CAJ | 학술논문

目的：探讨十两茶提取物对氧化型低密度脂蛋白（ox-LDL）诱导衰老细胞模型的影响及其可能的机制。方法将人脐静脉内皮细胞（HUVECs）株于含有10%牛血清的DMEM培养液中培养，使用ox-LDL孵育建立衰老细胞模型，用β?半乳糖苷酶染色法评价内皮细胞的衰老状态，分别予以不同剂量十两茶提取物及辛伐他汀预处理，检测培养液中活性氧（ROS）、非对称二甲基精氨酸（ADMA）、二甲基精氨酸二甲胺水解酶（DDAH）以及端粒酶活性水平。结果 ox-LDL诱导的内皮细胞衰老模型中β?半乳糖苷酶染色阳性的细胞数量显著增加，细胞内ROS水平、ADMA水平增强，DDAH和端粒酶活性降低，而十两茶提取物预处理可明显抑制这一过程（P＜0.05）。结论十两茶提取物在ox-LDL诱导的内皮细胞衰老进程中起抑制作用，其机制可能与降低细胞内ROS、ADMA水平以及提高DDAH水平和端粒酶活性有关。
목적：탐토십량다제취물대양화형저밀도지단백（ox-LDL）유도쇠로세포모형적영향급기가능적궤제。방법장인제정맥내피세포（HUVECs）주우함유10%우혈청적DMEM배양액중배양，사용ox-LDL부육건립쇠로세포모형，용β?반유당감매염색법평개내피세포적쇠로상태，분별여이불동제량십량다제취물급신벌타정예처리，검측배양액중활성양（ROS）、비대칭이갑기정안산（ADMA）、이갑기정안산이갑알수해매（DDAH）이급단립매활성수평。결과 ox-LDL유도적내피세포쇠로모형중β?반유당감매염색양성적세포수량현저증가，세포내ROS수평、ADMA수평증강，DDAH화단립매활성강저，이십량다제취물예처리가명현억제저일과정（P＜0.05）。결론십량다제취물재ox-LDL유도적내피세포쇠로진정중기억제작용，기궤제가능여강저세포내ROS、ADMA수평이급제고DDAH수평화단립매활성유관。
Objective To determine the effect of the extract of Shiliang tea (a traditional tea from Anhua county, Hunan Province, China) on the senescence cells induced by oxidized low density lipoprotein (ox-LDL) and investigate the underlying potential mechanism. Methods Human umbilical vein endothelial cells (HUVECs) were cultured in the DMEM containing 10%bovine serum, and then treated with ox-LDL to establish the model of senescent cells.β-galactose glucoside enzyme staining was used to evaluate the senescence of the endothelial cells. Simvastatin (10μmol/L, positive control) and different doses of the extract of Shiliang tea (1.0,2.5 and 5.0g/L) were used to treat the cells before ox-LDL inducement. The levels of reactive oxygen species (ROS) and asymmetric dimethylarginine (ADMA), and the activities of dimethylarginine dimethylaminohydrolase (DDAH) and telomerase in culture medium were detected. Results In the senescence model of the endothelial cells induced by ox-LDL, the amount of positive cells ofβ-galactose glucoside enzyme staining was significantly increased, the levels of ROS and ADMA were enhanced, and the activities of DDAH and telomerase were reduced. However, the pretreatment of the extract of Shiliang tea restrained the process apparently (P<0.05). Conclusion The extract of Shiliang tea plays a restraining role in the senescence process of endothelial cells induced by ox-LDL, which might be through reducing the levels of ROS and ADMA and improving the activities of DDAH and telomerase.