工业微生物
工業微生物
공업미생물
INDUSTRIAL MICROBIOLOGY
2015年
1期
56-61
,共6页
耐铜菌株%筛选%培养条件%Cu2+
耐銅菌株%篩選%培養條件%Cu2+
내동균주%사선%배양조건%Cu2+
copper-resistant strain%screening%cultivation condition%Cu2+
为了筛选和开发能够治理重金属污染的微生物资源，运用梯度驯化筛选法和单因素变量法分别对铜矿厂区土壤中的耐铜微生物进行了筛选及其生产培养条件的研究。结果表明，一株耐铜微生物Cladosporium sp．能耐受Cu2＋的浓度达15000 mg／L。在优化的培养时间96 h，转速150 r／min，温度28℃，pH 6．0下该菌的耐铜性可提高3．62倍，达到54300 mg／L，对环境中铜离子的吸附能力可提高2．27倍，达到38．19 mg／g。该菌对重金属Cu2＋有非常高的耐受和吸附特性，可作为今后微生物治理重金属污染的优良备选菌种，其耐重金属基因也可作为基因工程的实验研究材料。
為瞭篩選和開髮能夠治理重金屬汙染的微生物資源，運用梯度馴化篩選法和單因素變量法分彆對銅礦廠區土壤中的耐銅微生物進行瞭篩選及其生產培養條件的研究。結果錶明，一株耐銅微生物Cladosporium sp．能耐受Cu2＋的濃度達15000 mg／L。在優化的培養時間96 h，轉速150 r／min，溫度28℃，pH 6．0下該菌的耐銅性可提高3．62倍，達到54300 mg／L，對環境中銅離子的吸附能力可提高2．27倍，達到38．19 mg／g。該菌對重金屬Cu2＋有非常高的耐受和吸附特性，可作為今後微生物治理重金屬汙染的優良備選菌種，其耐重金屬基因也可作為基因工程的實驗研究材料。
위료사선화개발능구치리중금속오염적미생물자원，운용제도순화사선법화단인소변량법분별대동광엄구토양중적내동미생물진행료사선급기생산배양조건적연구。결과표명，일주내동미생물Cladosporium sp．능내수Cu2＋적농도체15000 mg／L。재우화적배양시간96 h，전속150 r／min，온도28℃，pH 6．0하해균적내동성가제고3．62배，체도54300 mg／L，대배경중동리자적흡부능력가제고2．27배，체도38．19 mg／g。해균대중금속Cu2＋유비상고적내수화흡부특성，가작위금후미생물치리중금속오염적우량비선균충，기내중금속기인야가작위기인공정적실험연구재료。
In order to exploit microbial resources for handling the metal pollution,the screening of copper-resistant strains and their culture conditions were investigated by using gradient domestication screening method and single factor experi-ment,respectively. The results indicated that the resistant Cu2+ concentration of one Cladosporium sp. strain could reached 15 000 mg/L. Under the optimized conditions including incubation for 96 h at 150 r/min,28℃ and pH 6. 0,the copper resistance of this strain could increased by 3. 62 times,up to 54 300 mg/L. And the environmental copper ion adsorption capacity increased by 2. 27 times,as high as 38. 19 mg/g. Not only for handling the metal pollution,its heavy metal-resistant gene could also be used as genetic engineering material.
