中国药事
中國藥事
중국약사
CHINESE PHARMACEUTICAL AFFAIRS
2015年
1期
58-62
,共5页
樱花多糖%正交实验%工艺优化%抗氧化
櫻花多糖%正交實驗%工藝優化%抗氧化
앵화다당%정교실험%공예우화%항양화
cerasus serrulata polysaccharide%orthogonal design%process optimization%oxidation resistance
目的：通过正交试验优化樱花多糖的提取工艺，对樱花多糖的抗氧化功能进行了相关的研究，为樱花多糖保健功能的研究提供试验依据。方法：通过正交设计优化樱花多糖的提取工艺，用蒽酮-硫酸法测定不同提取条件下多糖的含量，并对其进行抗氧化性试验和自由基的清除试验。结果与结论：通过对试验结果进行分析，最优工艺为3 g干燥的樱花花瓣置于烧杯中，加蒸馏水240 mL，在90℃的水浴锅中提取3 h，过滤，离心，得上清液。各种浓度的樱花多糖提取液均能够有效地清除超氧阴离子自由基(O2-·),且随着浓度的增大,其清除能力逐渐增强。浓度为70.5μg·L-1多糖提取液的清除率为58.82%。
目的：通過正交試驗優化櫻花多糖的提取工藝，對櫻花多糖的抗氧化功能進行瞭相關的研究，為櫻花多糖保健功能的研究提供試驗依據。方法：通過正交設計優化櫻花多糖的提取工藝，用蒽酮-硫痠法測定不同提取條件下多糖的含量，併對其進行抗氧化性試驗和自由基的清除試驗。結果與結論：通過對試驗結果進行分析，最優工藝為3 g榦燥的櫻花花瓣置于燒杯中，加蒸餾水240 mL，在90℃的水浴鍋中提取3 h，過濾，離心，得上清液。各種濃度的櫻花多糖提取液均能夠有效地清除超氧陰離子自由基(O2-·),且隨著濃度的增大,其清除能力逐漸增彊。濃度為70.5μg·L-1多糖提取液的清除率為58.82%。
목적：통과정교시험우화앵화다당적제취공예，대앵화다당적항양화공능진행료상관적연구，위앵화다당보건공능적연구제공시험의거。방법：통과정교설계우화앵화다당적제취공예，용은동-류산법측정불동제취조건하다당적함량，병대기진행항양화성시험화자유기적청제시험。결과여결론：통과대시험결과진행분석，최우공예위3 g간조적앵화화판치우소배중，가증류수240 mL，재90℃적수욕과중제취3 h，과려，리심，득상청액。각충농도적앵화다당제취액균능구유효지청제초양음리자자유기(O2-·),차수착농도적증대,기청제능력축점증강。농도위70.5μg·L-1다당제취액적청제솔위58.82%。
Objective:To optimize the extraction process of cerasus serrulata polysaccharide by the orthogonal experiment, conduct the related research of antioxidant function of cerasus serrulata polysaccharide and provide experimental basis for the research on the healthcare function of cerasus serrulata polysaccharide. Methods:The orthogonal design was used to optimize the extraction process of polysaccharide. The anthrone-sulfuric acid method was used to determine the content of polysaccharides in different extraction conditions and the oxidation resistance test and the free-radical clearance test were conducted. Results and Conclusion:Through the analysis of the test results, the optimum process was as follows. The 3 g dried Cerasus serrulata petals were put in the beaker with 240 mL distillated water and were extracted for 3 hours in the 90 ℃water bath pot to obtain the supernatant through ifltering and centrifugation. Cerasus serrulata polysaccharide extracts of various concentrations could effectively remove superoxide anion free radicals (O2-·). Along with the increase of the concentration, its removal ability was gradually improved. The clearance rate of the polysaccharide extract at a concentration of 70.5μg·L-1 was 58.82%.
