牙体牙髓牙周病学杂志
牙體牙髓牙週病學雜誌
아체아수아주병학잡지
CHINESE JOURNAL OF CONSERVATIVE DENTISTRY
2015年
1期
15-19
,共5页
冯小倩%张纲%张慧宇%冯晓丹%张萍%谭颖徽
馮小倩%張綱%張慧宇%馮曉丹%張萍%譚穎徽
풍소천%장강%장혜우%풍효단%장평%담영휘
牙周炎%大鼠%组胺%组胺受体Ⅰ(HR1)
牙週炎%大鼠%組胺%組胺受體Ⅰ(HR1)
아주염%대서%조알%조알수체Ⅰ(HR1)
periodontitis%rat%histamine%histamine receptor 1
目的:研究组胺及组胺受体1(HR1)在牙周炎发病机制中的作用。方法:将40只雄性SD大鼠随机分为实验组和对照组(每组20只),其中实验组采用正畸丝结扎法建立牙周炎模型,对照组不作任何处理;建模8周后,分别采用ELISA法检测各组龈沟液中组胺含量,荧光定量PCR法检测各组牙龈组织中HR1 mRNA的表达水平。结果:牙周炎组龈沟液中的组胺浓度(35.47±3.908)μg/L 高于正常对照组(19.77± 3.832)μg/L(P<0.05);牙周炎组牙龈组织中的HR1-mRNA表达水平低于正常对照组(P<0.05)。结论:组胺及HR1对牙周炎的发病均有一定调控作用。
目的:研究組胺及組胺受體1(HR1)在牙週炎髮病機製中的作用。方法:將40隻雄性SD大鼠隨機分為實驗組和對照組(每組20隻),其中實驗組採用正畸絲結扎法建立牙週炎模型,對照組不作任何處理;建模8週後,分彆採用ELISA法檢測各組齦溝液中組胺含量,熒光定量PCR法檢測各組牙齦組織中HR1 mRNA的錶達水平。結果:牙週炎組齦溝液中的組胺濃度(35.47±3.908)μg/L 高于正常對照組(19.77± 3.832)μg/L(P<0.05);牙週炎組牙齦組織中的HR1-mRNA錶達水平低于正常對照組(P<0.05)。結論:組胺及HR1對牙週炎的髮病均有一定調控作用。
목적:연구조알급조알수체1(HR1)재아주염발병궤제중적작용。방법:장40지웅성SD대서수궤분위실험조화대조조(매조20지),기중실험조채용정기사결찰법건립아주염모형,대조조불작임하처리;건모8주후,분별채용ELISA법검측각조간구액중조알함량,형광정량PCR법검측각조아간조직중HR1 mRNA적표체수평。결과:아주염조간구액중적조알농도(35.47±3.908)μg/L 고우정상대조조(19.77± 3.832)μg/L(P<0.05);아주염조아간조직중적HR1-mRNA표체수평저우정상대조조(P<0.05)。결론:조알급HR1대아주염적발병균유일정조공작용。
AIM:To investigate the expression of histamine and histamine receptor 1 (HR1 )in rat gingival crevicular fluid (GCF)and gingival tissues.METHODS:40 adult SD rats were randomly divided into 2 groups(n=20):The periodontitis models were established by ligation technique and the rats in control group received no treat-ment.After 8 weeks,Histamine in GCF was detected by ELISA.HRI mRNA expression in gingival tissues was detec-ted by QT-PCR.RESULTS:The level of histamine in GCF in the periodontitis group (35.47 ±3.908)μg/L was high-er than that in control group (19.77 ±3.832)μg/L(P<0.05).HRI mRNA in gingival tissues of periodontitis group was lower than that of the control group (P<0.05).CONCLUSION:Upregulation of histamine in GCF and downregu-lation of HRI in gingival tissues may play important roles in pathogenesis of periodontitis.