CAJ | 학술논문

通过PCR技术，从自行分离的鸭甲肝病毒JS株基因组中扩增出病毒衣壳蛋白VP1完整基因片段，并对该片段进行序列测定及分析。结果显示JS－VP 1与DHAV－A的VP 1基因序列酸核苷酸同源性在62．0％～64．4％之间，与 DHAV－B 的 VP1基因序列酸核苷酸同源性在63．6％～63．8％之间，与DHAV－C的VP1基因序列酸核苷酸同源性在92．3％～98．5％之间，分析表明JS株为鸭甲肝病毒基因C型，血清型分型为韩国新型。进化树表明JS株与SD02株的亲缘关系较进，进一步说明VP 1基因的核苷酸序列是DHAV基因组中的高度可变区，可作为DHAV基因型研究的标记，分离毒株为DHAV的防治及疫苗的设计奠定了基础。
통과PCR기술，종자행분리적압갑간병독JS주기인조중확증출병독의각단백VP1완정기인편단，병대해편단진행서렬측정급분석。결과현시JS－VP 1여DHAV－A적VP 1기인서렬산핵감산동원성재62．0％～64．4％지간，여 DHAV－B 적 VP1기인서렬산핵감산동원성재63．6％～63．8％지간，여DHAV－C적VP1기인서렬산핵감산동원성재92．3％～98．5％지간，분석표명JS주위압갑간병독기인C형，혈청형분형위한국신형。진화수표명JS주여SD02주적친연관계교진，진일보설명VP 1기인적핵감산서렬시DHAV기인조중적고도가변구，가작위DHAV기인형연구적표기，분리독주위DHAV적방치급역묘적설계전정료기출。
VP1 gene of duck hepatitis A virus was amplified from the gene by PCR, and then sequenced. The result of sequence analysis showed that the gene of VP1 of JS strain and DHAV-A shared identities of 62.0%~64.4%, DHAV-B shared identities of 63.6%~63.8%, and DHAV-C shared identities of 92.3%~98.5% which showed that JS strain was DHAV-C and a new serotype strain in Korea. Phylogenetic analysis showed that it had the closest relationship with SD02 strain. This conclusion further illustrated that the nucleotide sequence of VP1 gene was highly variable area of DHAV, and could be used as DHAV genotype research. The isolate will lay a theoretical basis for the disease prevention and vaccine design.