CAJ | 학술논문

目的探讨无创远程肢体缺血联合处理对大鼠肾脏急性缺血再灌注损伤的保护及作用机制.方法 30只健康雄性SD大鼠,随机分为3组(每组10只):A组为假手术组(Sham组)、B组为缺血再灌注组(IR组)、c组为无创远程肢体缺血联合处理组(RIperC+ RIpostC组).再灌注24h测定血清中肌酐(cr)和尿素氮(BUN)含量,肾脏组织中髓过氧化物酶(MPO)活力、丙二醛(MDA)含量和超氧化物歧化酶(SOD)活力并在光镜下观察肾脏组织形态学变化.结果 B组的Cr(429.52±29.08) μmol/L、BUN(39.05± 2.23) mmol/L、MPO(7.31±1.48) U/g、MDA (3.94±0.48) nmoL/mgprot均高于A组Cr(103.91±21.45)μmol/L(P＜0.001)、BUN(12.20±1.86) mmol/L(P＜0.001)、MPO(2.25±0.89) U/g(P=0.009)、MDA(1.95±0.29) nmol/mgprot(P=0.003);而SOD(4.03±0.38) U/mgprot低于A组SOD(6.819±0.68) U/mgprot(P=0.003).c组的Cr(244.85±40.30) μmol/L(P=0.002) 、BUN(23.48±1.80) mmol/L(P＜0.001)、MPO(3.65±0.73) U/g(P =0.045)、MDA(2.19±0.31) nmol/mgprot(P=0.006)均低于B组,而SOD(5.71±0.30) U/mgprot(P=0.003)高于B组.A组组织形态基本正常,c组组织形态学改变较B组明显减轻.结论无创远程肢体缺血联合处理对肾脏急性缺血再灌注损伤有显著保护作用.其保护作用可能通过对肢体短暂的缺血再灌注激发了机体内源性的抗氧化能力,从而达到减轻肾脏的急性缺血再灌注损伤.
목적 탐토무창원정지체결혈연합처리대대서신장급성결혈재관주손상적보호급작용궤제.방법 30지건강웅성SD대서,수궤분위3조(매조10지):A조위가수술조(Sham조)、B조위결혈재관주조(IR조)、c조위무창원정지체결혈연합처리조(RIperC+ RIpostC조).재관주24h측정혈청중기항(cr)화뇨소담(BUN)함량,신장조직중수과양화물매(MPO)활력、병이철(MDA)함량화초양화물기화매(SOD)활력병재광경하관찰신장조직형태학변화.결과 B조적Cr(429.52±29.08) μmol/L、BUN(39.05± 2.23) mmol/L、MPO(7.31±1.48) U/g、MDA (3.94±0.48) nmoL/mgprot균고우A조Cr(103.91±21.45)μmol/L(P＜0.001)、BUN(12.20±1.86) mmol/L(P＜0.001)、MPO(2.25±0.89) U/g(P=0.009)、MDA(1.95±0.29) nmol/mgprot(P=0.003);이SOD(4.03±0.38) U/mgprot저우A조SOD(6.819±0.68) U/mgprot(P=0.003).c조적Cr(244.85±40.30) μmol/L(P=0.002) 、BUN(23.48±1.80) mmol/L(P＜0.001)、MPO(3.65±0.73) U/g(P =0.045)、MDA(2.19±0.31) nmol/mgprot(P=0.006)균저우B조,이SOD(5.71±0.30) U/mgprot(P=0.003)고우B조.A조조직형태기본정상,c조조직형태학개변교B조명현감경.결론 무창원정지체결혈연합처리대신장급성결혈재관주손상유현저보호작용.기보호작용가능통과대지체단잠적결혈재관주격발료궤체내원성적항양화능력,종이체도감경신장적급성결혈재관주손상.
Objectives To investigate the noninvasive remote ischemic limb perconditioning and postconditioning combined on acute renal ischemia-reperfusion injury and its mechanism in rats.Results 30 healthy male SD rats were randomly divided into three groups (n =10):A is sham operation group (Sham group),B is ischemia -reperfusion group (IR),C is noninvasive remote ischemic limb perconditioning and postconditioning combined treatment group (RIperC + RIpostC group).After 24h reperfusion,serum creatinine (Cr) and urea nitrogen (BUN) levels,kidney tissue myeloperoxidase (MPO) activity,malondialdehyde (MDA) content and superoxide dismutase (SOD) activity were measured and the light microscopy observed renal histological changes.Methods These indicators in group B Cr(429.52 ±29.08) μmol/L、BUN(39.05 ±2.23) mmol/L、MPO(7.31 ± 1.48) U/g、MDA (3.94± 0.48) nmol/mgprot were higher than group A Cr(103.91 ± 21.45) μ mol/L (P ＜ 0.001)、BUN (12.20 ± 1.86) mmol/L(p ＜0.001)、MPO(2.25 ±0.89) U/g(P =0.009)、MDA(1.95 ±0.29) nmol/mgprot (p =0.003) while SOD(4.03 ±0.38) U/mgprut lower in group A SOD(6.819 ±0.68) U/mgprot(P =0.003) ; group C Cr(244.85 ± 40.30) μmol/L(p =0.002) 、BUN(23.48 ± 1.80) mmol/L(p ＜0.001) 、MPO(3.65 ±0.73) U/g(P =0.045)、MDA(2.19 ±0.31) nmol/mgprot(p =0.006) were lower than group B(P ＜0.05),while SOD SOD(5.71 ±0.30) U/mgprot(P =0.003) higher than in group B.Group A is normal morphology,group C is more significantly reduced than group B in morphological changes.Conclusions The noninvasive remote ischemic limb perconditioning and postconditioning combined on acute renal ischemia-reperfusion injury have significant protective effect.Through its protective effect may be transient limb ischemia-reperfusion stimulate e-ndogenous antioxidant capacity,so as to alleviate acute renal ischemia-reperfusion injury.