시,금모단자연잡교후대가분위4조。결과표명,제I조여철관음친연관계최근,제II조여금모단친연관계최근,제II조조내유전다양성교고;4개조간재수폭、협편장、협편관등성상상체도현저성차이,매조재성상상도구유일정적군체특정。
Genetic diversity and relationship amongCamellia sinensis“Tie guanyin”,“Huangdan”, and“Jinmudan” and its natural hybrid prodenies, were researched with EST-ESSR markers. Using 14 EST-SSR primers, 62 alleles were generated and its average value was 4.43(ranged between 1 and 7). The Shannon’s information index and Simpson index were 0.485 and 0.70 respectively. According to the similarity coefficient at 0.73, the natural hybrid progenies ofJinmudan could be clustered into 4 groups. From the results, Group I was a close relative ofTie guanyin. Group II was close related toJinmudan and expressed the high genetic variation. There were significant differences in tree width, leaf length and leaf width among 4 group progenies ofJinmudan, furthermore, each group possessed a certain group characteristics.