CAJ | 학술논문

目的：建立了同时检测鸡蛋中8种四环素类药物残留的HPLC-DAD方法，建立了它们的相对保留时间和多波长检测的峰面积比值数据库，通过数据库建立了一个数据检索平台，并将其用于鸡蛋中的四环素类药物的残留的快速筛查。方法先建立起一个同时检测8种药物的HPLC-DAD色谱分析方法，然后根据所得的HPLC-DAD色谱图中对照品的信息，建立起一个包含各对照品的相对保留时间比值和多波长检测峰面积比值的数据库，并以此为基础建立起一个检索平台；实际检测时，如果样品中出现可疑物质，则可将疑似峰的保留时间和多波长检测的峰面积输入库中进行检索，达到快速筛查的目的。结果8种四环素类药物在建立的色谱条件下实现了完全分离；数字对照品检索数据库在实际检测中，显示出简单方便，准确可靠的优势。结论该方法建立的数字对照品库可以代替实际对照品，在监督现场对鸡蛋中是否含有四环素类药物残留进行快速筛查和检定。
목적：건립료동시검측계단중8충사배소류약물잔류적HPLC-DAD방법，건립료타문적상대보류시간화다파장검측적봉면적비치수거고，통과수거고건립료일개수거검색평태，병장기용우계단중적사배소류약물적잔류적쾌속사사。방법선건립기일개동시검측8충약물적HPLC-DAD색보분석방법，연후근거소득적HPLC-DAD색보도중대조품적신식，건립기일개포함각대조품적상대보류시간비치화다파장검측봉면적비치적수거고，병이차위기출건립기일개검색평태；실제검측시，여과양품중출현가의물질，칙가장의사봉적보류시간화다파장검측적봉면적수입고중진행검색，체도쾌속사사적목적。결과8충사배소류약물재건립적색보조건하실현료완전분리；수자대조품검색수거고재실제검측중，현시출간단방편，준학가고적우세。결론해방법건립적수자대조품고가이대체실제대조품，재감독현장대계단중시부함유사배소류약물잔류진행쾌속사사화검정。
Objective To establish a rapid determination of eight tetracycline residues in eggs by HPLC-DAD, including 4-epianhydro-tetracycline oxytraoycline, epitetracycline hydrochloride, methacycline, tetracycline, doxycycline, chlortetracycline and minocycline. Based on the HPLC-DAD spectrums, a numerical reference substance database including the adjusted relative retention times and the peak area rations was developed.A data search platform was integrated with the database and used for rapid screening of eight tetracy-cline residues in eggs.Methods Firstly, HPLC-DAD spectrums were collected.Then a numerical reference substance database in-cluding the adjusted relative retention times and the peak area rations was established.Based on this database, a digital reference sub-stance search platform was set up.For a suspicious substance in testing, we can compare its relative retention time and the peak area rations with the information in the database to identify this substance.Results 8 tetracyclines were well separated based on the chrom-atographic conditions.This method has been successfully applied to real sample analysis.Conclusion The reference substance data-base can be used to rapidly screen the eggs to judge whether the tetracycline residues are in eggs when surveillance on-site .