中华口腔医学杂志
中華口腔醫學雜誌
중화구강의학잡지
Chinese Journal of Stomatology
2015年
1期
33-37
,共5页
成纤维细胞%钛%微沟槽%接触诱导
成纖維細胞%鈦%微溝槽%接觸誘導
성섬유세포%태%미구조%접촉유도
Fibroblasts%Titanium%Microgroove%Contact guidance
目的 比较钛表面不同尺寸微沟槽对人牙龈成纤维细胞(human gingival fibroblast,HGF)接触诱导效应的差异,以期为种植体穿龈部分微沟槽尺寸设计提供依据.方法设计微沟槽宽度与间距相等,利用光刻技术在硅片表面制作宽度分别为15、30、60 μm,深度分别为5和10 μm的微沟槽,分别命名为T15/5、T15/10、T30/5 、T30/10、T60/5、T60/10组,各组硅片表面溅射钛;以溅射钛的硅片为对照组(每组样本量为7).免疫荧光观察细胞沿沟槽排列的形态,通过细胞核排列一致性比例及细胞核形变率进行定量比较.结果 细胞核排列一致性比例及细胞核形变率各微沟槽组均大于对照组,细胞核排列一致性比例T60/10组(0.937±0.024)最高,T15/5组(0.660±0.016)最低.细胞核形变率T60/10组(3.555±0.205)最高,T15/5组(1.819±0.011)最低.结论 不同尺寸微沟槽均可对HGF起接触诱导作用,程度随沟槽宽度和深度的增加而增大.
目的 比較鈦錶麵不同呎吋微溝槽對人牙齦成纖維細胞(human gingival fibroblast,HGF)接觸誘導效應的差異,以期為種植體穿齦部分微溝槽呎吋設計提供依據.方法設計微溝槽寬度與間距相等,利用光刻技術在硅片錶麵製作寬度分彆為15、30、60 μm,深度分彆為5和10 μm的微溝槽,分彆命名為T15/5、T15/10、T30/5 、T30/10、T60/5、T60/10組,各組硅片錶麵濺射鈦;以濺射鈦的硅片為對照組(每組樣本量為7).免疫熒光觀察細胞沿溝槽排列的形態,通過細胞覈排列一緻性比例及細胞覈形變率進行定量比較.結果 細胞覈排列一緻性比例及細胞覈形變率各微溝槽組均大于對照組,細胞覈排列一緻性比例T60/10組(0.937±0.024)最高,T15/5組(0.660±0.016)最低.細胞覈形變率T60/10組(3.555±0.205)最高,T15/5組(1.819±0.011)最低.結論 不同呎吋微溝槽均可對HGF起接觸誘導作用,程度隨溝槽寬度和深度的增加而增大.
목적 비교태표면불동척촌미구조대인아간성섬유세포(human gingival fibroblast,HGF)접촉유도효응적차이,이기위충식체천간부분미구조척촌설계제공의거.방법설계미구조관도여간거상등,이용광각기술재규편표면제작관도분별위15、30、60 μm,심도분별위5화10 μm적미구조,분별명명위T15/5、T15/10、T30/5 、T30/10、T60/5、T60/10조,각조규편표면천사태;이천사태적규편위대조조(매조양본량위7).면역형광관찰세포연구조배렬적형태,통과세포핵배렬일치성비례급세포핵형변솔진행정량비교.결과 세포핵배렬일치성비례급세포핵형변솔각미구조조균대우대조조,세포핵배렬일치성비례T60/10조(0.937±0.024)최고,T15/5조(0.660±0.016)최저.세포핵형변솔T60/10조(3.555±0.205)최고,T15/5조(1.819±0.011)최저.결론 불동척촌미구조균가대HGF기접촉유도작용,정도수구조관도화심도적증가이증대.
Objective To compared the difference incontact guidance activity among microgroove surfaces with different sizes of human gingival fibroblast(HGF),with the hope of providing basis for size selection of microgroove for transmucosal part of dental implant.Methods Basing on the size of HGF,microgroove titanium surfaces were fabricated by photolithography with parallel grooves:15,30 or 60 μm in width and 5 or 10 μm in depth.The groups that used different microgroove surfaces were denoted as T15/5,T15/10,T30/5,T30/10,T60/5,and T60/10.Group T0(the control meanwhile was a sputter of titanium on a simple planar silicon substrate).The morphology that HGF arranged along the groove was analyzed by immunofluorescence staining.Difference in contact guidance activity was quantitatively compared basing on the consistency of nucleus arrangement and deformation ratio.Results Microgroove groups had significantly higher consistency of nucleus arrangement and deformation ratio compared to the control group,with T60/10 had the highest consistency of 0.937±0.024,and T15/5 had the lowest consistency of 0.660± 0.016 and T60/10 had the highest deformation ratio of 3.555±0.205,and T15/5 had the lowest deformation ratio of 1.819±0.011.Conclusions Microgroove surfaces of all the different sizes show contact guidance activity on HGF,and the contact guidance activity increases with the increase of width and depth.
