CAJ | 학술논문

目的：研究 miR-21反义寡核苷酸（anti-miR-21 oligonucleotide， AMO）对地西他滨（decitabine， DCA）抗白血病效应的影响及可能机制。方法：将AMO和无义寡核苷酸（ scramble oligonucleotide ， SCR）通过脂质体转染导入HL-60细胞，实时荧光定量PCR（ real-time PCR）验证转染效率，再分别与DCA 0.5、2.0和4.0μmol／L作用48 h。 Real-time PCR分别检测人周期节律蛋白3（hPer3） mRNA表达，Annexin V／PI法检测凋亡，流式细胞术检测CD117和CD11b平均荧光强度（MFI）。结果： AMO转染组miR-21表达（0.35±0.07）低于空白组（0.71±0.07）和SCR转染组（0.66±0.05），差异有统计学意义（P＜0.05）。 AMO转染组的HL-60细胞DCA的IC50低于空白组和SCR转染组（P＜0.01）。同一浓度下，AMO组的早期凋亡率、CD11b的MFI和hPer3 mRNA均高于同一浓度药物作用的空白组和SCR组，CD117 MFI均低于同一浓度药物作用的空白组和SCR组，差异均具有统计学意义（P＜0.01）。结论：AMO能显著促进DCA体外抗白血病效应，其机制可能与其协助激活hPer3的表达有关。
목적：연구 miR-21반의과핵감산（anti-miR-21 oligonucleotide， AMO）대지서타빈（decitabine， DCA）항백혈병효응적영향급가능궤제。방법：장AMO화무의과핵감산（ scramble oligonucleotide ， SCR）통과지질체전염도입HL-60세포，실시형광정량PCR（ real-time PCR）험증전염효솔，재분별여DCA 0.5、2.0화4.0μmol／L작용48 h。 Real-time PCR분별검측인주기절률단백3（hPer3） mRNA표체，Annexin V／PI법검측조망，류식세포술검측CD117화CD11b평균형광강도（MFI）。결과： AMO전염조miR-21표체（0.35±0.07）저우공백조（0.71±0.07）화SCR전염조（0.66±0.05），차이유통계학의의（P＜0.05）。 AMO전염조적HL-60세포DCA적IC50저우공백조화SCR전염조（P＜0.01）。동일농도하，AMO조적조기조망솔、CD11b적MFI화hPer3 mRNA균고우동일농도약물작용적공백조화SCR조，CD117 MFI균저우동일농도약물작용적공백조화SCR조，차이균구유통계학의의（P＜0.01）。결론：AMO능현저촉진DCA체외항백혈병효응，기궤제가능여기협조격활hPer3적표체유관。
AIM:To investigate the role of anti-miR-21 oligonucleotide ( AMO) in the anti-leukemic activity of decitabine (DCA) in vitro.METHODS:AMO and scramble oligonucleotide (SCR) were constructed and transfected into HL-60 cells.The miR-21 expression was analyzed by real-time PCR to identify the transfection efficiency .The cells were treated with DCA at gradient concentrations (0.5, 2.0 and 4.0 μmol/L) for 48 h.The mRNA expression of human period circadian protein 3 (hPer3) was detected by real-time PCR.The early apoptotic rates were determined by flow cy-tometry with Annexin V/PI staining.Mean fluorescence intensities ( MFI) of CD117 and CD11b were also measured by flow cytometry.RESULTS:The miR-21 relative expression level in AMO group was significantly lower than that in blank group and SCR group (P<0.01).IC50 of DCA in AMO group was significantly lower than that in blank group and SCR group (P<0.01).With the same concentration of DCA, the early apoptotic rate, the mRNA expression of hPer3 and the MFI of CD11b in AMO group were significantly higher than those in blank group and SCR group (P<0.01).The MFI of CD117 in AMO group were significantly lower than those in blank group and SCR group ( P<0.01 ) .CONCLUSION:Activation of hPer3 expression plays an important role in enhanced anti-leukemic activity of decitabine by AMO in vitro.