国际医药卫生导报
國際醫藥衛生導報
국제의약위생도보
INTERNATIONAL MEDICINE & HEALTH GUIDANCE NEWS
2015年
1期
43-45
,共3页
糖尿病视网膜病变%血管内皮生长因子%铺片%实时定量PCR
糖尿病視網膜病變%血管內皮生長因子%鋪片%實時定量PCR
당뇨병시망막병변%혈관내피생장인자%포편%실시정량PCR
Diabetic retinopathy%Vascular endothelial growth factor%Pave piece%Real time quantitative PCR
目的 研究和探讨核因子κB (NF-κ B)在糖尿病视网膜病变中的作用.方法 首先建立糖尿病视网膜病变(DR,diabetic retinopathy)动物模型,雄性SD大鼠,链脲佐菌素(streptozotocin,STZ) 60 mg/kg腹腔注射形成糖尿病模型,1.5个月后SD大鼠玻璃体内行血管内皮生长因子(VEGF)注射造成增殖型的糖尿病视网膜病变模型.视网膜铺片使用ADP酶染色视网膜血管,实时定量PCR检测NF-κ B表达量的变化,以及凋亡信号的检测.结果 视网膜血管染色的增殖面积造模给水组为(12.0±4.1)％,而视网膜血管染色的无灌注区面积造模给NF-κ B拮抗剂组为(37.9±3.9)％.正常组大鼠的NF-κ B蛋白的灰度值为(194.2890±3.8900),而造模给水组的NF-κB蛋白的灰度值为(74.4300±4.4900),凋亡实验结果显示正常组大鼠的凋亡信号个数平均为(32.2±3.1),而造模给水组的凋亡信号个数平均为(7.4±4.2).结论 NF-κB主要是通过引起细胞凋亡而减少糖尿病视网膜病变患者视网膜血管的增殖.
目的 研究和探討覈因子κB (NF-κ B)在糖尿病視網膜病變中的作用.方法 首先建立糖尿病視網膜病變(DR,diabetic retinopathy)動物模型,雄性SD大鼠,鏈脲佐菌素(streptozotocin,STZ) 60 mg/kg腹腔註射形成糖尿病模型,1.5箇月後SD大鼠玻璃體內行血管內皮生長因子(VEGF)註射造成增殖型的糖尿病視網膜病變模型.視網膜鋪片使用ADP酶染色視網膜血管,實時定量PCR檢測NF-κ B錶達量的變化,以及凋亡信號的檢測.結果 視網膜血管染色的增殖麵積造模給水組為(12.0±4.1)％,而視網膜血管染色的無灌註區麵積造模給NF-κ B拮抗劑組為(37.9±3.9)％.正常組大鼠的NF-κ B蛋白的灰度值為(194.2890±3.8900),而造模給水組的NF-κB蛋白的灰度值為(74.4300±4.4900),凋亡實驗結果顯示正常組大鼠的凋亡信號箇數平均為(32.2±3.1),而造模給水組的凋亡信號箇數平均為(7.4±4.2).結論 NF-κB主要是通過引起細胞凋亡而減少糖尿病視網膜病變患者視網膜血管的增殖.
목적 연구화탐토핵인자κB (NF-κ B)재당뇨병시망막병변중적작용.방법 수선건립당뇨병시망막병변(DR,diabetic retinopathy)동물모형,웅성SD대서,련뇨좌균소(streptozotocin,STZ) 60 mg/kg복강주사형성당뇨병모형,1.5개월후SD대서파리체내행혈관내피생장인자(VEGF)주사조성증식형적당뇨병시망막병변모형.시망막포편사용ADP매염색시망막혈관,실시정량PCR검측NF-κ B표체량적변화,이급조망신호적검측.결과 시망막혈관염색적증식면적조모급수조위(12.0±4.1)％,이시망막혈관염색적무관주구면적조모급NF-κ B길항제조위(37.9±3.9)％.정상조대서적NF-κ B단백적회도치위(194.2890±3.8900),이조모급수조적NF-κB단백적회도치위(74.4300±4.4900),조망실험결과현시정상조대서적조망신호개수평균위(32.2±3.1),이조모급수조적조망신호개수평균위(7.4±4.2).결론 NF-κB주요시통과인기세포조망이감소당뇨병시망막병변환자시망막혈관적증식.
Objective To study the effect of nuclear factor kappa B (nf-kappa B) on diabetic retinopathy.Methods Diabetic retinopathy (DR,diabetic retinopathy) animal models were established.The SD rats intraperitoneal injected 60 cephalosporins (STZ streptozotocin) mg/kg to form diabetes models; 1.5 months from then,the SD rats were vitreously injected vascular endothelial growth factor (VEGF) to build models of proliferative diabetic retinopathy[l].Retinal blood vessels stained by ADP enzyme were used as retinal pave pieces.Real-time quantitative PCR was used to detect the e change of nf-kappa B expression quantity and apoptosis signal.Results The proliferation area of dyed retinal vessels of the model-buildingwith-water group was (12.0±4.1)％.The area without perfusion of dyed retinal blood vessels of the modelbuilding-with-nf-kappa-B-antagonist group was (37.9 ± 3.9)％.The gray value of nf-kappa B protein was (194.2890 ± 3.8900) in the normal rats and was (74.4300 ± 4.4900) in the model-building-with-water group.The results of the apoptosis experiment showed that the number of apoptotic signals was (32.2 ± 3.1) in the normal rats and was (7.4 ± 4.2) in the model-building-with-water group.Conclusions Nf-kappa B reduces the proliferation of retinal blood vessels in patients with diabetic retinopathy mainly causing cell apoptosis.
