国际呼吸杂志
國際呼吸雜誌
국제호흡잡지
INTERNATIONAL JOURNAL OF RESPIRATION
2014年
24期
1851-1854
,共4页
地塞米松%基质金属蛋白酶%三维立体培养%细胞因子%肿瘤坏死因子α%白介素1β%肺癌
地塞米鬆%基質金屬蛋白酶%三維立體培養%細胞因子%腫瘤壞死因子α%白介素1β%肺癌
지새미송%기질금속단백매%삼유입체배양%세포인자%종류배사인자α%백개소1β%폐암
Dexamethasone%Matrix metalloproteinase%Three-dimensional culture%Cytokine%Tumor necrosis factor-α%Interleukin-1 β%Lung cancer
目的 在肺癌H460细胞的三维立体培养过程中,直接观察细胞因子[肿瘤坏死因子α(TNF-α)和IL-1β]对肺癌H460细胞关于基质金属蛋白酶-2(MMP-2)和MMP-9表达的诱导作用及地塞米松的干预作用.方法 在肺癌H460细胞三维立体培养过程中加入细胞因子TNF-α和IL-1β来诱导MMP的表达,同时加入地塞米松干预MMP-2和MMP-9的表达;用明胶酶谱法测定MMP-2、MMP-9,同时在培养5d后测定胶原含量大小.结果 TNF-α和IL-1β诱导培养在立体胶原内的肺癌H460细胞产生大量MMP-2和MMP-9,并促进其活化,引起大量胶原降解,地塞米松抑制了MMP-2及MMP-9的表达及活化,进而抑制了胶原的降解,对抗了细胞因子TNF-α和IL-1β对胶原的降解作用.结论 细胞因子TNF-α和IL-1β可诱导MMP的表达,MMP可直接降解肺内胶原,进而促进肺癌的侵袭或转移;地塞米松通过抑制MMP的表达与活化对抗MMP对肺组织的破坏作用,进而抑制了肺癌的侵袭与转移.
目的 在肺癌H460細胞的三維立體培養過程中,直接觀察細胞因子[腫瘤壞死因子α(TNF-α)和IL-1β]對肺癌H460細胞關于基質金屬蛋白酶-2(MMP-2)和MMP-9錶達的誘導作用及地塞米鬆的榦預作用.方法 在肺癌H460細胞三維立體培養過程中加入細胞因子TNF-α和IL-1β來誘導MMP的錶達,同時加入地塞米鬆榦預MMP-2和MMP-9的錶達;用明膠酶譜法測定MMP-2、MMP-9,同時在培養5d後測定膠原含量大小.結果 TNF-α和IL-1β誘導培養在立體膠原內的肺癌H460細胞產生大量MMP-2和MMP-9,併促進其活化,引起大量膠原降解,地塞米鬆抑製瞭MMP-2及MMP-9的錶達及活化,進而抑製瞭膠原的降解,對抗瞭細胞因子TNF-α和IL-1β對膠原的降解作用.結論 細胞因子TNF-α和IL-1β可誘導MMP的錶達,MMP可直接降解肺內膠原,進而促進肺癌的侵襲或轉移;地塞米鬆通過抑製MMP的錶達與活化對抗MMP對肺組織的破壞作用,進而抑製瞭肺癌的侵襲與轉移.
목적 재폐암H460세포적삼유입체배양과정중,직접관찰세포인자[종류배사인자α(TNF-α)화IL-1β]대폐암H460세포관우기질금속단백매-2(MMP-2)화MMP-9표체적유도작용급지새미송적간예작용.방법 재폐암H460세포삼유입체배양과정중가입세포인자TNF-α화IL-1β래유도MMP적표체,동시가입지새미송간예MMP-2화MMP-9적표체;용명효매보법측정MMP-2、MMP-9,동시재배양5d후측정효원함량대소.결과 TNF-α화IL-1β유도배양재입체효원내적폐암H460세포산생대량MMP-2화MMP-9,병촉진기활화,인기대량효원강해,지새미송억제료MMP-2급MMP-9적표체급활화,진이억제료효원적강해,대항료세포인자TNF-α화IL-1β대효원적강해작용.결론 세포인자TNF-α화IL-1β가유도MMP적표체,MMP가직접강해폐내효원,진이촉진폐암적침습혹전이;지새미송통과억제MMP적표체여활화대항MMP대폐조직적파배작용,진이억제료폐암적침습여전이.
Objective To investigate the effects of dexamethasone on cytokines [tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β)] to induce expressions of matrix metalloproteinase-2 (MMP-2) and MMP-9 in lung cancer H460 cells.Methods Human lung cancer H460 cells were cultured in three-dimensional collagen gels.TNF-α and IL-1β were added to induce expressions of MMP-2 and MMP-9 in lung cancer H460 cells,and dexamethasone was added to the culture media at the same time.The expressions of MMP-2 and MMP-9 were detected by gelatin zymography.Collagen content was determined on the 5th day.Results TNF-α and IL-1β induced lung cancer H460 cells to produce MMP-2 and MMP-9 in latent forms and activated MMP,resulting in generously collagen degradation.But dexamethasone inhibited the effect of cytokines (TNF-a and IL-1β) to induce productions of MMP-2 and MMP-9.Conclusions TNF-α and IL-1β may induce production and activation of MMP-2 and MMP-9,and MMP may be involved in the lung tissue destruction in the invasion and metastasis of lung cancer,however,dexamethasone may inhibit the effect of cytokine in the lung tissue destruction in the invasion and metastasis of lung cancer.
