中国康复理论与实践
中國康複理論與實踐
중국강복이론여실천
CHINESE JOURNAL OF REHABILITATION THEORY & PRACTICE
2015年
2期
148-152
,共5页
梁羽%李灿%罗兰%杨拯%王强%马俊%王洋洋%张璐璐%杨维%易芳
樑羽%李燦%囉蘭%楊拯%王彊%馬俊%王洋洋%張璐璐%楊維%易芳
량우%리찬%라란%양증%왕강%마준%왕양양%장로로%양유%역방
脊髓损伤%肠道%功能障碍%小鼠
脊髓損傷%腸道%功能障礙%小鼠
척수손상%장도%공능장애%소서
spinal cord injury%intestine%dysfunction%mice
目的：探讨脊髓损伤后短期内小鼠肠道功能的变化。方法将105只昆明种小鼠随机分为正常组(A组，n=30)、假手术组(B组，n=30)和模型组(C组，n=45)。A组不作处理，B组仅暴露脊髓，不夹持。C组采用动脉瘤夹夹持T10处脊髓，复制脊髓损伤模型。分别于术后12 h、24 h、48 h测定小鼠回肠肌电慢波及平滑肌收缩力，并做回肠HE染色。结果脊髓损伤后12 h、24 h、48 h，C组小鼠肌电频率和振幅低于A组和B组(P<0.05)，收缩力振幅低于A组和B组(P<0.05)，但24 h、48 h后收缩力频率高于A组和B组(P<0.05)。C组各时间点肠黏膜评分均较A组和B组高(P<0.05)。结论小鼠脊髓损伤后早期，肠道平滑肌肌电活动减弱，收缩力减小，肠黏膜轻度损伤。
目的：探討脊髓損傷後短期內小鼠腸道功能的變化。方法將105隻昆明種小鼠隨機分為正常組(A組，n=30)、假手術組(B組，n=30)和模型組(C組，n=45)。A組不作處理，B組僅暴露脊髓，不夾持。C組採用動脈瘤夾夾持T10處脊髓，複製脊髓損傷模型。分彆于術後12 h、24 h、48 h測定小鼠迴腸肌電慢波及平滑肌收縮力，併做迴腸HE染色。結果脊髓損傷後12 h、24 h、48 h，C組小鼠肌電頻率和振幅低于A組和B組(P<0.05)，收縮力振幅低于A組和B組(P<0.05)，但24 h、48 h後收縮力頻率高于A組和B組(P<0.05)。C組各時間點腸黏膜評分均較A組和B組高(P<0.05)。結論小鼠脊髓損傷後早期，腸道平滑肌肌電活動減弱，收縮力減小，腸黏膜輕度損傷。
목적：탐토척수손상후단기내소서장도공능적변화。방법장105지곤명충소서수궤분위정상조(A조，n=30)、가수술조(B조，n=30)화모형조(C조，n=45)。A조불작처리，B조부폭로척수，불협지。C조채용동맥류협협지T10처척수，복제척수손상모형。분별우술후12 h、24 h、48 h측정소서회장기전만파급평활기수축력，병주회장HE염색。결과척수손상후12 h、24 h、48 h，C조소서기전빈솔화진폭저우A조화B조(P<0.05)，수축력진폭저우A조화B조(P<0.05)，단24 h、48 h후수축력빈솔고우A조화B조(P<0.05)。C조각시간점장점막평분균교A조화B조고(P<0.05)。결론소서척수손상후조기，장도평활기기전활동감약，수축력감소，장점막경도손상。
Objective To investigate the influence of spinal cord injury on intestinal function in mice after a short period. Methods 105 Kunming mice were randomly assigned to normal group (group A, n=30), sham group (group B, n=30) and model group (group C, n=45). Group A received no treatment, group B was only exposed the spinal cord, while group C was induced by a constant compression with a modified arterial forcep at T10 to establish spinal cord injury model. 12 hours, 24 hours and 48 hours after modeling, myoelectric slow wave activity and tension activity of the ileum were tested, and HE staining was used. Results Compared with groups A and B, the slow wave ac-tivity was significantly weaker in group C at every time point (P<0.05), so was the amplitude of tension activity (P<0.05). Frequency of ten-sion activity was obviously higher in group C than in groups A and B 24 hours and 48 hours after modeling (P<0.05). The injury scores were higher in group C than in groups A and B (P<0.05). Conclusion There is reduce in myoelectric slow wave activity, tension activity of the ile-um and mild injury in intestinal mucosa in mice after spinal cord injury in the early stage.
