CAJ | 학술논문

利用常压室温等离子体诱变技术对产葡萄糖氧化酶的黑曲霉菌株进行诱变处理，通过平板筛选以及摇瓶复筛选出8株酶活较高的菌株，其中产酶活力最高的突变株为PCTC-8，酶活达到14．36 U／mL，较初始菌株的酶活提高了117．25％。然后在优化培养基的基础之上通过单因素实验对诱变菌株的发酵条件进行优化，最终确定最优的发酵条件为：接种量10％，装液量30 mL，种龄24 h，发酵时间48 h，转速225 r／min，在此条件下最高酶活可达到93．26 U／mL。
이용상압실온등리자체유변기술대산포도당양화매적흑곡매균주진행유변처리，통과평판사선이급요병복사선출8주매활교고적균주，기중산매활력최고적돌변주위PCTC-8，매활체도14．36 U／mL，교초시균주적매활제고료117．25％。연후재우화배양기적기출지상통과단인소실험대유변균주적발효조건진행우화，최종학정최우적발효조건위：접충량10％，장액량30 mL，충령24 h，발효시간48 h，전속225 r／min，재차조건하최고매활가체도93．26 U／mL。
An atmospheric and room temperature plasma (ARTP)jet was used to mutagenize glucose oxidase-producing strains from Aspergillus niger. Eight strains which had higher glucose oxidase production capacity were selected through plate screening and flask fermentation experiments. The glucose oxidase activity of the best producer,strain PCTC-8, reached 14. 36 U/ mL,which was higher 117. 25% than that of original strain. Effects of the inoculum size,seed age,ro-tation speed,fermentation time and cultural medium volume on the glucose oxidase activity of the mutant strain was inves-tigated carefully by signal factor experiment. The optimum fermentation conditions of PCTC-8 to produce glucose oxidase were as follows:inoculum size 10%,seed age 24 h,rotation speed 225 r/min,fermentation time 48 h and cultural medium volume 30 mL,respectively. Under these conditions the glucose oxidase activity of the best producer was as high as 93. 26 U/mL.