国际肿瘤学杂志
國際腫瘤學雜誌
국제종류학잡지
JOURNAL OF INTERNATIONAL ONCOLOGY
2014年
12期
946-949
,共4页
基因,p16%宫颈肿瘤%胰岛素样生长因子Ⅱ mRNA结合蛋白3
基因,p16%宮頸腫瘤%胰島素樣生長因子Ⅱ mRNA結閤蛋白3
기인,p16%궁경종류%이도소양생장인자Ⅱ mRNA결합단백3
Genes,p16%Uterine cervical neoplasms%Insulin-like growth factor Ⅱ mRNA-binding protein 3
目的 检测胰岛素样生长因子ⅡmRNA结合蛋白3(IMP3)与p16在宫颈癌的表达,探讨其临床意义.方法 采用免疫组织化学Envision二步法检测67例宫颈癌、58例宫颈上皮内瘤变及15例正常宫颈组织中IMP3及p16的表达情况.结果 IMP3在宫颈癌组织细胞质中表达,阳性表达率85.07％(57/67),显著高于宫颈上皮内瘤变12.07％(7/58; x2 =66.32,P＜0.05)及正常宫颈组织的阳性表达率0(0/15;x2 =44.38,P＜0.05).IMP3在宫颈腺癌及宫颈鳞状细胞癌中均呈高表达,两者差异无统计学意义(x2=0.35,P＞0.05).p16在宫颈癌组织细胞质和细胞核均有表达,阳性表达率为83.58％(56/67),显著高于宫颈上皮内瘤变41.37％(24/58;x2 =24.85,P＜0.05)及正常宫颈组织的阳性表达率0(0/15;x2 =42.61,P＜0.05).结论 IMP3与p16在宫颈癌中高表达,可能作为预测宫颈癌转移潜能及预测宫颈上皮内瘤变进展为宫颈浸润癌的指标.
目的 檢測胰島素樣生長因子ⅡmRNA結閤蛋白3(IMP3)與p16在宮頸癌的錶達,探討其臨床意義.方法 採用免疫組織化學Envision二步法檢測67例宮頸癌、58例宮頸上皮內瘤變及15例正常宮頸組織中IMP3及p16的錶達情況.結果 IMP3在宮頸癌組織細胞質中錶達,暘性錶達率85.07％(57/67),顯著高于宮頸上皮內瘤變12.07％(7/58; x2 =66.32,P＜0.05)及正常宮頸組織的暘性錶達率0(0/15;x2 =44.38,P＜0.05).IMP3在宮頸腺癌及宮頸鱗狀細胞癌中均呈高錶達,兩者差異無統計學意義(x2=0.35,P＞0.05).p16在宮頸癌組織細胞質和細胞覈均有錶達,暘性錶達率為83.58％(56/67),顯著高于宮頸上皮內瘤變41.37％(24/58;x2 =24.85,P＜0.05)及正常宮頸組織的暘性錶達率0(0/15;x2 =42.61,P＜0.05).結論 IMP3與p16在宮頸癌中高錶達,可能作為預測宮頸癌轉移潛能及預測宮頸上皮內瘤變進展為宮頸浸潤癌的指標.
목적 검측이도소양생장인자ⅡmRNA결합단백3(IMP3)여p16재궁경암적표체,탐토기림상의의.방법 채용면역조직화학Envision이보법검측67례궁경암、58례궁경상피내류변급15례정상궁경조직중IMP3급p16적표체정황.결과 IMP3재궁경암조직세포질중표체,양성표체솔85.07％(57/67),현저고우궁경상피내류변12.07％(7/58; x2 =66.32,P＜0.05)급정상궁경조직적양성표체솔0(0/15;x2 =44.38,P＜0.05).IMP3재궁경선암급궁경린상세포암중균정고표체,량자차이무통계학의의(x2=0.35,P＞0.05).p16재궁경암조직세포질화세포핵균유표체,양성표체솔위83.58％(56/67),현저고우궁경상피내류변41.37％(24/58;x2 =24.85,P＜0.05)급정상궁경조직적양성표체솔0(0/15;x2 =42.61,P＜0.05).결론 IMP3여p16재궁경암중고표체,가능작위예측궁경암전이잠능급예측궁경상피내류변진전위궁경침윤암적지표.
Objective To detect the expression of insulin-like growth factor Ⅱ mRNA-binding protein 3 (IMP3) and p16 in cervical carcinoma and investigate their clinical significance.Methods Immunohistochemical Envision method was used to detect the expression of IMP3 and p16 proteins in 67 cases of carcinoma of the uterine cervix,58 cases of cervical intraepithelial neoplasia (CIN) and 15 cases of normal cervical epithelium.Results IMP3 was mainly dedected in the cytoplasm,whose positive expression rate was 85.07％ (57/67) in cervical carcinoma,12.07％ (7/58) in CIN (x2 =66.32,P ＜ 0.05) and 0 (0/15) in cervical epithelium (x2 =44.38,P ＜ 0.05).No significance variations of the expression of IMP3 were observed in cervical adenocarcinoma and squamous cell carcinoma (x2 =0.35,P ＞ 0.05).p16 was found both in nucleus and cytoplasm of cervical carcinoma,whose expression rate was 83.58％ (56/67),higher than 41.37％ (24/58) in CIN (x2 =24.85,P ＜ 0.05) and 0 (0/15) in cervical epithelium (x2 =42.61,P ＜ 0.05).Conclusion Expression of IMP3 and p16 is high in cervical carcinoma,maybe can be used as a potential biomarker of metastasis and progression.
