CAJ | 학술논문

目的：探讨乌司他丁对人口腔表皮样癌细胞系KB的转移和侵袭能力的影响。方法：分别通过划痕实验、Transwell小室侵袭实验探讨乌司他丁对人口腔表皮样癌细胞系KB的转移和侵袭的影响；明胶酶谱实验研究乌司他丁对人口腔表皮样癌细胞系KB分泌活性MMP-9的影响。结果：划痕实验结果显示：与对照组转移细胞数(130.22±14.99)相比，乌司他丁处理后的KB细胞(69.78±11.79)转移能力明显受到抑制( P<0.05)；Transwell小室侵袭结果显示，乌司他丁治疗组 KB细胞侵袭的细胞数目(223.44±28.54)明显低于阴性对照组(501.11±30.75)，(P<0.05)；明胶酶谱结果显示，乌司他丁处理后的KB细胞活性MMP-9的表达明显降低( P<0.05)。结论：乌司他丁可能通过抑制MMP-9的活性从而抑制口腔癌细胞的转移和侵袭。
목적：탐토오사타정대인구강표피양암세포계KB적전이화침습능력적영향。방법：분별통과화흔실험、Transwell소실침습실험탐토오사타정대인구강표피양암세포계KB적전이화침습적영향；명효매보실험연구오사타정대인구강표피양암세포계KB분비활성MMP-9적영향。결과：화흔실험결과현시：여대조조전이세포수(130.22±14.99)상비，오사타정처리후적KB세포(69.78±11.79)전이능력명현수도억제( P<0.05)；Transwell소실침습결과현시，오사타정치료조 KB세포침습적세포수목(223.44±28.54)명현저우음성대조조(501.11±30.75)，(P<0.05)；명효매보결과현시，오사타정처리후적KB세포활성MMP-9적표체명현강저( P<0.05)。결론：오사타정가능통과억제MMP-9적활성종이억제구강암세포적전이화침습。
Objective:To study the effect of Ulinastatin on cell migration and invasion in human oral epidermoid carcinoma cell line (OECC) KB.Methods:Effect of Ulinastatin on migration and invasion in OECC cell line KB was determined by wound healing assay and Transwell chamber invasion assay, respectively;Gelatin zymography experiments was used to detect the effect of Ulinastatin on MMP-9 expression in OECC cell line KB. Results:Wound healing assay showed that KB cell migration was significantly suppressed by Ulinastatin (69.78 ±11.79) compared with control group(130.22±14.99) (P<0.05); Transwell chamber invasion assay showed that invaded cell number in Ulinastatin group (223.44±28.54) was significantly lower compared to the negative control group(501.11±30.75), P<0.05;Gelatin zymography experimental results showed that MMP-9 expression in KB cell was significantly lower after treatment of Ulinastatin (P<0.05).Conclusion: Ulinastatin may inhibits migration and invasion of KB cell via inhibiting the activity of MMP-9.