CAJ | 학술논문

目的：探讨“双固一通”电针法对老年阳虚证模型大鼠肝线粒体形态结构及 ATP 酶活性的影响。方法选用5月龄SD雄性大鼠40只,随机分为A组、B组、C组和D组,每组10只。A组为正常对照组,其余3组大鼠均皮下注射D-半乳糖40 d后再肌肉注射氢化可的松7 d制作老年阳虚证大鼠模型。C组采用“双固一通”电针法,电针关元、足三里、百会穴；D组采用电针中极、阴陵泉、印堂穴治疗。每星期治疗6次,共治疗4星期。治疗后以游泳力竭时间判定各组大鼠抗疲劳能力,并采用透射电镜观察肝线粒体结构、比色法检测各组大鼠肝组织Na+-K+-ATP酶、Ca2+-Mg2+-ATP酶活性。结果与A组比较,B组和D组大鼠平均游泳力竭时间明显缩短(均P＜0.01),肝线粒体内外膜模糊不清,嵴变形、断裂或消失,肝线粒体出现肿胀等改变,Na+-K+-ATP酶、Ca2+-Mg2+-ATP酶活性均下降(P＜0.01,P＜0.05)。C组比B组游泳力竭时间明显延长(P＜0.01),肝线粒体形态结构得到明显改善,Na+-K+-ATP酶、Ca2+-Mg2+-ATP酶活性均明显提高(P＜0.01,P＜0.05)；D组与B组比较,大鼠平均游泳力竭时间缩短(P＜0.05),肝粗面内质网有脱颗粒,肝Na+-K+-ATP酶、Ca2+-Mg2+-ATP酶活性降低(P＜0.05)。结论“双固一通”电针法可改善老年阳虚证模型大鼠阳虚抗疲劳能力,改善肝线粒体超微结构,提高肝Na+-K+-ATP酶、Ca2+-Mg2+-ATP酶活性,促进肝脏能量代谢。
목적：탐토“쌍고일통”전침법대노년양허증모형대서간선립체형태결구급 ATP 매활성적영향。방법선용5월령SD웅성대서40지,수궤분위A조、B조、C조화D조,매조10지。A조위정상대조조,기여3조대서균피하주사D-반유당40 d후재기육주사경화가적송7 d제작노년양허증대서모형。C조채용“쌍고일통”전침법,전침관원、족삼리、백회혈；D조채용전침중겁、음릉천、인당혈치료。매성기치료6차,공치료4성기。치료후이유영력갈시간판정각조대서항피로능력,병채용투사전경관찰간선립체결구、비색법검측각조대서간조직Na+-K+-ATP매、Ca2+-Mg2+-ATP매활성。결과여A조비교,B조화D조대서평균유영력갈시간명현축단(균P＜0.01),간선립체내외막모호불청,척변형、단렬혹소실,간선립체출현종창등개변,Na+-K+-ATP매、Ca2+-Mg2+-ATP매활성균하강(P＜0.01,P＜0.05)。C조비B조유영력갈시간명현연장(P＜0.01),간선립체형태결구득도명현개선,Na+-K+-ATP매、Ca2+-Mg2+-ATP매활성균명현제고(P＜0.01,P＜0.05)；D조여B조비교,대서평균유영력갈시간축단(P＜0.05),간조면내질망유탈과립,간Na+-K+-ATP매、Ca2+-Mg2+-ATP매활성강저(P＜0.05)。결론“쌍고일통”전침법가개선노년양허증모형대서양허항피로능력,개선간선립체초미결구,제고간Na+-K+-ATP매、Ca2+-Mg2+-ATP매활성,촉진간장능량대사。
Objective To explore the effect of “double reinforcing and one unblocking” electroacupuncture on hepatic mitochondrial structure and ATPase activity in a rat model of senile yang deficiency. Methods Forty 5-month-old male SD rats were selected and randomly allocated to groups A, B, C and D, 10 rats each. Group A was a normal control group. A model of senile yang deficiency was made by 40 days’ subdermal injection of D-galactose and then 7 days’ intramuscular injection of hydrocortisone in the other three groups of rats. Group C received treatment with “double reinforcing and one unblocking”electroacupuncture at points Guangyuan(CV4), Zusanli(ST36) and Baihui(GV20); group D, treatment with electroacupuncture at points Zhongji(CV3), Yinlingquan(SP9) and Yintang(GV29). Treatment was given 6 times a week for a total of 4 weeks. After treatment, the anti-fatigue ability was determined by swimming exhaustion time, hepatic mitochondrial structure was observed under a transmission electron microscope and hepatic Na+-K+-ATPase and Ca2+-Mg2+-ATPase activities were measured by colorimetry in every group of rats. Results The mean swimming exhaustion time shortened significantly (all P<0.01); hepatic mitochondrial inner and outer membranes were blurred, mitochondrial ridges deformed, ruptured or disappeared and mitochondria swelled; both Na+-K+-ATPase and Ca2+-Mg2+-ATPase activities decreased (P<0.01, P<0.05) in groups B, C and D of rats compared with group A. Swimming exhaustion time lengthened significantly (P<0.01), hepatic mitochondrial structure improved significantly and both Na+-K+-ATPase and Ca2+-Mg2+-ATPase activities increased significantly (P<0.01, P<0.05) in group C compared with group B. Rat mean swimming exhaustion time shortened (P<0.05), hepatic rough endoplasmic reticula degranulated and hepatic Na+-K+-ATPase and Ca2+-Mg2+-ATPase activities decreased (P<0.05) in group D compared with group B. Conclusion “Double reinforcing and one unblocking”electroacupuncture can improve anti-fatigue ability, ameliorate hepatic mitochondrial ultrastructure, increase hepatic Na+-K+-ATPase and Ca2+-Mg2+-ATPase activities and promote hepatic energy metabolism in a rat model of senile yang deficiency.