CAJ | 학술논문

目的探讨老年喉癌患者外周血中CD4+ CD25+叉状头转录因子p3(Foxp3)+调节性T细胞(Treg)和CD4+白细胞介素(IL)-17+辅助性T细胞17(Th17)的表达特点及其与老年喉癌患者病程进展的关系.方法选取2013年1月至2014年6月在解放军第180医院耳鼻喉科确诊为喉癌的老年患者60例,其中伴颈部淋巴结转移28例,无颈部淋巴结转移32例,另取同期无喉部疾病的健康人30例作为对照.分别抽取老年喉癌患者及正常对照者外周血,以流式细胞术检测各组人群外周血中Treg和Th17细胞表达水平,实时定量聚合酶链反应检测外周血单核细胞(PBMC)中Foxp3、维甲酸相关核孤儿受体(RORγt)基因表达变化.结果与对照组相比,无淋巴结转移老年喉癌患者Treg细胞百分比(t=9.817,P=0.018)、Th17细胞百分比(t=12.120,P=0.009)、Foxp3(t=6.090,P=0.023)和RORγt(t=7.130,P=0.028)表达水平均有所升高.伴淋巴结转移老年喉癌患者与无淋巴结转移老年喉癌患者相比,Treg细胞百分比(t=9.070,P=0.014)、Th17细胞百分比(t=12.140,P=0.009)和Foxp3(t=10.130,P=0.009)、RORγt(t=13.070,P=0.008)表达水平均显著升高,差异均有统计学意义.而伴淋巴结转移老年喉癌患者Treg/Th17和Foxp3/RORγt比显著低于无淋巴结转移老年喉癌患者(2.401±0.614 vs 3.763±0.959;0.401±0.075 vs 0.563±0.091),差异有统计学意义(t=9.070,P=0.014;t=11.140,P=0.007).结论老年喉癌患者中Treg细胞和Th17细胞表达失衡程度与老年性喉癌患者病情的进展呈正相关.因此,监测及纠正老年喉癌患者Treg和Th17细胞水平对于本疾病的辅助诊断、治疗及预后有积极作用.
목적 탐토노년후암환자외주혈중CD4+ CD25+차상두전록인자p3(Foxp3)+조절성T세포(Treg)화CD4+백세포개소(IL)-17+보조성T세포17(Th17)적표체특점급기여노년후암환자병정진전적관계.방법 선취2013년1월지2014년6월재해방군제180의원이비후과학진위후암적노년환자60례,기중반경부림파결전이28례,무경부림파결전이32례,령취동기무후부질병적건강인30례작위대조.분별추취노년후암환자급정상대조자외주혈,이류식세포술검측각조인군외주혈중Treg화Th17세포표체수평,실시정량취합매련반응검측외주혈단핵세포(PBMC)중Foxp3、유갑산상관핵고인수체(RORγt)기인표체변화.결과 여대조조상비,무림파결전이노년후암환자Treg세포백분비(t=9.817,P=0.018)、Th17세포백분비(t=12.120,P=0.009)、Foxp3(t=6.090,P=0.023)화RORγt(t=7.130,P=0.028)표체수평균유소승고.반림파결전이노년후암환자여무림파결전이노년후암환자상비,Treg세포백분비(t=9.070,P=0.014)、Th17세포백분비(t=12.140,P=0.009)화Foxp3(t=10.130,P=0.009)、RORγt(t=13.070,P=0.008)표체수평균현저승고,차이균유통계학의의.이반림파결전이노년후암환자Treg/Th17화Foxp3/RORγt비현저저우무림파결전이노년후암환자(2.401±0.614 vs 3.763±0.959;0.401±0.075 vs 0.563±0.091),차이유통계학의의(t=9.070,P=0.014;t=11.140,P=0.007).결론 노년후암환자중Treg세포화Th17세포표체실형정도여노년성후암환자병정적진전정정상관.인차,감측급규정노년후암환자Treg화Th17세포수평대우본질병적보조진단、치료급예후유적겁작용.
Objective To investigate the relationship of the expression characteristics of CD4 + CD25 + Foxp3 + Treg cells and CD4 + IL-17 + Th17 cells and the progress of the elderly patients with laryngeal cancer.Methods Sixty elderly patients diagnosed as laryngeal cancer in our hospital from January 2013 to June 2014 were chosen.Among them,28 cases had lymph node metastasis and 32 without.Thirty cases without laryngeal diseases were chosen as control.The peripheral blood of elderly patients with laryngeal cancer and normal controls was extracted.The expression levels of Treg and Th17 cells in all groups were detected with flow cytometry,and the expression changes of Foxp3,RORγt in peripheral blood mononuclear cell (PBMC) were detected with real time quantitative PCR.Results Compared with the control group,CD4 + CD25 + Foxp3 + Treg and CD4 + IL-17 + Th17 cell percentage,Treg and Th17 cells specific transcription factor Foxp3,RORγt expression levels in elderly laryngeal cancer patients without lymph node metastasis were significantly higher (t=9.817,P=0.018; t=12.120,P=0.009; t=6.090,P=0.023; t=7.130,P=0.028).Compared with elderly laryngeal cancer patients without lymph node metastasis,Treg and Th17 cell percentage and Foxp3,RORγt expression levels were significantly increased (t =9.070,P =0.014; t =12.140,P =0.009; t =10.130,P =0.009 ; t =13.070,P =0.008),and the ratios of Th17/Treg and Foxp3/RORγt were significantly lower (2.401 ± 0.614 vs 3.763 ± 0.959 ; 0.401 ± 0.075 vs 0.563 ± 0.091 ; t =9.070,P =0.014 ; t =11.140,P =0.007) in elderly laryngeal cancer patients with lymph node metastasis.Conclusion In elderly patients with laryngeal cancer,Treg cells and Th17 cells imbalance is positively correlated to disease progression.Therefore,monitoring and correcting the expression levels of Treg and Th17 cells may be important for the diagnosis,treatment and prognosis of the elderly patients with laryngeal cancer.