牙体牙髓牙周病学杂志
牙體牙髓牙週病學雜誌
아체아수아주병학잡지
CHINESE JOURNAL OF CONSERVATIVE DENTISTRY
2015年
1期
8-14
,共7页
种植体%微沟槽%人牙龈成纤维细胞%整联蛋白
種植體%微溝槽%人牙齦成纖維細胞%整聯蛋白
충식체%미구조%인아간성섬유세포%정련단백
implant%microgroove%human gingival fibroblasts%intergrin
目的:研究微沟槽形貌对人牙龈成纤维细胞(HGF)整联蛋白intergrin α5、integrn β1表达的影响。方法:用光刻技术制作沟槽宽度分别为15、30、60μm,沟槽深度分别为5、10μm(沟槽间隔分别等于各相应宽度)的微沟槽形貌,分别命名为T15/5、T15/10、T30/5、T30/10、T60/5、T60/10,光滑钛(T0)表面作为对照组;分别测量各组材料表面的微沟槽形貌及亲水性,用Real time-PCR和Western blotting 检测HGF在各组材料表面培养3 d时的intergrin α5和β1 mRNA及蛋白的表达水平。结果:T60/5表面亲水性最好,T15/10疏水性最大并明显大于光滑组T0(P<0.05);intergrin α5 mRNA表达水平以T60/10组最高,而T15/10时最低,各组间两两相比P<0.05;沟槽宽度较小时(T15、T30),若深度增加则可减少intergrin α5 mRNA的表达,而沟槽宽度较大时(T60),若深度增加则可促进intergrin α5 mRNA的表达;intergrin β1 mRNA的表达水平以T60/10组最高,与其他各组相比P<0.05;其余3组间比较均P>0.05,intergrin α5、intergrin β1蛋白的表达趋势与其mRNA的表达基本一致。结论:intergrin α5和β1表达随着沟槽宽度增大而增加,深度对intergrin α5表达的影响与沟槽的宽度大小相关,沟槽宽度和深度对intergrin β1表达的影响程度低于intergrin α5。
目的:研究微溝槽形貌對人牙齦成纖維細胞(HGF)整聯蛋白intergrin α5、integrn β1錶達的影響。方法:用光刻技術製作溝槽寬度分彆為15、30、60μm,溝槽深度分彆為5、10μm(溝槽間隔分彆等于各相應寬度)的微溝槽形貌,分彆命名為T15/5、T15/10、T30/5、T30/10、T60/5、T60/10,光滑鈦(T0)錶麵作為對照組;分彆測量各組材料錶麵的微溝槽形貌及親水性,用Real time-PCR和Western blotting 檢測HGF在各組材料錶麵培養3 d時的intergrin α5和β1 mRNA及蛋白的錶達水平。結果:T60/5錶麵親水性最好,T15/10疏水性最大併明顯大于光滑組T0(P<0.05);intergrin α5 mRNA錶達水平以T60/10組最高,而T15/10時最低,各組間兩兩相比P<0.05;溝槽寬度較小時(T15、T30),若深度增加則可減少intergrin α5 mRNA的錶達,而溝槽寬度較大時(T60),若深度增加則可促進intergrin α5 mRNA的錶達;intergrin β1 mRNA的錶達水平以T60/10組最高,與其他各組相比P<0.05;其餘3組間比較均P>0.05,intergrin α5、intergrin β1蛋白的錶達趨勢與其mRNA的錶達基本一緻。結論:intergrin α5和β1錶達隨著溝槽寬度增大而增加,深度對intergrin α5錶達的影響與溝槽的寬度大小相關,溝槽寬度和深度對intergrin β1錶達的影響程度低于intergrin α5。
목적:연구미구조형모대인아간성섬유세포(HGF)정련단백intergrin α5、integrn β1표체적영향。방법:용광각기술제작구조관도분별위15、30、60μm,구조심도분별위5、10μm(구조간격분별등우각상응관도)적미구조형모,분별명명위T15/5、T15/10、T30/5、T30/10、T60/5、T60/10,광활태(T0)표면작위대조조;분별측량각조재료표면적미구조형모급친수성,용Real time-PCR화Western blotting 검측HGF재각조재료표면배양3 d시적intergrin α5화β1 mRNA급단백적표체수평。결과:T60/5표면친수성최호,T15/10소수성최대병명현대우광활조T0(P<0.05);intergrin α5 mRNA표체수평이T60/10조최고,이T15/10시최저,각조간량량상비P<0.05;구조관도교소시(T15、T30),약심도증가칙가감소intergrin α5 mRNA적표체,이구조관도교대시(T60),약심도증가칙가촉진intergrin α5 mRNA적표체;intergrin β1 mRNA적표체수평이T60/10조최고,여기타각조상비P<0.05;기여3조간비교균P>0.05,intergrin α5、intergrin β1단백적표체추세여기mRNA적표체기본일치。결론:intergrin α5화β1표체수착구조관도증대이증가,심도대intergrin α5표체적영향여구조적관도대소상관,구조관도화심도대intergrin β1표체적영향정도저우intergrin α5。
AIM:To investigate the effects of microgroove surface morphology on the expression of intergrinα5 and intergrin β1 in human gingival fibroblasts(HGFs).METHODS:Microgroove titanium surface was fabricated by photolithography with parallel grooves:15 μm,30 μm or 60 μm in width and 5 μm or 10 μm in depth.The combi-nations of groove width and depth were hereafter denoted as T15/5,T15/10,T30/5,T30/10,T60/5 and T60/10. Smooth titanium surface(T0)was used as the control.Surface topography and hydrophilicity were detected.HGFs were cultured on the microgroove surfaces for 3 days.Intergrin α5 and intergrin β1 expression was examined by Real-time PCR and western blotting.RESULTS:T60/5 microgroove showed the highest surface hydrophilicity.However, T15/10 had the highest surface hydrophobicity,even higher than T0 (P<0.05 ).T60/10 group showed the highest expression of intergrin α5 and intergrin β1 mRNA,and in T15/10 group the lowest (P<0.05).In groups with lower
<br> microgroove depth (T15,T30),expression of intergrin α5 decreased with the increase of microgroove depth;while in groups with higher microgroove depth (T60 ),expression of intergrin α5 mRNA increased with the increase of micro-groove depth.T60/10 group showed the highest expression of intergrin β1 mRNA (P <0.05 ),among the other 3 groups,P>0.05.The expression of intergrin α5 and β1 protein showed similar pattern with mRNA expression. CONCLUSION:The effects of microgroove depth on intergrinα5 and interginβ1 depended on micro-groove width.Mi-crogroove width and depth showed less effect on intergrin β1 mRNA and protein when compared to that on intergrin α5 .