CAJ | 학술논문

目的：观察添加人AB型血清或胎牛血清（FBS）培养基培养的人外周血单个核细胞（PBMC）在结核杆菌（M．tb）全菌裂解物（ WCL）的刺激下增殖差异。方法健康成人PBMC 1×109· L－1，分别加入WCL 20 mg· L－1，或者同时给予人重组白细胞介素－2（rhIL-2）50 U· mL－1，植物血凝素（PHA）5 mg· L－1，同时设置空白对照组。分别在含10％人AB型血清的RP-MI1640培养液和10％FBS的RPMIl640培养液中培养6 d后，用倒置显微镜观察培养结果，运用流式细胞术检测淋巴细胞增殖情况。结果添加人AB型血清培养基培养条件下，M．tb WCL对淋巴细胞增殖效果较同样条件下添加FBS培养的增殖效果明显，差异有统计学意义（ P＜0．05）。在WCL和rhIL-2混合组中同样是添加人AB型血清培养增殖效果明显，差异有统计学意义（ P＜0．05）。结论当以M．tb WCL为抗原的刺激人PBMC时，淋巴细胞在添加人AB型血清培养液中比在添加FBS的培养液中增殖效果更加明显。
목적：관찰첨가인AB형혈청혹태우혈청（FBS）배양기배양적인외주혈단개핵세포（PBMC）재결핵간균（M．tb）전균렬해물（ WCL）적자격하증식차이。방법건강성인PBMC 1×109· L－1，분별가입WCL 20 mg· L－1，혹자동시급여인중조백세포개소－2（rhIL-2）50 U· mL－1，식물혈응소（PHA）5 mg· L－1，동시설치공백대조조。분별재함10％인AB형혈청적RP-MI1640배양액화10％FBS적RPMIl640배양액중배양6 d후，용도치현미경관찰배양결과，운용류식세포술검측림파세포증식정황。결과첨가인AB형혈청배양기배양조건하，M．tb WCL대림파세포증식효과교동양조건하첨가FBS배양적증식효과명현，차이유통계학의의（ P＜0．05）。재WCL화rhIL-2혼합조중동양시첨가인AB형혈청배양증식효과명현，차이유통계학의의（ P＜0．05）。결론당이M．tb WCL위항원적자격인PBMC시，림파세포재첨가인AB형혈청배양액중비재첨가FBS적배양액중증식효과경가명현。
Objective To observe the proliferation difference of human peripheral blood mononucelear cells ( PBMCs) which were stim-ulated with Mycobacterium tuberculosis ( M.tb) Whole Cell Lysate ( WCL) in human AB serum cell culture medium and fatal bovine serum ( FBS) cell culture medium.Methods PBMCs from healthy adults were stimulated by WCL or WCL with recombinant human interleukin 2 ( rhIL-2) or phytohemagglutinin ( PHA) or none as control in four groups.Each of the groups was cultured with RPMI-1640 medium containing 10%AB serum or 10%FBS for six days respectively.The proliferation of lymphocytes was carefully investiga-ted under the inverted microscope and flow cytometry.Results The proliferation of lymphocytes stimulated by WCL in medium with 10%AB serum is better than that in medium with 10%FBS (P<0.05).Similar results were obtained when PBMCs were stimulated by WCL and rhIL-2 (P<0.05) .Conclusion When human PBMCs were stimulated with M.tb WCL,the proliferation of lymphocytes was better in the culture medium supplemented with human AB serum than in that with FBS serum.