CAJ | 학술논문

目的：观察电针内关穴预处理对心肌缺血再灌注损伤兔CK及心肌细胞活性的影响,为证实心包经穴位与心之间的相对特异性联系提供实验依据。方法将40只新西兰大耳白兔随机分为A组、B组、C组、D组和E组,每组8只。A组为假手术组；B组为缺血再灌注模型组,于左冠状动脉前降支阻断冠脉30 min,再灌注60 min；C组、D组和E组在造模前7 d,每日分别取内关、列缺及合谷给予电针刺激20 min。比较各组肌酸激酶(CK)及心肌 formazan 含量变化。结果 B 组、C 组、D组和E组血清CK及心肌formazan含量与A组比较,差异均具有统计学意义(P＜0.01,P＜0.05)。C组、D组和E组血清CK含量与B组比较,差异均具有统计学意义(P＜0.01)。C组心肌formazan含量与B组比较,差异具有统计学意义(P＜0.05)。D组和E组血清CK 及心肌formazan含量与C组比较,差异均具有统计学意义(P＜0.01,P＜0.05)。结论电针内关穴能减轻心肌缺血再灌注对心肌细胞的损伤,从而对心脏产生保护作用。
목적：관찰전침내관혈예처리대심기결혈재관주손상토CK급심기세포활성적영향,위증실심포경혈위여심지간적상대특이성련계제공실험의거。방법장40지신서란대이백토수궤분위A조、B조、C조、D조화E조,매조8지。A조위가수술조；B조위결혈재관주모형조,우좌관상동맥전강지조단관맥30 min,재관주60 min；C조、D조화E조재조모전7 d,매일분별취내관、렬결급합곡급여전침자격20 min。비교각조기산격매(CK)급심기 formazan 함량변화。결과 B 조、C 조、D조화E조혈청CK급심기formazan함량여A조비교,차이균구유통계학의의(P＜0.01,P＜0.05)。C조、D조화E조혈청CK함량여B조비교,차이균구유통계학의의(P＜0.01)。C조심기formazan함량여B조비교,차이구유통계학의의(P＜0.05)。D조화E조혈청CK 급심기formazan함량여C조비교,차이균구유통계학의의(P＜0.01,P＜0.05)。결론전침내관혈능감경심기결혈재관주대심기세포적손상,종이대심장산생보호작용。
Objective To investigate the effect of pretreatment with electroacupuncture at point Neiguan(PC6) on CK and myocardial cell activity in rabbit myocardial ischemia-reperfusion injury and provide an experimental basis for a relatively specific relationship between pericardium meridian points and the heart. Methods Forty New Zealand big ear rabbits were randomly allocated to groups A, B, C, D and E, eight rabbits each. Group A was a sham operation group. Group B was an ischemia-reperfusion model group, in which the left anterior descending coronary artery was occlude for 30 min and reperfused for 60 min. In groups C, D and E, points Neiguan(PC6), Lieque(LU7) and Hegu(LI4) were separately given electroacupuncture stimulation, 20 min every day, at seven days before model making. Creatine kinase (CK) and myocardial formazan contents were compared between the groups. Results There were statistically significant differences in serum CK and myocardial formazan contents between group B, C, D or E and group A (P<0.01, P<0.05). There was a statistically significant differences in serum CK content between group C, D or E and group B (P<0.01). There was a statistically significant differences in myocardial formazan content between groups C and B (P<0.05). There were statistically significant differences in serum CK and myocardial formazan contents between group D or E and group C (P<0.01, P<0.05). Conclusion Electroacupuncture at point Neiguan(PC6) can reduce myocardial ischemia-reperfusion induced injury to myocardial cells to produce a protective effect on the heart.